In order to operate the tutorial, use the Laser Excitation slider to toggle through the available laser line wavelength range of 322 (ultraviolet) to 647 (red) nanometers. As the slider is translated, the color of the laser excitation and secondary fluorescence emission beams changes, as does the color of the corresponding bandpass filters and dichromatic mirror. The Scan Rate slider can be utilized to increase or decrease the scan rate, both on the virtual microscope optical train and across the Specimen Image in the right-hand side of the window. The entire microscope assembly can be rotated around the z-axis (through 360-degrees) for a different view by clicking and dragging with the mouse cursor.

Galvanometerscanner

MWIR (Midwave Infrared) Thermal Imaging  Camera Lenses are lenses oriented for operation in the 3-5 micro wavelength infrared spectrum. Compared with LWIR lenses, MWIR detects radiations emitted from heated objects and because MWIR thermal cameras often utilize cooled photon sensors, (often FPA detectors made of HgCdTe or InSb), which exhibit higher sensitivities than the uncooled thermal imagers often utilized in LWIR thermal cameras, MWIR thermal imaging, as a subsequence, has better capabilities to reject thermal noise, and clutter and to discriminate similar temperatures, elevating the target contrast in the image. MWIR lenses are also less susceptible to atmospheric absorption than LWIR lenses, making MWIR lenses thermal lenses recommendable for long-range detection. Shalom EO’s MWIR lenses feature optimized Modulation Transfer Function (MTF) and exceptional Detection, Recognition, and Identification (DRI) functionalities. The lenses, with a broad portfolio of varied specifications, support detection under poor atmospheric/illumination conditions (e.g. in fog/ complete darkness), and even allow invisible objects to be captured (e.g. gas leakage). Shalom EO’s MWIR lenses are an excellent option for thermal imaging in various domains, including industrial processing (high-temperature process monitoring), academic, surveillance, and homeland security (night vision and target detection).

Regarding the MWIR Continuous Zoom Lens Assemblies, all MWIR zoom lens modules boast a maximum magnification of x20, flexible zooming, diffraction-limited MTF, with maintained focus at full zoom range. The lens assemblies are compatible with sensitive cooled detectors, supporting high resolutions and observation at a broad range of distances for various high-end applications. Shalom EO presents our latest released modules: 313-004 EFL90-1100mm f5.5 MWIR Continuous Zoom Long Range Thermal Lenses, and 313-010 EFL75-1100mm f4.0 MWIR Continuous Zoom Long Range Thermal Lenses, both the modules feature an extraordinary focal length upper limit of >1000mm, allowing for a detection range of over 35km to provide users with awareness covering remote distances. The 313-004 module also highlights a lightweight characteristic of 4.2kg, enabling weight-sensitive applications like drones.

Galvanometer

Galvo Scanner

The tutorial initializes with the major components of a scanning confocal laser microscope optical train positioned in the left-hand side of the window. A virtual argon-ion laser beam (default excitation wavelength of 457 nanometers) passes through an excitation filter and dichromatic mirror before reflecting from the surface of scanning mirror number two, which controls the y scan direction. After leaving mirror number two, the beam is reflected by scanning mirror number one (controlling the x scan direction) through the objective and onto the specimen. Secondary fluorescence emitted by the specimen is arbitrarily shifted to higher wavelengths (50 nanometers) in the tutorial, and passes back through the scan mirrors in a process known as descanning before being transmitted through the dichromatic mirror and the barrier filter on to the photomultiplier.

PI galvo

Hangzhou Shalom EO offers off-the-shelf and custom Lenses for Thermal Imaging Cameras. For MWIR Lens Modules, a wide assortment of lenses is available, including MWIR Single FOV Lenses with a designated focal length and FOV each, MWIR Dual FOV Lenses with two selectable focal lengths, and MWIR Continuous Zoom Lens Assemblies which enable continuous shifting of focal lengths, permitting flexible observation over a wide range of distances and real-time detection. Materials with high MWIR transmission including Germanium, ZnSe, Chalcogenide glass, Zinc Sulfide, GaAs, and Silicon are utilized as substrate materials, and anti-flection (AR) coatings, Diamond-Like Carbon (DLC) coating could be deposited according to your requirements.

Over the years, Shalom EO has built a solid reputation as a leading figure manufacturer and supplier of infrared thermal imaging lenses.  Shalom EO's engineers and specialists have succeeded in devising thermal imaging lenses with surpassing optical excellence and durable constructions based on their rich technical knowledge, and our engineers will continue to tailor the optimized design for our customers.

Fast Steeringmirror

Galvomirror

Commercial laser scanning confocal microscopes feature a variety of scanning mirror configurations that often include a relay optical system to assist in generation of the raster pattern. In most cases, the galvanometer-driven scan mirrors are positioned to rotate in mutually perpendicular axes to create a set of telecentric planes. One of the mirrors controls scanning across the x axis, while the other translates the beam along the y axis. The motion of each mirror is coordinated to form the raster pattern, and the scanning speed is regulated by the speed and angular extend of mirror deflection.

MWIR (Midwave Infrared) Thermal Imaging  Camera Lenses are lenses oriented for operation in the 3-5 micro wavelength infrared spectrum. Compared with LWIR lenses, MWIR detects radiations emitted from heated objects and because MWIR thermal cameras often utilize cooled photon sensors, (often FPA detectors made of HgCdTe or InSb), which exhibit higher sensitivities than the uncooled thermal imagers often utilized in LWIR thermal cameras, MWIR thermal imaging, as a subsequence, has better capabilities to reject thermal noise, and clutter and to discriminate similar temperatures, elevating the target contrast in the image. MWIR lenses are also less susceptible to atmospheric absorption than LWIR lenses, making MWIR lenses thermal lenses recommendable for long-range detection. Shalom EO’s MWIR lenses feature optimized Modulation Transfer Function (MTF) and exceptional Detection, Recognition, and Identification (DRI) functionalities. The lenses, with a broad portfolio of varied specifications, support detection under poor atmospheric/illumination conditions (e.g. in fog/ complete darkness), and even allow invisible objects to be captured (e.g. gas leakage). Shalom EO’s MWIR lenses are an excellent option for thermal imaging in various domains, including industrial processing (high-temperature process monitoring), academic, surveillance, and homeland security (night vision and target detection).Hangzhou Shalom EO offers off-the-shelf and custom Lenses for Thermal Imaging Cameras. For MWIR Lens Modules, a wide assortment of lenses is available, including MWIR Single FOV Lenses with a designated focal length and FOV each, MWIR Dual FOV Lenses with two selectable focal lengths, and MWIR Continuous Zoom Lens Assemblies which enable continuous shifting of focal lengths, permitting flexible observation over a wide range of distances and real-time detection. Materials with high MWIR transmission including Germanium, ZnSe, Chalcogenide glass, Zinc Sulfide, GaAs, and Silicon are utilized as substrate materials, and anti-flection (AR) coatings, Diamond-Like Carbon (DLC) coating could be deposited according to your requirements. Over the years, Shalom EO has built a solid reputation as a leading figure manufacturer and supplier of infrared thermal imaging lenses.  Shalom EO's engineers and specialists have succeeded in devising thermal imaging lenses with surpassing optical excellence and durable constructions based on their rich technical knowledge, and our engineers will continue to tailor the optimized design for our customers. Regarding the MWIR Continuous Zoom Lens Assemblies, all MWIR zoom lens modules boast a maximum magnification of x20, flexible zooming, diffraction-limited MTF, with maintained focus at full zoom range. The lens assemblies are compatible with sensitive cooled detectors, supporting high resolutions and observation at a broad range of distances for various high-end applications. Shalom EO presents our latest released modules: 313-004 EFL90-1100mm f5.5 MWIR Continuous Zoom Long Range Thermal Lenses, and 313-010 EFL75-1100mm f4.0 MWIR Continuous Zoom Long Range Thermal Lenses, both the modules feature an extraordinary focal length upper limit of >1000mm, allowing for a detection range of over 35km to provide users with awareness covering remote distances. The 313-004 module also highlights a lightweight characteristic of 4.2kg, enabling weight-sensitive applications like drones.Our lens production line incorporates cutting-edge processes such as CNC fabrication and single-point diamond turning (SPDT) polishing, under stringent QC procedures. The sealings of lens groups are IP67-rated waterproof and dust-tight.  Built-in manual and motorized focus mechanisms are integrated into focal-length-variable lens modules, and compact mechanical designs for lightweight concerns can be designed upon request. In addition, athermalized lens modules to compensate for temperature fluctuations are also accessible both in our stock list and as custom products.

Secondary fluorescence emission gathered from the excited specimen by the objective travels back through the optical system and scanning assembly along the same pathway (coaxial) as the excitation illumination in the descanning process. The emission beam position at the pinhole aperture does not follow the raster scanning pattern (as does the excitation illumination), but remains steady and fluctuates only in intensity as the scanning beam excites fluorophores at varying concentrations in the specimen.

In order to generate a digital image from an extended specimen in laser scanning confocal microscopy, the focused beam is scanned laterally (in the x-y plane) across the specimen surface in a rectangular raster pattern. Modern instruments utilize a scanning mechanism based on two high-speed vibrating mirrors driven by galvanometer motors to produce the scanning pattern. This interactive tutorial explores how the scanning mirrors are coordinated to direct the laser beam into the objective, and then to reflect secondary fluorescence gathered from the specimen back through the optical train to the emission filter.

Our lens production line incorporates cutting-edge processes such as CNC fabrication and single-point diamond turning (SPDT) polishing, under stringent QC procedures. The sealings of lens groups are IP67-rated waterproof and dust-tight.  Built-in manual and motorized focus mechanisms are integrated into focal-length-variable lens modules, and compact mechanical designs for lightweight concerns can be designed upon request. In addition, athermalized lens modules to compensate for temperature fluctuations are also accessible both in our stock list and as custom products.