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13.  0.5mm / 1.5 cells = 0.25mm/neuron or 250 micrometers/ neuron (I estimated that 1.5 neurons would fit across the field of view)

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12. Calculate the field diameter (in millimeters and micrometers) for the 100X objective.  You know that the 10x objective has a field diameter of 2mm

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8. Calculate the field diameter (in millimeters and micrometers) for the 10X objective.  You know that the 4x objective has a field diameter of 6mm or 6000 micrometers.

11. Calculate the field diameter (in millimeters and micrometers) for the 10X objective.  You know that the 40x objective has a field diameter of 0.2mm.

The following problems will help you practice calculating total magnification, field diameter, and estimating cell size.  Answers are on the last page.

15.   3mm / 60 cells = 0.05mm/cell or 50 micrometers/cell (I estimated that 60 cuboidal cells would fit across the field of view)

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14.  0.3mm / 3 cells = 0.1mm/cell or 100 micrometers/cell (I estimated that 3 white blood cells would fit across the field of view)

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10. Calculate the field diameter (in millimeters and micrometers) for the 4X objective.  You know that the 40x objective has a field diameter of 0.3mm.

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7. Calculate the field diameter (in millimeters and micrometers) for the 40X objective.  You know that the 4x objective has a field diameter of 6mm or 6000 micrometers.

16.  5mm / 10 cells = 0.5mm/cell or 500micrometers/cell   (I estimated that 10 squamous cells would fit across the field of view)

This page titled 3.1: Practice for Microscopy Calculations is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by .

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9. Calculate the field diameter (in millimeters and micrometers) for the 125X objective.  You know that the 100x objective has a field diameter of 0.2mm.

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