Infinity-corrected optical system

Presumably, we aren't actually realizing the actual magnification this objective is designed to provide (in fact, its about half what it should be the sensor is 8 mm tall and a ruler placed in the view shows about 1.5 mm across the short dimension of image).

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Can someone clarify what the light path looks like here and how we are actually able to see an image? Is it possible that the image quality is actually really poor, but we just seem to be getting results that are more than suitable for how we are using it?

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All Leica objectives are marked with codes and labels. These identify the objective, its most important optical performance properties, and the main applications it can be used for. For more information, refer to: Labeling of Objectives

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I'm having a hard time wrapping my head around this situation I've come across. I'm essentially recreating a homemade microscope that my understanding would suggest shouldn't work. This setup uses a 10X Olympus PLAN N objective attached to a beam splitter to provide illumination. Then, 55 mm worth of extension tubes connect to a camera (Point Grey Grasshopper3 with a 1/1.2" sensor). There is no tube lens between the infinity corrected objective and the camera sensor (other than the beam splitter).

Infinity objectivemarkers

My understanding of how infinity corrected microscope systems works would seem to suggest that this shouldn't work, but a correctly oriented and seemingly undistorted image shows up on the camera.

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Objectives can be a single lens or mirror, or combinations of several optical elements. They are used in microscopes, binoculars, telescopes, cameras, slide ...

Infinity correctedtube lens

To make it easier for you to find which Leica objectives work best for your microscope and application, you can take advantage of the Objective Finder

It's also possible that you don't have it focussed at exactly it's working distance. Remember the objective is just a (complex) lens. It only produces an image at infinity when the object is at a particular distance. If you put the object further away from the lens the image will be formed nearer than infinity.

Leica achromats are powerful objectives for standard applications in the visual spectral range, offering field flatness (OFN) up to 25 mm. The absolute value of the focus differences between red wavelength and blue wavelength (2 colors) is ≤ 2x depth of field of the objective.

Infinity corrected objectivewithout tube lens

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The objective lens of a microscope forms a magnified, real, intermediate image of the sample or specimen which is then magnified further by the eyepieces or oculars and observed by the user as a virtual image. When a camera is used to observe the sample, then a phototube lens is installed after the objective in addition to, or even in place of, the eyepieces. The phototube lens forms a real image of the sample onto the camera sensor. The objective’s numerical aperture (NA), its ability to gather light, largely determines the microscope’s resolution or resolving power to distinguish fine details of the sample. Also, the working distance, the distance between the sample and objective, and the depth of field, the depth of the space in the field of view within which the sample can be moved without noticeable loss of image sharpness, both greatly depend on the properties of the objective lens. For more information, refer to: Collecting Light: The Importance of Numerical Aperture in Microscopy, How Sharp Images Are Formed, & Optical Microscopes – Some Basics & Labeling of Objectives

Do you need an individual objective for your application? Then contact our Leica OEM Optic Center so that we can offer you a customized solution.

Leica microscope objective lenses are designed and made by our optics specialists to have the highest performance with a minimum of aberrations. The objectives help to deliver superior microscope image quality for many applications, such as life science and materials research, industrial quality control and failure analysis, and medical and surgical imaging.

Infinity corrected objectivemicroscope

Leica semi-apochromats are objectives for applications in the visual spectral range with higher specifications, offering field flatness up to 25 mm. The absolute values of the focus differences for the red wavelength and the blue wavelength to green wavelength (3 colors) are ≤ 2.5x depth of field of the objective.

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Just like any other lens, Objective lens is a lens, without the Tube lens also it will form an image by following the Lens equations, Lens Equations.

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Infinity-correctedobjectivelens

Leica apochromats are objectives for applications with highest specifications in the visual range and beyond, offering field flatness up to 25 mm. The absolute values of the focus differences for the red wavelength and the blue wavelength to green wavelength (3 colors) are ≤ 1.0 x depth of field of the objective.

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It is just that if working distance of the objective lens would be changed, the camera can image at different distances.

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For standard applications, Leica Microsystems offers an extensive range of top-class microscope objectives. There are also Leica objectives which have been optimized for special applications. The highest-performance Leica objectives feature maximum correction and optical efficiency and have won several awards. All over the world, scientists are relying on Leica microscope objectives to gain insights into their area of research.

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The optics of the most basic microscope includes an objective lens and ocular or eyepiece. The objective lens is closest to the sample, specimen, or object being observed with the microscope (see the schematic diagram below). For more information, refer to the article: Optical Microscopes – Some Basics Show schematic diagram