These extended apochromat objectives offers a high numerical aperture (NA), wide homogenous image flatness, and 400 nm to 1000 nm chromatic aberration compensation. They enable high-resolution, bright image capture for a range of applications, including brightfield, fluorescence, and confocal super resolution microscopy.

These extended apochromat objectives offer high NA, wide homogenous image flatness, 400 nm to 1000 nm chromatic aberration compensation, and the ability to observe phase contrast. Use them to observe transparent and colorless specimens such as live cells, biological tissues, and microorganisms.

Optimized for multiphoton excitation imaging, these objectives achieve high-resolution 3D imaging through fluorescence detection at a focal point of a large field of view. They enable high-precision imaging of biological specimens to a depth of up to 8 mm for in vivo and transparent samples.

Whatisobjectivelens inmicroscope

Exceeding the limit of useful magnification causes the image to suffer from the phenomenon of empty magnification (illustrated in Figure 1(b)), where increasing magnification through the eyepiece or intermediate tube lens only causes the image to become more magnified with no corresponding increase in detail resolution. In contrast, the image shown in Figure 1(a) was captured using the correct objective and eyepiece combination to effectively utilize the numerical aperture to achieve optimum resolution.

Scanningobjectivelens

These apochromat objectives are dedicated to Fura-2 imaging that features high transmission of 340 nm wavelength light, which works well for calcium imaging with Fura-2 fluorescent dye. They perform well for fluorescence imaging through UV excitation.

For relief contrast observation of living cells, including oocytes, in plastic vessels, our universal semi-apochromat objectives feature a long working distance. These also provide high image flatness and high transmission up to the near-infrared region.

The range of useful magnification for an objective/eyepiece combination is defined by the numerical aperture of the microscope optical system. There is a minimum magnification necessary for the detail present in an image to be resolved, and this value is usually rather arbitrarily set as 500 times the numerical aperture (500 x NA) and defined by the equation:

The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

Many microscopes have several objective lenses that you can rotate the nosepiece to view the specimen at varying magnification powers. Usually, you will find multiple objective lenses on a microscope, consisting of 1.25X to 150X.

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Designed for clinical research and routine examination work in the laboratory, these achromat objectives provide the level of field flatness required for fluorescence, darkfield, and brightfield observation in transmitted light.

Whatarethe3objective lenses on a microscope

Optimized for polarized light microscopy, these semi-apochromat objectives provide flat images with high transmission up to the near-infrared region of the spectrum. They are designed to minimize internal strain to meet the requirements of polarization, Nomarski DIC, brightfield, and fluorescence applications.

At high magnifications, the limit of useful magnification is sometimes exceeded in order to view the image more comfortably. This is often the case when small particles or organisms are observed and counted at very high numerical apertures and magnifications. Sharpness in the specimen details is then sacrificed, which usually does not interfere with quantitative analysis of the image.

Microscopeparts

For visual observation, the image of the specimen fine structure must be viewed at an angle slightly larger than the resolving power of the human eye. With a microscope having good illumination, the distance between two resolved points in the specimen viewed at the reference visual distance of 250 millimeters is about 0.15 millimeters, corresponding to a visual acuity angle of about 2 minutes of arc. This limiting angle is restricted by the separation distance of visual elements in the retina, which are spaced about five microns apart.

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At the other end of the spectrum, the maximum useful magnification of an image is usually set at 1000 times the numerical aperture (1000 x NA) as given by the equation above. Magnifications higher than this value will yield no further useful information or finer resolution of image detail, and will usually lead to image degradation. Table 1 catalogs the common objective/eyepiece combinations that lie in the range of useful magnification.

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Types ofobjective lenses

Microscope objectives come in a range of designs, including apochromat, semi-apochromat, and achromat, among others. Our expansive collection of microscope objectives suits a wide variety of life science applications and observation methods. Explore our selection below to find a microscope objective that meets your needs. You can also use our Objective Finder tool to compare options and locate the ideal microscope objective for your application.

These semi-apochromat and achromat objectives are designed for integrated phase contrast observation of cell cultures. They are used in combination with a pre-centered phase contrast slider (CKX3-SLP), eliminating centering adjustments when changing the objective magnification.

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Designed for phase contrast observation of cell cultures in transmitted light, these achromat objectives combine field flatness and easy focusing with cost efficiency. They are well suited for routine microscopy demands.

In order to observe fine specimen detail in the optical microscope, the minute features present must be of sufficient contrast and project an intermediate image at an angle that is somewhat larger than the angular resolving power of the human eye. At a selected numerical aperture, when the microscope provides a magnified image that has a magnitude equal to the resolution limit of the human eye, additional magnification beyond this point does not result in the resolution of even smaller specimen detail.

For high-performance macro-observation, these apochromat objectives provide sharp, clear, flat images without color shift, achieving high transmission up to the near-infrared region of the spectrum. They perform well for fluorescence, brightfield, and Nomarksi DIC observations.

Designed for clinical research and routine examination in labs using phase contrast illumination, these achromat objectives offer excellent field flatness.

To relate the limit of resolution of the eye and the resolving power of the objective, two closely spaced points in the specimen can be considered. If the two points reside at the limit of the objective's resolving power, then:

For use without a coverslip or cover glass, these objectives prevent image deterioration even under high magnification, making them well suited for blood smear specimens. They also feature extended flatness and high chromatic aberration correction.

Offering our highest numerical aperture values, these apochromat objectives are optimized for high-contrast TIRF and super resolution imaging. Achieve wide flatness with the UPLAPO-HR objectives’ high NA, enabling  real-time super resolution imaging of live cells and micro-organelles.

This super-corrected apochromat objective corrects a broad range of color aberrations to provide images that capture fluorescence in the proper location. Delivering a high degree of correction for lateral and axial chromatic aberration in 2D and 3D images, it offers reliability and accuracy for colocalization analysis.

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This semi-apochromat objective series provides flat images and high transmission up to the near-infrared region of the spectrum. Acquiring sharp, clear images without color shift, they offer the desired quality and performance for fluorescence, brightfield, and Nomarksi DIC observations.

Care should be taken in choosing eyepiece/objective combinations to ensure the optimal magnification of specimen detail without adding unnecessary artifacts. For instance, to achieve a magnification of 250x, the microscopist could choose a 25x eyepiece coupled to a 10x objective. An alternative choice for the same magnification would be a 10x eyepiece with a 25x objective. Because the 25x objective has a higher numerical aperture (approximately 0.65) than does the 10x objective (approximately 0.25), and considering that numerical aperture values define an objective's resolution, it is clear that the latter choice would be the best. If photomicrographs of the same viewfield were made with each objective/eyepiece combination described above, it would be obvious that the 10x eyepiece/25x objective duo would produce photomicrographs that excelled in specimen detail and clarity when compared to the alternative combination.

Whatdoesthestagedo on a microscope

World-class Nikon objectives, including renowned CFI60 infinity optics, deliver brilliant images of breathtaking sharpness and clarity, from ultra-low to the highest magnifications.

For phase contrast observation of cell cultures, these universal semi-apochromat objectives provide long working distances and flat images with high transmission up to the near-infrared region. They help you achieve clear images of culture specimens regardless of the thickness and material of the vessel.

High powerobjective microscopefunction

The result is the minimum magnification for visual observation of the finely spaced specimen detail, which is about 500 times the objective numerical aperture. This discussion applies to specimens having medium contrast, but with specimens of higher contrast the two points can be resolved by higher magnifications even if they are closer to each other. In practice, magnifications deviating considerably from the useful magnification range are often employed. For example, very low magnifications (1x through 4x) are often used to topographically map a specimen (such as a histologically stained thin section) where a wide field of view is desirable in order to quickly note all available specimen features. In many cases, a 2.5x objective may be combined with a wide field eyepiece at 10x magnification to reveal an area having a diameter of 8 millimeters or greater.

For relief contrast observation of living cells, including oocytes, in plastic vessels using transmitted light, these achromat objectives provide excellent field flatness.

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In fact, excessive magnification introduces artifacts, diffraction boundaries, and halos into the image that obscure specimen features and complicate the interpretation of visual observations. Microscope observations are also affected by the sensitivity of the human eye to the intensity and color temperature of the illumination, the age of the observer, the presence of floaters in the eye, and whether the eye is rested or fatigued.

Enabling tissue culture observation through bottles and dishes, these universal semi-apochromat objectives feature a long working distance and high contrast and resolution. Providing flat images and high transmission up to the NIR region, they are well suited for brightfield, DIC, and fluorescence observation.

These semi-apochromat objectives enable phase contrast observation while providing a high level of resolution, contrast, and flatness for unstained specimens.

These super apochromat objectives provide spherical and chromatic aberration compensation and high transmission from the visible to the near infrared. Using silicone oil or water immersion media, which have refractive indexes closely matching that of live cells, they achieve high-resolution imaging deep in living tissue.

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Objectivelens magnification

Unsure of what microscope objective is right for you? Use our guide on selecting the right microscope objective to weigh your options.

Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

These semi-apochromat long-working distance water-dipping objectives for electrophysiology deliver flat images for DIC and fluorescence imaging from the visible range to the near-infrared. Their high NA and low magnification enables bright, precise macro/micro fluorescence imaging for samples such as brain tissue.

For clinical research requiring polarized light microscopy and pathology training, these achromat objectives enable transmitted polarized light observation at an affordable cost.

Low power objectives cover a wide field of view and they are useful for examining large specimens or surveying many smaller specimens.

Designed for low-magnification, macro fluorescence observation, this semi-apochromat objective offers a long working distance, a high NA, and high transmission of 340 nm wavelength light.

where r is the distance separating the two points, λ is the wavelength of illumination, and NAis the objective numerical aperture. In order to magnify the distance until the specimen points appear to the eye at a separation distance of 0.15 millimeters (representing 2 minutes of arc), we examine the relationship:

where M is the optimum microscope magnification. When the illuminating wavelength is assumed to lie in the green region of the visible light spectrum (550 nanometers or 0.00055 millimeters), we can substitute into the equation: