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Depth of field microscopeformula

Depth of field refers to the range within a microscope or any optical system where objects appear in focus. This concept is crucial because it affects how much of the specimen is clearly visible at once, impacting the quality of the observation. A greater depth of field allows for more of the sample to be in focus, while a shallower depth may highlight specific details, making it essential for adjusting magnification and focus during microscopic examinations.

The ability to distinguish two closely spaced objects as separate entities, critical for observing fine details under a microscope.

An opening in the optical system that controls the amount of light that enters, affecting both depth of field and resolution.