I am having trouble getting MM to recognize the basler camera I have attached to my microscope. I currently have BaslerPylon 6.2 installed. I have also generated a help report for why MM is not recognizing my camera and this is the message I receive:

TDI image sensor

Unable to load BaslerPylon library: Failed to load device adapter “BaslerPylon” [ Failed to load module “C:\Program Files\Micro-Manager-2.0\mmgr_dal_BaslerPylon.dll” [ The module, or a module it depends upon, could not be found (Windows error: The specified module could not be found.) ] ]

Where can TDI technology be used? TDI CCDs are used in applications that require the ability to operate in extreme lighting conditions, that require both high speed and high sensitivity, for example: ** inline monitoring, inspection and guidance ** sorting ** earth observation satellite (for weather observation, for example)

Time delay and integrationtdi

Points to note if considering TDI technology TDI requires more care in synchronization and alignment but in general practice these requirements are not difficult to achieve. A TDI sensor can withstand some misalignment (either translational or rotational) without negative effect on image quality and a total misalignment of one pixel or less across the length of a TDI sensor will not affect image quality. In most applications, a 96-stage TDI device can comfortably tolerate a 2-4% velocity mismatch between inspection web and imager. This is not difficult to achieve using a web-mounted encoder to supply a sync signal to the camera, even with webs that change speed and this approach is used in thousands of successful applications. Designed for light-starved applications, TDI typically lacks antiblooming to subdue "glints", and as well, TDI sensors generally use photogates - surface electrodes covering the entire pixel - and while this ensures 100% fill factor, it can attenuate incoming photons in the deep blue region. One camera manufacturer, Teledyne DALSA has produced an anti-blooming device to solve the first problem and has also developed enhanced blue-response TDI sensors to compensate for the latter issue.

Integration timevs exposuretime

I have read that I should be using the latest BaslerPylon software (7.2), however this isn’t an option for me because the scope (for work) is mainly used with another 3rd party’s software that depends upon BaslerPylon 6.2

TDI camera principle

B.t.w., I just checked, and mmgr_dal_BaselPylon.dll is included in the MM version you mention, clearly a dependency is missing).

You will need a version of mmgr_dal_BaslerPylon.dll that has been compiled against BaslerPylon 6.2, and that has the same device interface version as the Micro-Manager Core that you are running (version are available in Help > About Micro-Manager). The best thing would be for you to compile the code yourself (but that needs quite a bit of upfront work).

The easiest way to find out which Pylon version the mmgr_dal_BaslerPylon.dll wants is to use this successor to Dependency Walker or similar: GitHub - lucasg/Dependencies: A rewrite of the old legacy software "depends.exe" in C# for Windows devs to troubleshoot dll load dependencies issues. - If you run DependenciesGui.exe, you can drag and drop the dll onto the window, and you can work out what version of Pylon it expects from the filenames that show up relating to Pylon. For reference, an MM 2.0.0 install that I have wants Pylon 6.2

On a side note: thank you for all of the videos you have recorded through iBiology and such. Your video on how to capture a microscope’s psf with sub-resolution fluorescent beads was the first video I watched that set me down the rabbit hole on scientific imaging.

Time delay and integrationin ccd

What is TDI? TDI refers to Time Delay Integration, a specialised detector readout mode used for observing a high-speed moving object under low light conditions normally undetectable by classic CCD imaging. With an effect similar to that of a linescan camera, TDI is designed to allow continuous movement of the object past the sensor to produce a continuous video image of a moving, two-dimensional object: a definite advantage over stop-then-start capture of traditional technologies. In the past, certain image-capture deployments traded-off sensitivity in order to capture a fast-moving object. TDI-mode technology preserves sensitivity while not degrading image quality, even given the relative fast movement between the sensor and the objects under image. How does TDI work? Based on the concept of accumulating multiple exposures of the same (moving) object, TDI mode effectively increases the integration time available to collect incident light. The technique senses a change in light pattern and shifts them across the CCD in a manner that is synchronised with the movement of the image, in order to integrate more light from the scene. The number of lines on the sensor corresponds to the increase in sensitivity. For example a 256 line TDI sensor provides 256x more sensitivity than a single linescan camera. Imaging in TDI mode provides dramatically increased responsivity compared to other video scanning methods by permitting much greater scanning speeds in low light, or allows reduced lighting levels (and costs) at conventional speeds and so producing a crisp image.

** High quantum efficiency ** High resolution ** Wide imaging areas ** Efficient thermal packaging ** Efficient management of high-speed circuitry

Time delay and integrationmeaning

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Thanks for the response. I will look into compiling the code myself if I continue to have issues. I will have to try again tomorrow when I am at work again ha!

We recently went through this with a Basler camera. The version of Pylon that works for us with a nightly build from last week is Pylon 7.1 (release 7.1.0.25066 is what I have installed)