In jurisdictions where limited use of compressed air for cleaning is allowed (British Columbia, New Brunswick, North West Territories, Nova Scotia, Nunavut, Ontario, Yukon, and federal regulations), additional safety control measures are required. The device must be specifically designed to safely clean a person or surface, and personal protective equipment (PPE) must be worn.

In many Canadian jurisdictions, cleaning with compressed air is prohibited (not allowed) by law. Alberta, Newfoundland and Labrador, Prince Edward Island, Quebec, and Saskatchewan specifically mention that compressed air shall not be used to clean clothing worn by a worker, to clean a person, or be directed towards a worker.  In Prince Edward Island it is prohibited to disconnect air lines from air-operated tools for this purpose.

Whatarethe3objectivelenses ona microscope

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These semi-apochromat long-working distance water-dipping objectives for electrophysiology deliver flat images for DIC and fluorescence imaging from the visible range to the near-infrared. Their high NA and low magnification enables bright, precise macro/micro fluorescence imaging for samples such as brain tissue.

Whatisobjective lensinmicroscope

Designed for low-magnification, macro fluorescence observation, this semi-apochromat objective offers a long working distance, a high NA, and high transmission of 340 nm wavelength light.

These extended apochromat objectives offers a high numerical aperture (NA), wide homogenous image flatness, and 400 nm to 1000 nm chromatic aberration compensation. They enable high-resolution, bright image capture for a range of applications, including brightfield, fluorescence, and confocal super resolution microscopy.

These apochromat objectives are dedicated to Fura-2 imaging that features high transmission of 340 nm wavelength light, which works well for calcium imaging with Fura-2 fluorescent dye. They perform well for fluorescence imaging through UV excitation.

For clinical research requiring polarized light microscopy and pathology training, these achromat objectives enable transmitted polarized light observation at an affordable cost.

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The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

Ocularlens microscope

Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

Designed for phase contrast observation of cell cultures in transmitted light, these achromat objectives combine field flatness and easy focusing with cost efficiency. They are well suited for routine microscopy demands.

When using compressed air to clean combustible dusts, potential ignition sources in the dust removal area must be controlled. All sources of ignition must be eliminated, and all machinery and equipment must be de-energized or rated for safe use in atmospheres containing combustible dusts.

Cleaning with compressed air may also be prohibited when there is a risk of the worker being injured, risk of fire or explosion, or use would result in airborne concentrations of hazardous materials that exceed occupational exposure limits or other listed values.

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This super-corrected apochromat objective corrects a broad range of color aberrations to provide images that capture fluorescence in the proper location. Delivering a high degree of correction for lateral and axial chromatic aberration in 2D and 3D images, it offers reliability and accuracy for colocalization analysis.

Objective lensmagnification

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For phase contrast observation of cell cultures, these universal semi-apochromat objectives provide long working distances and flat images with high transmission up to the near-infrared region. They help you achieve clear images of culture specimens regardless of the thickness and material of the vessel.

Designed for clinical research and routine examination work in the laboratory, these achromat objectives provide the level of field flatness required for fluorescence, darkfield, and brightfield observation in transmitted light.

No. Under no circumstances should anyone use compressed air to clean off clothing or any part of the body. Although many people know using compressed air to clean debris or clothes can be hazardous, it is still used because of old habits and the easy availability of compressed air in many workplaces. However, cleaning objects, machinery, bench tops, clothing and other things with compressed air is dangerous. Injuries can be caused by the air jet and by particles made airborne (re-entering the air). Many workplace injuries occur due to the misuse of compressed air.

These semi-apochromat objectives enable phase contrast observation while providing a high level of resolution, contrast, and flatness for unstained specimens.

First, compressed air is extremely forceful. Depending on its pressure, compressed air can dislodge particles. These particles are a danger since they can enter your eyes or abrade the skin. The possible damage would depend on the size, weight, shape, composition, and speed of the particles. The pressure used to remove the particles from machines and surfaces is also strong enough to blow the filings, shavings, chips, and particles of metal into the eyes, ears, or skin of people. Compressed air can enter the body where the skin is not present (i.e., ear, nose, rectum or any scratch or puncture in the skin, however small) and can cause damage. There have also been reports of hearing damage caused by the pressure of compressed air and by its sound.

Typesof objectivelenses

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To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

Many microscopes have several objective lenses that you can rotate the nosepiece to view the specimen at varying magnification powers. Usually, you will find multiple objective lenses on a microscope, consisting of 1.25X to 150X.

Fourth, when combustible dust becomes airborne there is a risk of fire or explosion. Sources of ignition (sparks, energized machinery) can ignite the airborne combustible dust causing injury, death, and property damage.

In addition, air guns should also be used with some local exhaust ventilation or facilities to control the generation of airborne particulates. When compressed air cleaning is unavoidable, hazards can be reduced by making adjustments to the air gun such as:

These super apochromat objectives provide spherical and chromatic aberration compensation and high transmission from the visible to the near infrared. Using silicone oil or water immersion media, which have refractive indexes closely matching that of live cells, they achieve high-resolution imaging deep in living tissue.

These extended apochromat objectives offer high NA, wide homogenous image flatness, 400 nm to 1000 nm chromatic aberration compensation, and the ability to observe phase contrast. Use them to observe transparent and colorless specimens such as live cells, biological tissues, and microorganisms.

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For use without a coverslip or cover glass, these objectives prevent image deterioration even under high magnification, making them well suited for blood smear specimens. They also feature extended flatness and high chromatic aberration correction.

101. (2) Where compressed air is used to clean a surface or person, an employer shall ensure that the device that is used to deliver the air is

Other legislation may apply to specific hazardous materials. For example, cleaning with compressed air is prohibited  when working with asbestos (British Columbia, Manitoba, New Brunswick, North West Territories, Nunavut, Ontario, and federal regulations), respirable crystalline silica and rock dust (British Columbia), and mould contamination (Manitoba). Using compressed air for clean-up of debris containing lead is prohibited in British Columbia, and controlled in the Northwest Territories and Nunavut (additional safety control measures are required). Ontario Fire Code regulations prohibit the use of compressed air to clean combustible dusts from surfaces, unless additional safety control measures are used to prevent fire and explosion.

Second, the compressed air itself is also a serious hazard. On rare occasions, some of the compressed air can enter the blood stream through a break in the skin or a body opening. An air bubble in the blood stream is known medically as an embolism, a dangerous medical condition in which a blood vessel is blocked, in this case, by an air bubble. An embolism of an artery can cause coma, paralysis or death depending upon its size, duration, and location. While air embolisms are usually associated with incorrect diving procedures, they are possible with compressed air due to high pressures. While this seems improbable, even a small quantity of air or other gas in the blood can quickly be fatal.

Enabling tissue culture observation through bottles and dishes, these universal semi-apochromat objectives feature a long working distance and high contrast and resolution. Providing flat images and high transmission up to the NIR region, they are well suited for brightfield, DIC, and fluorescence observation.

Microscopeparts

(a) commercially manufactured and approved in the manufacturer's specifications for the purpose of cleaning a surface or person with compressed air; or

These semi-apochromat and achromat objectives are designed for integrated phase contrast observation of cell cultures. They are used in combination with a pre-centered phase contrast slider (CKX3-SLP), eliminating centering adjustments when changing the objective magnification.

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Objective lensfunction

Some federal regulations limiting the use of compressed air for cleaning are sector-specific, including aviation, maritime operations, oil and gas, offshore marine installations, and on-board trains.

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Use wet sweeping techniques, sweeping compounds, or vacuum cleaners equipped with special filters or other devices to prevent dust from being recirculated into the air.

Microscope objectives come in a range of designs, including apochromat, semi-apochromat, and achromat, among others. Our expansive collection of microscope objectives suits a wide variety of life science applications and observation methods. Explore our selection below to find a microscope objective that meets your needs. You can also use our Objective Finder tool to compare options and locate the ideal microscope objective for your application.

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Personal protection equipment (PPE) must be worn to protect the worker's body, especially the eyes, against particles and dust under pressure. Respiratory protection should also be used if there is a risk that hazardous particulate matter will be inhaled.

Note: Maximum air pressure limits are legislated by British Columbia (70 kPa/10 psig), New Brunswick (69 kPa),North West Territories (68.9 kPa/10 psi), Nunavut (68.9 kPa/10 psi), Yukon (69 kPa/10 psi), and where permitted under federal regulations (69 kPa/10 psi). In Quebec, compressed air used to clean equipment or machines must be less than 200 kPa, unless inside a specially designed enclosure.

Use effective guarding methods that prevent a chip or particle (of any size) from being blown into the eyes or unbroken skin of the operator or other workers nearby. You may also use barriers, baffles, or screens to protect other workers near the operator if there is a risk of exposure.

Designed for clinical research and routine examination in labs using phase contrast illumination, these achromat objectives offer excellent field flatness.

For relief contrast observation of living cells, including oocytes, in plastic vessels using transmitted light, these achromat objectives provide excellent field flatness.

Offering our highest numerical aperture values, these apochromat objectives are optimized for high-contrast TIRF and super resolution imaging. Achieve wide flatness with the UPLAPO-HR objectives’ high NA, enabling  real-time super resolution imaging of live cells and micro-organelles.

Optimized for polarized light microscopy, these semi-apochromat objectives provide flat images with high transmission up to the near-infrared region of the spectrum. They are designed to minimize internal strain to meet the requirements of polarization, Nomarski DIC, brightfield, and fluorescence applications.

What does thestagedoona microscope

Cleaning of surfaces, structures, materials, machinery, work benches, floors, etc. may also be specifically prohibited.

For relief contrast observation of living cells, including oocytes, in plastic vessels, our universal semi-apochromat objectives feature a long working distance. These also provide high image flatness and high transmission up to the near-infrared region.

For high-performance macro-observation, these apochromat objectives provide sharp, clear, flat images without color shift, achieving high transmission up to the near-infrared region of the spectrum. They perform well for fluorescence, brightfield, and Nomarksi DIC observations.

Unfortunately, horseplay has been a cause of some serious workplace accidents caused by individuals not aware of the hazards of compressed air, or proper work procedures.

chip guards or curtains that can deflect flying dust or debris, extension tubes that provide the worker a safer working distance, or air guns equipped with injection exhausts and particle collection bags.

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Third, using air to clean forces the dirt and dust particles into the air, making these contaminants airborne and creating a respiratory hazard.

This semi-apochromat objective series provides flat images and high transmission up to the near-infrared region of the spectrum. Acquiring sharp, clear images without color shift, they offer the desired quality and performance for fluorescence, brightfield, and Nomarksi DIC observations.

Unsure of what microscope objective is right for you? Use our guide on selecting the right microscope objective to weigh your options.

Optimized for multiphoton excitation imaging, these objectives achieve high-resolution 3D imaging through fluorescence detection at a focal point of a large field of view. They enable high-precision imaging of biological specimens to a depth of up to 8 mm for in vivo and transparent samples.