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Raman spectroscopyprinciple

A.D. Cutler, L.M.L. Cantu, E.C.A. Gallo, R. Baurle, P.M. Danehy, R. Rockwell, C. Goyne, J. McDaniel, AIAA J. 53, 2762 (2015)

Mazza, F., Castellanos, L., Kliukin, D., Bohlin, A. (2024). Coherent Anti-Stokes Raman Spectroscopy (CARS). In: Singh, D.K., Kumar Mishra, A., Materny, A. (eds) Raman Spectroscopy. Springer Series in Optical Sciences, vol 248. Springer, Singapore. https://doi.org/10.1007/978-981-97-1703-3_13

what is coherent anti-stokesraman spectroscopy

Vacuum filtration is a critical technique applied in laboratories and industrial processes for the separation of solids from liquids through the use of reduced pressure. Widely utilized in fields such as chemistry, biology, the pharmaceutical industry, and environmental analysis, this method efficiently removes particles, clarifies solutions, and isolates precipitates or solid residues. Also known as negative pressure filtration, its fundamental principle involves creating negative pressure beneath the membrane, leveraging the pressure differential to accelerate liquid flow and achieve effective solid-liquid separation. A typical vacuum filtration setup includes the following components.

Coherent anti-Stokes Raman scattering (CARS) spectroscopy has been used to provide gas-phase quantitative scalar information (e.g. temperature, density, and species concentrations) for more than 5 decades. This technique is renowned for its ability to realize non-intrusive in-situ measurements in harsh environments with excellent spatial and temporal resolution and has become an important tool in multiple energy and combustion science applications, where high-fidelity data are needed. CARS is a non-linear optical process, where the signal originates from the coupling of multiple laser fields to the internal energy states of the probed molecules. This interaction results in excellent chemical specificity, while temperature information is obtained through the direct retrieval of the population distribution on the CARS signal spectrum. CARS thus represents the state-of-the-art in gas-phase thermometry, with unmatched accuracy and precision. The strong “laser-like” signal, which can be detected remotely from where it is generated, makes it also suited for extremely harsh and luminous environments such as flames and plasmas. The present chapter summarizes the fundamentals of gas-phase CARS and discusses a number of most recent advancements: i.e. single-shot CARS imaging, new light sources for ultrabroadband CARS, and simultaneous referencing of the femtosecond (impulsive) excitation efficiency. These recent developments open for interesting possibilities of using CARS in new type of experiments, with coverage of in principle all Raman active modes, obtained with space-time correlated resolution, and improved significance in the delivered data.

Anti stokes raman spectroscopyprinciple

Perfect vacuum or 100% vacuum is a zero reference pressure. In practice, a perfect vacuum is impossible to obtain. The two tables below are for reference only when selecting vacuum pump related products. It is the sole responsibility of users who uses these tables to select their products. Any improper selection or use of the table may cause any damage or injury. Please consult with your own subject matter expert for advice on how to select appropriate vacuum.

Anti stokes raman spectroscopywikipedia

D. Kliukin, A. Bohlin, Patent P126758NL00/Simultaneous Space-Time Resolution Broadband Coherent Raman Microscope with In-Situ Spectral Referencing (2021)

F. Mazza, O. Thornquist, L. Castellanos, T. Butterworth, C. Richard, V. Boudon, A. Bohlin, J. Chem. Phys. 158, 094201 (2023)

coherent anti-stokesraman spectroscopyof single and multi-layer graphene

Tags Sample Preparation, Drug Delivery, Cell Culture, Vacuum Pump

M. Scherman, R. Santagata, E. Lin, P. Nicolas, J.-P. Faleni, A. Vincent-Randonnier, P. Cherubini, F. Guichard, A. Mohamed, D. Gaffie, B. Attal-tretout, A. Bresson, J. Raman Spectrosc. 52, 1643 (2021)

G. Herzberg, Molecular Spectra and Molecular Structure. I. Spectra of Diatomic Molecules, 2nd edn. (Van Nostrand Reinhold Company, Inc., 1950)

Anti stokes raman spectroscopynotes

Two charts can be found below in Figure 1 and Figure 2. At 40% Vacuum, the absolute vacuum pressure reads 17.95 in Hg while the absolute vacuum pressure is about 5.98 in Hg at 80% vacuum. Similarly in Figure 2, the gauge vacuum pressure is relative to atmosphere pressure (29.92 in Hg). Thus, at 40% vacuum, we can directly subtract the atmosphere pressure (22.9 in Hg) from the absolute pressure ( 17.95 - 29.92 = -11.97 in Hg gauge). At 80% vacuum, the relative vacuum can be calculated in the same way (5.98 - 29.92 = - 23.94 in Hg gauge).

When using a programmable spin coater, it's important to follow certain precautions to ensure safety, optimal performance, and longevity of the equipment. Additionally, regular preventative maintenance is crucial to address wear and tear, prevent malfunctions, and prolong the life of the spin coater. Here are some general precautions and preventative maintenance guidelines:

Z. Liu, J. Yao, H. Zhang, B. Xu, J. Chen, F. Zhang, Z. Zhang, Y. Wan, W. Chu, Z. Wang, Y. Cheng, Phys. Rev. A 101, 043404 (2020)

The Laboratory Vacuum Filtration System emerges as a versatile and indispensable tool, excelling in precise separation and filtration processes. Operating on the principle of creating a pressure differential across the filter medium by evacuating the air beneath it, this innovative system significantly enhances filtration rates. Intricately designed, the vacuum filtration system optimizes procedures for isolating solids from liquid samples, separating precipitates from solutions, and preparing samples for comprehensive analyses.

Anti stokes raman spectroscopyformula

A vacuum pump is a mechanical device that is used to create a vacuum by removing gas molecules from a sealed volume in order to create vacuum. There are several types of vacuum pumps available, each with its own set of features and capabilities. Some common types of vacuum pumps include: rotary vane pumps, diaphragm pumps, piston pumps, scroll umps and diffusion pumps.

The vacuum pump in a spin coater is typically used to remove air from the space between the substrate and the chuck, ensuring good contact and adhesion between the substrate and the coating material. The vacuum helps to eliminate air pockets and enhance the uniformity of the coating. Here's a general guide on how to use the vacuum pump in a spin coater and the practical tips and considerations.

coherent anti-stokesramanscattering microscopy

The Vacuum Manifolds have been specifically designed to enhance the efficiency of high-throughput processes such as nucleic acid purification, solid phase extraction, protein precipitation, Oligo synthesis, and various other applications. These manifolds are tailored to accommodate 48/96/384 well plates and Luer-inlet columns, streamlining procedures by eliminating the need for repetitive pipetting and centrifugation inherent in traditional nucleic acid extraction methods.

A programmable spin coater is a laboratory instrument used in the deposition of thin films onto substrates through a process called spin coating. Spin coating is a widely used technique in the fields of material science and microfabrication to create thin and uniform films of various materials, such as polymers, metals, and ceramics, on a substrate.

S. Legros, C. Brunet, P. Domingo-Alvarez, P. Malbois, E. Salaun, G. Godard, M. Caceres, B. Barviau, G. Cabot, B. Renou, G. Lartigue, V. Moureau, S. Puggelli, S. Richard, M. Abdelkrim, F. Grisch, Combust. Flame 224, 273 (2021)