@mani2263 If you have some sort of point sensor you may have different pixel width/height which could be adjusted with Image>Properties (shortcut “P”), see Your screenshot shows 2048 x 1536 pixel which are most probably taken by some CMOS/CCD sensor and it is very unlikely that horizontal and vertical resolution are not the same or at least very similar and a resolution 2412 dpi vs 3175 dpi is implausible. At 3175 dpi 1 pixel equals 8 µm which leads to a 1-pixel-structure of 8 x 8 µm or 64 µm2 which is far away from nanoscale.

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A new product line that I have long awaited. Superb clarity and neutrality, and with a density exactly as advertised (verified with a Sekonic spot meter). There is no slippage/creep of the filter in the slots of my first generation LEE holders (an issue I encountered with glass grads from other suppliers). The included padded pouch is nice, although an exterior label indicating which filter is inside would be a welcome addition, to quickly identify the one you want if you have several different filters. Overall an excellent product, and a distinct step up from the resin equivalents. I hope in the future LEE will expand the range of these filters to include soft gradations, and 1 stop densities.

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Thanks a lot peter, just need 1 more clarification; this info from metadata caters for magnification or we have to apply some scaling factor?. if it is so, then how to apply scaling factor? Thank you

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I have a problem, Could you help? I took photos at x10, and then the photos came with scale bar 500um How can I remove this and calculate the correct measurement?

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So in this case, my image size is 1024 x 1024, I believe, and the zoom is 10x. So then, based on the table, I would enter in the Set Scale window “Distance in pixels: 1” and “Known distance: 1.242” with unit being μm. This should give me the correct scale so I can insert the scale bar?

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It is a very well made component of the Lee Filter System. I bought it for use on my new Fujifilm X100VI. It is a perfect fit and it looks great on my camera. It allows me to use the smaller filters which I had previously from another compact camera system. Overall, very happy with my investment.

Only you–or the microscopist–can know this. The image capture software should put everything it can account for in the metadata, but it’s always possible something else is affecting the scale, like an additional lens in the path to the camera or something. It’s always a good idea to sanity check with something that you know how big it should be. If you do have an extra factor you need to multiple (or divide) the number you enter into the Set scale... dialog so the values represent reality: how many pixels represent a known distance in microns (or whatever unit you’re using)

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The width would be 1392 pixel / 96 dpi (dots per inch = pixel per inch) = 14.5 inch. Be aware that “96 dpi” is a very common standard setting and may be completely wrong, e.g. in pictures from digital consumer cameras.

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Open your image in FIJI, find out the pixel size of the image from the table. Go to Image>Properties and enter the pixel width and pixel height.

It is a very well made component of the Lee Filter System. I bought it for use on my new Fujifilm X100VI. It is a perfect fit and it looks great on my camera. It allows me to use the smaller filters which I had previously from another compact camera system. Overall, very happy with my investment.

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I am new to Fiji, and I’m working with .oib images taken with an Olympus FV1000 confocal microscope. Most images were taken using 10x magnification, and some were taken using 4x. I need to add scale bars to the images, but I’m not sure how to identify what the scale is on the images. I’ve opened Image → Show Info, but I’m not sure how to use this information to set the scale and insert a scale bar. I know that I’m supposed to go to Analyze → Set Scale use the straight line selection tool to make a line selection that corresponds to a known distance, but how do I do this? I’m a bit confused.

@psobolewskiPhD Thanks for your time. I have one more query; what if x and y dimension of the image are not same? As with the image I am sharing info of. I calibrated on X-axis (pixels/um), but when i measured the feature, it was in nano scale which is beyond the resolution of optical microscope. Means there is something wrong, we have to adjust y-axis too. Any idea on that? ImageJ only takes combined x,y-axis calibration in pixels/um. It does’nt treat both axis seperately!

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You would use the procedure you describe when you need to set the scale from the known size of an object in the image and not from prior knowledge.

look at the table of pixel sizes by magnification and field size of your confocal microscope, like for example this one:

Purchased the new adapter to extend my lee 100 system across a new wide angle lens. A superb filter system for all landscape (and other) photography.

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That image info screenshot has everything that you need: You can either use: Resolution: 0.8052 pixels per um Which means in the Set Scale (see below) you enter 0.8052 to Distance in pixels and 1 in Known distance and um in Unit of length Or you can can use the information from: Width: 1271.808 um (1024) you enter 1024 to Distance in pixels and 1271.808 in Known distance and um in Unit of length

Thanks for all the help. I have just one last question: How can I calculate the width and height of an immagine, when the pixel width and height are not shown in the table above? The datapoints of my immagine are these:

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A new product line that I have long awaited. Superb clarity and neutrality, and with a density exactly as advertised (verified with a Sekonic spot meter). There is no slippage/creep of the filter in the slots of my first generation LEE holders (an issue I encountered with glass grads from other suppliers). The included padded pouch is nice, although an exterior label indicating which filter is inside would be a welcome addition, to quickly identify the one you want if you have several different filters. Overall an excellent product, and a distinct step up from the resin equivalents. I hope in the future LEE will expand the range of these filters to include soft gradations, and 1 stop densities.

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Olympus Fluoview-1000 Manual Online: size of pixels, Table. 5 Pixel Size (Μm) Versus Box Size And Magnification At Zoom = 1. The Magnification And Pixel Size Of An Image Is A Function Of Lens Magnification, Box Size And Zoom Factor. However, These...

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