Laser resonatorworking principle

Photobleaching refers to the loss of fluorescence ability of a fluorophore due to prolonged exposure to light. To avoid photobleaching, researchers should minimize exposure time during imaging.

When considering which filter to use with Cy5, choosing the right filter ensures the best signal-to-noise ratio and contrast. Choosing the right filter can enhance the quality of your fluorescence imaging and improve your experimental results.

Optolong offers filter sets designed specifically for these fluorophores, ensuring high performance and compatibility with existing imaging systems.

Opticalresonatorinlaserpdf

This set provides a high signal-to-noise ratio and is compatible with a variety of imaging equipment. Researchers should also consider the type of optical filters needed, such as bandpass filters or dichroics, to optimize fluorescence imaging.

Optical cavity modes

With the above suggestions, I believe that experimenters can maximize the effectiveness of Cy5 filters in their experiments, ensuring high-quality fluorescence imaging and reliable data.

The Optolong website provides various types of optical filters, including long passes, short pass filters, and multi-bandpass/single-bandpass filters, and supports customized services. Come and contact us for relevant information and get an accurate quotation!

Lasercavity length formula

Experimentation and validation of filter selection ensure optimal performance. Researchers should be open-minded and test different filters to find the best fit for their specific application.

Cy5 is a fluorescent dye that is commonly used in biological and medical research. To be used with Cy5, filters should have specific spectral properties to maximize its fluorescence signal. Ideal filters should include:

Cy5 dye is a bright, far-red fluorescent dye that is excited at 633 nm or 647 nm and is designed for use with the 633 nm or 647 nm laser line. Cy5 has low autofluorescence in biological specimens, making it an ideal choice for fluorescence imaging. Researchers often use Cy5 for applications such as neuronal tracing and cell imaging.

When choosing a filter that is compatible with Cy5, make sure that the filter’s excitation and emission ranges match the spectral properties of Cy5. This can maximize the detection efficiency of the fluorescent signal and improve the accuracy and reproducibility of experimental results.

Laser resonatorPdf

Choosing the right filter requires understanding the specific requirements of Cy5. Researchers must select filters that match the excitation and emission wavelengths of Cy5, which are approximately 649 nm and 670 nm, respectively. Optolong offers specialized filter sets, such as the 30021 Single Band CY5 filter set.

Lasermodes pdf

Several alternatives to Cy5 exist for fluorescence imaging. Alexa Fluor 647 and Allophycocyanin (APC) are popular choices because they have similar spectral properties. These alternatives offer comparable excitation and emission peaks, making them suitable for similar applications.

Confocalresonator

Researchers frequently use Cy5 in fluorescence microscopy, flow cytometry, and other imaging techniques. Common applications include neuronal tracing, cell imaging, and molecular diagnostics.

Selecting the correct Cy5 filter can achieve high-quality fluorescence imaging. Researchers should prioritize filters that match the excitation and emission wavelengths of Cy5. Following best practices, such as proper microscope configuration and regular calibration, can improve experimental results.

Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2024

Cy5 is a bright, far-red fluorescent dye with excitation and emission properties. The excitation peak of Cy5 dye is around 649 nm and the emission peak is near 670 nm. These properties allow Cy5 to produce minimal autofluorescence in biological samples, enhancing image clarity and contrast.

Using anti-fading reagents can help protect Cy5 from photobleaching. Regular inspection and maintenance of equipment ensures optimal performance and longevity of the fluorescent signal.