For relief contrast observation of living cells, including oocytes, in plastic vessels using transmitted light, these achromat objectives provide excellent field flatness.

Optical metrologysystems

In conclusion, optical metrology is an essential field that underpins numerous industries. It enables us to harness the power of light, correct imperfections, and unlock the full potential of optical systems. The applications of optical metrology are vast and continue to grow as technology advances, making it a pivotal area of scientific research and development.

These extended apochromat objectives offer high NA, wide homogenous image flatness, 400 nm to 1000 nm chromatic aberration compensation, and the ability to observe phase contrast. Use them to observe transparent and colorless specimens such as live cells, biological tissues, and microorganisms.

Offering our highest numerical aperture values, these apochromat objectives are optimized for high-contrast TIRF and super resolution imaging. Achieve wide flatness with the UPLAPO-HR objectives’ high NA, enabling  real-time super resolution imaging of live cells and micro-organelles.

Designed for clinical research and routine examination in labs using phase contrast illumination, these achromat objectives offer excellent field flatness.

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Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

OM is the science and art of measuring and characterizing optical systems, including light sources, lenses, mirrors, and more. It plays a crucial role in ensuring the accuracy and reliability of optical devices, from microscopes to telescopes. This type of metrology encompasses a wide range of techniques and instruments, all designed to quantify and understand the behavior of light.

This super-corrected apochromat objective corrects a broad range of color aberrations to provide images that capture fluorescence in the proper location. Delivering a high degree of correction for lateral and axial chromatic aberration in 2D and 3D images, it offers reliability and accuracy for colocalization analysis.

This semi-apochromat objective series provides flat images and high transmission up to the near-infrared region of the spectrum. Acquiring sharp, clear images without color shift, they offer the desired quality and performance for fluorescence, brightfield, and Nomarksi DIC observations.

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Designed for phase contrast observation of cell cultures in transmitted light, these achromat objectives combine field flatness and easy focusing with cost efficiency. They are well suited for routine microscopy demands.

Optimized for multiphoton excitation imaging, these objectives achieve high-resolution 3D imaging through fluorescence detection at a focal point of a large field of view. They enable high-precision imaging of biological specimens to a depth of up to 8 mm for in vivo and transparent samples.

In the modern world, displays are ubiquitous, from smartphones to large-scale digital signage. Display metrology is a specialized branch of metrology that focuses on the measurement and evaluation of display devices. This includes assessing parameters like color accuracy, luminance, and uniformity, which are critical for creating high-quality displays.

Shack-Hartmann wavefront sensors work on a simple principle. They capture an incoming wavefront, typically from a telescope or camera, and split it into an array of small lenslets. Each lenslet focuses a portion of the incoming light onto a corresponding detector. By analyzing the displacement of these focal spots, the sensor can calculate the wavefront’s shape and determine aberrations.

The metrology of light involves the precise measurement of various optical properties of light, such as intensity, wavelength, polarization, and phase. This is essential for applications in fields like astronomy, telecommunications, and laser technology, where the quality and characteristics of light must be controlled and optimized for specific purposes.

These semi-apochromat objectives enable phase contrast observation while providing a high level of resolution, contrast, and flatness for unstained specimens.

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Designed for low-magnification, macro fluorescence observation, this semi-apochromat objective offers a long working distance, a high NA, and high transmission of 340 nm wavelength light.

Unsure of what microscope objective is right for you? Use our guide on selecting the right microscope objective to weigh your options.

For high-performance macro-observation, these apochromat objectives provide sharp, clear, flat images without color shift, achieving high transmission up to the near-infrared region of the spectrum. They perform well for fluorescence, brightfield, and Nomarksi DIC observations.

For relief contrast observation of living cells, including oocytes, in plastic vessels, our universal semi-apochromat objectives feature a long working distance. These also provide high image flatness and high transmission up to the near-infrared region.

The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

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For phase contrast observation of cell cultures, these universal semi-apochromat objectives provide long working distances and flat images with high transmission up to the near-infrared region. They help you achieve clear images of culture specimens regardless of the thickness and material of the vessel.

These apochromat objectives are dedicated to Fura-2 imaging that features high transmission of 340 nm wavelength light, which works well for calcium imaging with Fura-2 fluorescent dye. They perform well for fluorescence imaging through UV excitation.

These super apochromat objectives provide spherical and chromatic aberration compensation and high transmission from the visible to the near infrared. Using silicone oil or water immersion media, which have refractive indexes closely matching that of live cells, they achieve high-resolution imaging deep in living tissue.

Designed for clinical research and routine examination work in the laboratory, these achromat objectives provide the level of field flatness required for fluorescence, darkfield, and brightfield observation in transmitted light.

Many microscopes have several objective lenses that you can rotate the nosepiece to view the specimen at varying magnification powers. Usually, you will find multiple objective lenses on a microscope, consisting of 1.25X to 150X.

Deep learning inopticalmetrology: a review

Microscope objectives come in a range of designs, including apochromat, semi-apochromat, and achromat, among others. Our expansive collection of microscope objectives suits a wide variety of life science applications and observation methods. Explore our selection below to find a microscope objective that meets your needs. You can also use our Objective Finder tool to compare options and locate the ideal microscope objective for your application.

To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

Shack-Hartmann wavefront sensors are invaluable tools in optical metrology. They find applications in adaptive optics systems, medical imaging, and more. They enable the real-time monitoring and correction of optical aberrations, leading to improved imaging quality. These sensors are used in fields as diverse as ophthalmology, where they enhance vision correction procedures, to astronomy, where they sharpen the images obtained from telescopes.

Optical metrologyservices

Optical metrology, an intriguing field of scientific measurement, delves deep into the intricacies of light and its properties. In this article, we will explore the meaning of optical metrology, the metrology of light, the applications of Shack-Hartmann wavefront sensors, their principles, optical measurement methods, and the significance of display metrology. To further enhance your understanding of this fascinating discipline, we will refer to Imagine Optic, a renowned leader in the field of OM, for insights into their innovative solutions.

These semi-apochromat long-working distance water-dipping objectives for electrophysiology deliver flat images for DIC and fluorescence imaging from the visible range to the near-infrared. Their high NA and low magnification enables bright, precise macro/micro fluorescence imaging for samples such as brain tissue.

For use without a coverslip or cover glass, these objectives prevent image deterioration even under high magnification, making them well suited for blood smear specimens. They also feature extended flatness and high chromatic aberration correction.

These semi-apochromat and achromat objectives are designed for integrated phase contrast observation of cell cultures. They are used in combination with a pre-centered phase contrast slider (CKX3-SLP), eliminating centering adjustments when changing the objective magnification.

Optimized for polarized light microscopy, these semi-apochromat objectives provide flat images with high transmission up to the near-infrared region of the spectrum. They are designed to minimize internal strain to meet the requirements of polarization, Nomarski DIC, brightfield, and fluorescence applications.

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Enabling tissue culture observation through bottles and dishes, these universal semi-apochromat objectives feature a long working distance and high contrast and resolution. Providing flat images and high transmission up to the NIR region, they are well suited for brightfield, DIC, and fluorescence observation.

For a deeper understanding of optical metrology and its practical applications, you can explore Imagine Optic‘s offerings. Their MESO Metrology System provides cutting-edge solutions for characterizing optical surfaces with nanometer precision. The Optical Engineer Companion, another innovative product, offers valuable tools for optical designers.

These extended apochromat objectives offers a high numerical aperture (NA), wide homogenous image flatness, and 400 nm to 1000 nm chromatic aberration compensation. They enable high-resolution, bright image capture for a range of applications, including brightfield, fluorescence, and confocal super resolution microscopy.

Optical measurement methods include a wide array of techniques, such as interferometry, spectrometry, and imaging. These methods are used to measure various optical properties, including reflectance, transmittance, and polarization. Optical metrologists employ these techniques to ensure the quality and functionality of optical components.

For more information on optical metrology and its applications, please visit Imagine Optic’s website and explore their products, further pushing the boundaries of optical measurement and technology.

For clinical research requiring polarized light microscopy and pathology training, these achromat objectives enable transmitted polarized light observation at an affordable cost.