Plano-convex lens

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Biconvexlens

Furthermore, we have observed a distinct type of interaction between PE-Cy5 tandem fluorochromes and the splenocytes of SJL, NOD, and MRL mice: B lymphocytes and some other leukocyte subsets are brightly stained, and Mouse BD Fc Block™ has no significant effect. Therefore, PE-Cy5-conjugated reagents should not be used to stain leukocytes of SJL, NOD, or MRL mice. Reagents conjugated to PE, PerCP, PerCP-Cy5.5, Allophycocyanin (APC), and APC-Cy7 tandem fluorochrome can be used on leukocytes from these mouse strains.

SAv-PE-Cy5 is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. Excitation at 488-nm light leads to a fluorescence emission maximum of ~669 nm.

Double Convex Lenses are made of BK7 or other materials, which are manufactured with an identical convex surface on both sides of the lens. Double Convex lenses have positive focal lengths and form both real and virtual images. Photonchina also provides precision grade Double Convex lenses with tighter manufacturing tolerances.

Double convexlens focal length formula

Double convex lensesexplained

PE-Cy5 tandem fluorochromes have been reported to bind some classes of human macrophages and granulocytes via Fc receptors, and PE has been reported to bind to mouse B lymphocytes via Fc receptors. Preincubation of mouse leukocytes with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142) can reduce the non-specific binding of PE-Cy5-conjugated reagents to mouse B cells. For analysis of human peripheral blood, gating on specific cell populations based upon forward and light scatter, plus lineage markers, can help to overcome this limitation.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Fluorescence emission spectra of R-Phycoerythrin and PE-Cy5. Excitation is at 488 nm and the emission spectra are normalized to the absorbance of both dyes at 488 nm. PE-Cy5 has a peak emission spectrum at 669 nm where there is little overlap with the R-PE spectrum. This reduces compensation required to less than 10%.

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.