NOTE: If a 1/3” inch camera (6mm diagonal) is used on the 0.5X microscope adaptor you can apply the ratio of 6/8 for the reduction in the captured field.

Objectivelensmicroscopefunction

TIP: On the trinocular version of the PZMIII or PZMIV stereo microscope with the standard configuration (1.0X objective, 10X eyepieces) and with the optimal camera adaptor (0.5X on a ½” CCD camera) the video capture field of view is up to 40% less than the visual field. By using a 0.5X objective with 20X eyepieces the video capture area doubles, and the resulting video capture more closely matches the visual field of view.

Problem: The PZMIII or PZMIV stereo zoom microscope normally comes with a 1.0X objective and a 10X pair of eyepieces. The magnification is 6X to 50X, however the concept of magnification is difficult to visualize. Let's discuss what can be seen at the two zoom extremes. Imagine the visual circle to be a range of 34–4.2 mm. This microscope has a working distance of 100mm. Researchers working with small animals will have difficulty working in this tight space.

MicroscopeObjectives magnification

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Added to the feature package is an automatic electronic zoom function from 15 to 50 degrees.The elektraLite VW Fresnel’s rugged design and 600 watt output make it ideal for use in a wide variety of public spaces including classrooms, galleries, worship spaces, event spaces, live entertainment, broadcast and film studios.

Solution: Instead of the standard configuration, setup the microscope with a 0.5X objective to increase the working distance to 187 mm. The result of using this lower power objective is that the magnification range decreases by one half and at the same time the field of view double. To restore the microscope system to the original condition (magnification and field of view), replace the 10X eyepieces with 20X eyepieces. The use of these two options restores the visual field of view and magnification range back to the original condition with the added benefit of a larger working distance.

What is the objective in a microscopeexplain

A variety of microscope objectives are available. All objectives use lenses to focus light. Light is broken down into various wavelengths (colors) as it travels through a lens. The various wavelengths have different focal points. That means that red, green and blue appears to focus at different points. This is called chromatic aberration. Spherical aberrations are focal mismatches caused by the shape of the lens. Quality lenses are designed correct for chromatic and spherical aberration to bring the primary colors to a common focal point. These terms may help you determine the best objective for your application:

Whatdoesthestage do ona microscope

Fluorite or semi-apochromat objectives–These lenses are chromatically corrected for red and blue, and the green focus is also close. They are spherically corrected for blue and green. This objective is better suited for color viewing or recording than achromatic objectives.

Using the latest led technology the elektraLite VW 600w Fresnel incorporates a powerful 600watt COB led with a CRI of 95.The COB single source led is far superior to any multi source led, not only by its power and efficiency, but also the perfect cuts when using barn doors.

The elektraLite VW 600w Fresnel brings a new look to the Fresnel world. Gone are the projector or discharge lamp problems.No more hot surfaces. The fixture has elektraLite’s exclusive “cool to touch” housing.No more lamp changes because the color temperature changed every few hours as the lamp aged.

Parts ofa microscope

Plan objective–These objectives produces a flat image across the field of view. The three objectives discussed above all produce a curved image. A plan-achromat, plan-fluorite or plan-apochromat are corrected.

Apochromatic objective–This is the most expensive objective. It is chromatically adjusted for four colors (deep blue, blue, green and red) and spherically corrected for deep blue, blue and sometimes green. This is the best choice for color viewing. These have a higher numerical aperture (N.A.) than achromats or fluorites.

The magnification of the image depends on the combination of the eyepiece and the objective used. This combination also affects the field of view. This example shows how these factors inter-relate.

Infinity Correction–When measuring from the back end of the objective to the primary focal plane, many microscopes are limited to a specific distance (160mm). More expensive microscope use a different series of lenses, prisms and mirrors to allow for an "infinite" distance between those two points. This is called infinity correction.

The first image shows the eyepiece view when using a 1.0X objective with a 10X eyepiece. It has a 34mm field of view. The second image shows the video field of view of about 16–4.7mm (COLCAM-NTSC camera with a 0.5X coupler). The third image shows the video view that approximates the eyepiece view. It uses a 0.5X objective with a 20X eyepiece.

Types ofmicroscopeobjectives

The choice of 600w for power allows the Variable White to blend perfectly with our 300w WW or CW fresnels and our 300w WW or CW ellipsoidal fixtures.

The Fresnel VW can output a full 300w of warm white and cool white. The color temperature always evenly displayed across the whole field with no hot spots. The dmx profile of the fixture allows the lighting designer or programmer to instantly dial up color temperatures from 3000k to 6000k in 100k increments.

A simple to use onboard control interface allows for easy control for console-free static operation. The elektraLite VW Fresnel has 5pin XLR connectors for both in and thru control using DMX/RDM, Barn doors, a single yoke, safety eye bolt and safety cable are included. Industry standard Powercon in and out connectors allow quick connection and daisy chaining of fixtures.

Achromatic objectives–This objective brings red and blue light to a common focus, and is corrected for spherical aberrations for green. It is excellent for black and white viewing. If an objective is not labeled, it is achromatic.