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Raman Spectroscopy offers numerous advantages. Since Raman instruments use lasers in the visible region, flexible silica fiber optic cables can be used to excite the sample and collect the dispersed radiation, and these cables can be quite long if necessary. Since visible light is used, glass or quartz can be used to hold samples. In the study of chemical reactions, this means that the Raman probe can be inserted into a reaction or can collect Raman spectra though a window, for example in an external reaction sample loop or flow cell. The latter approach eliminates the possibility of sample stream contamination. The ability to use quartz or Hi-grade Sapphire as a window material means that high pressure cells can be used to acquire Raman spectra of catalytic reactions. In the study of catalysts, operando spectroscopy using the Raman effect is quite useful for studying in situ, real-time reactions on catalytic surfaces. Another advantage of Raman is that hydroxyl bonds are not particularly Raman active, making Raman spectroscopy in aqueous media straightforward. Raman spectroscopy is considered non-destructive, though some samples may be effected by the laser radiation. One consideration that needs to be made when choosing this technique is how fluorescent a particular sample may be. Raman scattering is a weak phenomena and fluorescence can swamp the signal making it difficult to collect quality data. This issue often can be alleviated by using a longer wavelength excitation source.

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Dow researchers have developed an alternate means of producing silicone, based on a precisely controlled polymerization, to yield product with targeted, uniform chain lengths. In this synthesis, a lithium-based reactant serves to open a cyclic tri-siloxane ring, followed by addition of another cyclic siloxane reagent, to yield a monodispersed silicone polymer.

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A modern, compact Raman spectrometer consists of several basic components, including a laser that serves as the excitation source to induce the Raman scattering. Typically, solid state lasers are used in modern Raman instruments with popular wavelengths of 532 nm, 785 nm, 830 nm and 1064 nm. The shorter wavelength lasers have higher Raman scattering cross-sections so the resulting signal is greater, however the incidence of fluorescence also increases at shorter wavelength. For this reason, many Raman systems feature the 785 nm laser. The laser energy is transmitted to and collected from the sample by fiber optics cables. A notch or edge filter is used to eliminate Rayleigh and anti-Stokes scattering and the remaining Stokes scattered light is passed on to a dispersion element, typically a holographic grating. A CCD detector captures the light, resulting in the Raman spectrum. Since Raman scattering yields a weak signal, it is most important that high-quality, optically well-matched components are used in the Raman spectrometer.

When light interacts with molecules in a gas, liquid, or solid, the vast majority of the photons are dispersed at the same energy as the incident photons. This is described as elastic or Rayleigh scattering. A small number of these photons, approximately 1 photon in 10 million will disperse at a different frequency than the incident photon. This process is called inelastic scattering, or the Raman effect, named after Sir C.V. Raman who discovered this and was awarded the 1930 Nobel Prize in Physics for his work. Since that time, Raman has been utilized for a vast array of applications from medical diagnostics to material science and reaction analysis. Raman allows the user to collect the vibrational signature of a molecule, giving insight into how it is put together, as well as how it interacts with other molecules around it.

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Raman spectroscopy is a molecular spectroscopic technique that utilizes the interaction of light with matter to gain insight into a material's make up or characteristics, like FTIR. The information provided by Raman spectroscopy results from a light dispersion process, whereas IR spectroscopy relies on absorption of light. Raman spectroscopy yields information about intra- and inter-molecular vibrations and can provide additional understanding about a reaction. Both Raman and FTIR spectroscopy provide a spectrum characteristic of the specific vibrations of a molecule ("molecular fingerprint') and are valuable for identifying a substance. However, Raman spectroscopy can give additional information about lower frequency modes, and vibrations that give insight into crystal lattice and molecular backbone structure.  Inline Raman spectroscopy is used to monitor crystallization processes and reveal reaction mechanisms and kinetics. Combined with analysis tools, this data enables informed reaction understanding and optimization.

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Traditionally, this analysis has been performed by spectroscopists with expert knowledge in finding key areas of interest and trending these wavenumbers over time. However, advances in software (like the 'Find Trends' feature in iC Raman 7) have enabled this expertise to be automated in a way that experts and non-experts alike can easily extract key information quickly for fast, confident decision making.

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Because Raman is an inherently weak effect, the optical components of a Raman Spectrometer must be well matched and optimized. Also, since organic molecules may have a greater tendency to fluoresce when shorter wavelength radiation is used, longer wavelength monochromatic excitation sources, such as solid state laser diodes that produces light at 785 nm, are typically used.

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Unlike FTIR Spectroscopy that looks at changes in dipole moments, Raman looks at changes in a molecular bonds polarizability. Interaction of light with a molecule can induce a deformation of its electron cloud. This deformation is known as a change in polarizability. Molecular bonds have specific energy transitions in which a change of polarizability occurs, giving rise to Raman active modes.  As an example, molecules that contain bonds between homonuclear atoms such as carbon-carbon, sulfur-sulfur, and nitrogen-nitrogen bonds undergo a change in polarizability when photons interact with them. These are examples of bonds that give rise to Raman active spectral bands, but would not be seen or difficult to see in FTIR.

Designed specifically for chemical and process development, these tools are combined across the powerful iC software platform to provide comprehensive process understanding.

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For a wide range of industries, silicone's diverse properties enable companies to design products with specific, fit-for-purpose characteristics. These products exploit the varied properties of silicone rubbers such as strength, thermal resistivity and stability. Typically, silicone is produced via hydrolysis of a chlorosilane followed with a terminal functional group addition, or through polycondensation of a cyclic siloxane. Each of these methods are equilibrium reactions that produce low-molecular-weight products with a wide range molecular weight distribution.

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From data collection to analysis, ReactRaman™ with iC Raman™ brings compositional analysis to every lab. Automated parameter selection provides accurate data collection, enabling scientists to get confident results. Right first time, every time, in every process with every user.​

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This novel silicone polymerization, which results in monodispersed product with precisely controlled chain lengths, is tracked by ReactRaman, eliminating the delays and reaction uncertainties associated with offline GC analysis. Reaction initiation, progress and kinetics are all readily measured by the Raman method, providing continuous, real time verification that the reaction is proceeding as expected.

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Information-Rich Experimentation​ Data acquisition and analysis is quick and easy with the industry standard iC Software for reaction analysis. iC Software seamlessly incorporates multiple orthogonal data streams to link process variables that drive a comprehensive process understanding.​

When spectrum is collected consistently over the course of an experiment, it can reveal a 'molecular video' that provides key information regarding the kinetics, mechanisms, and form changes during a reaction.

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With respect to reaction analysis, Raman spectroscopy is sensitive to many functional groups but is exceptional when obtaining molecular backbone information, providing its own unique molecular fingerprint. Because Raman utilizes a bonds polarizability and has the potential to measure lower frequency, it is sensitive to crystal lattice vibrations giving the user polymorphic information that can be challenging to obtain by FTIR. This allows Raman to be used very effectively to study crystallization and other complex processes.

Shared Expertise​ Thousands of PAT installations around the world and four decades of experience are built into ReactRaman 802L. Our global expert support team is committed to ensuring users’ success through trainings and application development, in-person or virtual, whenever needed.

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Although Raman and FTIR Spectroscopy give complimentary information and are often interchangeable, there are some practical differences that influence which one will be optimal for a given experiment. Most molecular symmetry will allow for both Raman and IR activity. One special case is if the molecule contains a center of inversion. In a molecule that contains a center of inversion, Raman bands and IR bands are mutually exclusive, i.e. the bond will either be Raman active or it will be IR active but it will not be both. One general rule is that functional groups that have large changes in dipoles are strong in the IR, whereas functional groups that have weak dipole changes or have a high degree of symmetry will be better seen in Raman spectra.