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Meem, M. et al. Inverse-designed achromatic flat lens enabling imaging across the visible and near-infrared with diameter > 3 mm and NA=0.3. Appl. Phys. Lett. 117, 041101 (2020).

National Laboratory of Solid State Microstructures, Key Laboratory of Intelligent Optical Sensing and Manipulations, Jiangsu Key Laboratory of Artificial Functional Materials, College of Engineering and Applied Sciences, Nanjing University, Nanjing, 210093, China

Fan, Z. B. et al. A broadband achromatic metalens array for integral imaging in the visible. Light Sci. Appl. 8, 67 (2019).

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Equation (4) is a general form to describe the relation between the phase distortion Δφ(ρ, ω) and focus performance for any type of achromatic flat lenses. To quantify the restriction relation, we mainly consider AMDLs in the following, of which the phase profile can be derived based on accumulation of optical path36,

Ocular lensmagnification

Although the analysis of coherence can predict the max Jω(F) of an AMDL with specific parameters, it is hard to derive the corresponding optimal height distribution h(ρ) directly from Eqs. (4–7). Furthermore, considering the feasibility in fabrication, the aspect ratio of h(ρ) needs to be maintained at a low level in general. The maximum H in this work is up to 15 μm, and width of ring Δ is set as 2 μm to satisfy Nyquist−Shannon sampling theorem (Δ ≤ λ/2NA). Thus, the aspect ratio may reach 7.5:1, which is very challenge for the grayscale laser lithography technique working in the visible.

Peng, Y. F. et al. The diffractive Achromat full spectrum computational imaging with diffractive optics. ACM Trans. Graph. 35, 31 (2016).

Scanningobjective magnification

Your objective lens isn't just for increasing the size of your subject; it can also provide better resolution. For example, achromatic lenses contain two smaller lenses (convex and concave) that are used to limit the refracting light of your subject, and phase-contrast lenses use phase plates to pick up miniscule changes in wavelength amplitude, making moving subjects easier to observe. Lenses like these help reduce ghost images so that the real image is projected to your eyepiece.

Lim, S. W. D., Meretska, M. L. & Capasso, F. A high aspect ratio inverse-designed holey metalens. Nano Lett. 21, 8642–8649 (2021).

Wang, P., Mohammad, N. & Menon, R. Chromatic-aberration-corrected diffractive lenses for ultra-broadband focusing. Sci. Rep. 6, 21545 (2016).

The general form of group delay ∂φ/∂ω cannot be derived in this case, which means it becomes nearly impossible to work out a quantitative restriction relation based on previous method. To explore the impact of phase distortion, we elaborately deal with this problem from another perspective – the coherence of the light field. The coherence of the light field has been extensively studied in wave optics44,45. Achromatic flat lens needs to work within a range of frequencies, so we pay more attention to the coherence in the frequency domain. With analogy to the mutual coherence function in classical optics45, the frequency domain mutual coherence function of wave distortion at two coordinates ρ1 and ρ2 on the exit pupil (coincide with the lens surface in the single-lens imaging system), denoted as Jω(ρ1,ρ2), can be defined as

where h(ρ) and n(ω) are radial height distribution (ranging from 0 to maximum thickness H) and refractive index, respectively. Firstly, to reveal the restriction of diameter and thickness on the upper bound of coherence at exit pupil (denoted as max Re Jω(ρ1,ρ2)) for AMDLs, we perform calculation on 5 different cases based on Eqs. (3) and (6). In each case the AMDL has the same NA (equal to 0.1), working spectrum (400–1100 nm) and is made of the same material (AZ4562, the refractive index is provided in Supplementary S3-1). Figure 1b shows the max Re Jω(ρ1,ρ2) of the first three cases (denoted as S1-S3) with H equal to 15 μm and diameters (denoted as D) ranging from 1 mm to 10 mm. Figure 1c shows max Re Jω(ρ1,ρ2) of last three cases (denoted as S3-S5) with D equal to 10 mm and H ranging from 15 to 1 μm. The performance trend is clearly revealed in these results. For a thicker and smaller AMDL (D = 1 mm, H = 15 μm), high coherence region (red area where max Re Jω(ρ1,ρ2) ≈1) covers a large area in (ρ1, ρ2) space. As the thickness decreases or diameter increases, the red area shrinks gradually, which indicates the overall coherence of corresponding AMDL will decrease. Secondly, the upper bound of Jω(F) with respect to diameter and thickness is calculated based on Eq. (4), with the same fixed NA, working spectrum, and material, as is shown in Fig. 1d. It is clear that max Jω(F) has a positive relation to thickness and negative relation to diameter. The max Jω(F) in case S1-S5 is 0.72, 0.43, 0.21, 0.11 and 0.03, respectively, which is marked as five-pointed star in Fig. 1d. More details of the calculation are provided in Supplementary S1-5.

Meem, M. et al. Imaging from the visible to the longwave infrared wavelengths via an inverse-designed flat lens. Opt. Express 29, 20715–20723 (2021).

AmScope PA10X 10X Plan Achromatic Microscope Objective : Amazon.ca: Industrial & Scientific.

High powerobjective magnification

AMDL samples S1-S5 are fabricated by using the grayscale laser lithography technique (see more details in “Methods”). The top view and side view of a typical AMDL are shown in Fig. 3a. Figure 3b particularly shows the photograph of sample S3 and its zoom-in details, whose height profile is characterized by the step profiler with comparison to the theoretical design, as shown in Fig. 3c. The average fabrication error is <150 nm over the whole lens (<1%), showing extremely good agreement. Details of other samples are provided in Supplementary S3-2.

Swanson, G. J. Binary Optics Technology: The Theory and Design of Multi-level Diffractive Optical Elements. (Massachusetts Institute of Technology, Lincoln Laboratory, 1989).

Chen, W. T. et al. A broadband achromatic metalens for focusing and imaging in the visible. Nat. Nanotechnol. 13, 220–226 (2018).

Objective magnificationexample

where Eff is focus efficiency defined as the ratio of the optical power within 3 times the full-width at half-maximums (FWHMs) of the point spread function (PSF) on the focal plane over the whole transmitted optical power on the exit pupil47, and wmax is the maximum normalized diameter of PSF in the whole spectrum. In fact, Jω(ρ1,ρ2) cannot reach maximum for every two points at exit pupil for a non-ideal lens, and thus maximum focus efficiency will inevitably degrade according to Eqs. (4–5), which indicates performance degradation from an ideal lens to a non-ideal lens is actually due to the reduction in the degree of coherence.

NLGI Grade 2 - Silicone-based grease with a base oil viscosity of 76 cSt at 40°C, lithium-thickened, with no solid lubricants.

Meem, M., Majumder, A. & Menon, R. Full-color video and still imaging using two flat lenses. Opt. Express 26, 26866–26871 (2018).

By now, we are aware of the importance of the thickness in AMDL design, which is also in coincidence with the thicker achromatic metalens design for improved performance28. Although AMDL can reach larger thickness than metalens, there is still a great distance to achieve the level required by an ideal macro-size lens, due to the extremely difficulty in manufacturing. Our experimental results highlight the significance of our theory and method that provide a guidance to derive the optimal thickness for an AMDL with given diameter and NA. In addition, it should be mentioned that the working bandwidth Δλ is also an important parameter in the design of AMDLs. However, the restriction relation between Δλ and other parameters is not clear in analytical form Eq. (7) due to the approximations applied in the derivation of this equation (see Supplementary Material S1-6). In fact, if the thickness H, diameter D and NA of an AMDL are fixed, the focus performance will be improved as working bandwidth Δλ decreases through numerical simulation based on Eq. (4), which is shown in Supplementary S2-6. A centimeter-scale AMDL with larger NA (≥0.3), narrower working bandwidth (450 nm–680 nm) and relatively good performance is also revealed theoretically and provided in Supplementary S2-6. To be noted, further improvement on comprehensive performance can be made by incorporating AMDL with matured imaging process algorithms. As a preliminary attempt, we reconstruct high-contrast images from the raw images taken by AMDLs based on a simple built-in image processing algorithm in our cell phone, and find the maximum improvement in images taken by S3, which is provided in Supplementary S3-9. These results also highlight the necessity of achromatic design and reservation of details in raw images. It is very hopeful that the performance of the AMDL can be further enhanced to meet the real application by incorporating with advanced imaging processing, like the deep learning52, etc.

Chen, W. T. et al. A broadband achromatic polarization-insensitive metalens consisting of anisotropic nanostructures. Nat. Commun. 10, 355 (2019).

FLIR Technical Support Center · Free Extended Warranty · Repair and Calibration Services · Infrared Training Center · The World's Sixth Sense ...

To overcome these difficulties, an optimization framework is developed as shown in Fig. 2a, which includes three main steps. The first is Search, which can yield the optimal height distribution by combining genetic algorithm (GA) and Hook-Jeeves algorithm (HJA)48,49,50 to avoid local minima and speed up the convergence as well. The second is Smooth, which can remove the high aspect-ratio structures in the lens. However, Smooth will inevitably degrade the performance of the lens. Therefore, we applied the third step – Gradient to optimize the samples once more51. The design goal is set as maximizing Jω(F). Through this optimization framework, we successfully derived the quasi-optimum height distribution of five samples for case S1-S5, with aspect ratio of most rings less than 2:1. Figure 2b shows Jω(F) over the course of the optimization process for sample S3. Figure 2c shows the details around the sharp dip in Fig. 2b, which is caused by the Smooth operation. Figure 2d shows the height profile of samples S1-S5 after optimization. We also calculated the diffracted fields along the propagation axis z for these samples based on Rayleigh-Sommerfeld diffraction theory to confirm the achromatic properties46. Figure 2e shows the diffracted fields of S3 at 14 wavelengths between 400 and 1100 nm. It is clear that light focuses at the same point (z = 50.9 mm) for all the wavelengths. More details about optimization process and theoretical results of other samples are provided in Supplementary S2.

where F is the focus length, ri is the distance from ρi to the focus (i = 1,2), R is the radius of the lens and dΣ1dΣ2 is a surface element of exit pupil Σ of lens45. The achromatic focus performance of the lens relies on the value of Jω(F). Generally speaking, Jω(F) can be explained as the relative light intensity at focus compared to the background noise on focus plane over the whole working spectrum, and there exists a connection between upper bound of Jω(F) and the maximal averaging focus efficiency based on scalar diffraction theory46 (see Supplementary S1–4 for more details),

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a Photographs of fabricated samples S3, the left one and right one are top and side views, respectively. b Local view of the whole AMDL (left) and zoom-in optical image (mid and right). Scale bar, 100 μm. c The designed (black line) and measured (red line) height distribution, respectively

Objective magnificationin microscope

where nmax is the maximum n(ω) over the whole spectrum and Dmax is the maximum diameter for given H, NA, nmax, and max Jω(F). Although it is just a rough approximation, Eq. (7) gives us an intuitive insight on the designing trade-offs for AMDL. Increase of the diameter of an AMDL with fixed NA requires an increase in the thickness, otherwise it leads to degradation in the focus performance. It is worth noting that though Eq. (7) is derived for AMDL, it also works for achromatic metalenses approximately, by which the comprehensive performance of an achromatic flat lens can be defined using an index of product \(P = D(1 - \sqrt {1 - J_\omega (F)} )NA\). It has taken the size, NA, focus performance into account at the same time. The comprehensive performance of some of reported AMDLs and achromatic metalens with respect to the effective thickness Heff = (nmax −1)H are summarized in Fig. 1e. Although most achromatic metalenses have shown good performance in achromatic focusing, it is clear that the small lens size (that leads to small field of view) limits the comprehensive performance, which is in fact determined by their low effective thickness. In contrast, by increasing the thickness, the AMDLs show much better comprehensive performances (i.e., an order of magnitude higher than achromatic metalenses). More details of theoretical analysis are provided in Supplementary S1-6 and Table S1.

The objective lens, on the other hand, looms over your subject, typically near the middle of the microscope. This is because the objective lens is responsible for gathering light reflections from your subject. It then shoots a beam of light into the microscope, which becomes an image that you observe from the eyepiece containing the ocular lens.

Thompson, A. R., Moran, J. M. & Swenson, G. W. Jr Interferometry and Synthesis in Radio. Astronomy. 3rd edn., 781–786. Springer, Cham, 2017).

AmScope exclusive ALL-IN-ONE 3D DIGITAL INSPECTION MICROSCOPE. View different angles and perspectives of objects with ease.

Zheng, H. Y. et al. Large-scale metasurfaces based on grayscale nanosphere lithography. ACS Photonics 8, 1824–1831 (2021).

More advantages in imaging performance of the AMDL are further evaluated by comparing with a conventional refractive lens (LE1234, denoted as RL). Figure 5c shows the images of Nanjing University logo (16 mm × 20 mm, generated by a projector) taken from sample S3 and RL. Although the image taken from sample S3 is a little bit darker due to relatively lower efficiency, there is no color blurred effect, which is very obvious in the case for RL (see the corresponding zoom-in edge images of both). Figure 5d shows the imaging results of R, G, B letters (generated by a projector), where all three letters can be clearly imaged on the same plane for AMDL. However, the image of R and G will be blurred when B is clearly imaged for RL due to the chromatic aberration (see the corresponding zoom-in edge images at the upper-left corner). Considering its thin and lightweight, such an AMDL is very promising for portable and convenient applications in the nearly future.

This is why a microscope is such a good investment for anyone interested in science. If you want to understand and examine the world around you, there's no better tool. AmScope's selection is built to last, and we carry all kinds of objective lenses as well, so a microscope from us will serve you well for many years.

a The schematic of the optimization framework. b The change of Jω(F) over the course of the optimization process. Before the sharp dip is the Search operation, where GA and HJA are applied in turn. c The details around the sharp dip in b. The sharp dip is caused by the Smooth operation. d Height distribution (2D top view) of samples S1-S5. e Calculated light intensity profiles along propagation axis for sample S3 at 14 wavelengths

where ω, F, ρ and c are the angular frequency, focal length, radial coordinate, and speed of light in vacuum, respectively. The group delay ∂φ/∂ω can be derived from Eq. (1) and then restriction relations between the size, thickness, working spectrum and numerical aperture (NA) of an ideal achromatic flat lens can be derived19. However, when it comes to the non-ideal achromatic flat lens, there will exist distortion Δφ(ρ, ω) in the phase profile as

Figuring out the total magnification power of your microscope is easy: just multiply the power of your objective lens by your ocular lens. For instance, if your eyepiece has 10x magnification and you're using a low-power lens (10x), you have 100x magnification in total. Switch to your scanning lens (4x), and magnification becomes 40x. It's important to keep in mind that the ocular lens and objective lens total magnification is ultimately what you're viewing. If you were viewing your subject through a single lens, then that lens would have to be extremely powerful to match what you can easily get with both. Therefore, one lens isn't nearly as effective without the other.

Kress, B. C. & Meyrueis, P. Applied Digital Optics: From Micro-Optics to Nanophotonics. pp. 521–579. (Wiley, West Sussex, 2009).

Shkondin, E. et al. Fabrication of high aspect ratio TiO2 and Al2O3 nanogratings by atomic layer deposition. J. Vac. Sci. Technol. A 34, 031605 (2016).

where 〈〉ω means frequency-averaging operation. The real part of Jω indicates the degree of the coherence at ρ1 and ρ2. If there is no distortion in the whole working spectrum (or equivalently distortion is a constant over the whole radial axis), the real part of Jω will reach the maximum value +1, which in fact means the sub-waves emitted from ρ1 and ρ2 to the focus (denoted as E(ρ1) and E(ρ2)) are totally constructive. If the distortion is large, the real part of Jω will be closed to the minimum value −1, which means E(ρ1) and E(ρ2) are totally destructive, as is shown in Fig. 1a. Based on propagation of mutual intensity, the normalized coherence function at focus Jω(F) can be derived as

Conventional refractive lens has achieved great success in the area of imaging, metrology, detection, etc1,2, but its bulky and heavy characteristics prevent the systems from being compact, lightweight, and miniaturized, which is in high demand for portable and convenient applications3. Metalenses, made of subwavelength nano-unit cells with ultrathin, lightweight, and flat architecture, do provide possible solutions that to some extent overcome the shortcomings of conventional diffractive lens with higher focusing efficiency and weak shadow-effect4,5,6,7,8,9. However, metalenses still severely suffer from poor imaging quality under broadband illumination due to serious chromatic aberration10,11,12,13. Several pioneering works have demonstrated achromatic metalens operating over a broad wavelength range14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31. However, according to the fundamental limitations of the achromatic flat lens, the diameter of an ideal achromatic metalens (under the diffraction limit) is restricted by its thickness (the max height each nano-unit can achieve)18,19,20,32,33,34. As results, the diameters of reported achromatic metalenses with NA ≥ 0.1 in the visible do not exceed 200 μm due to their relatively small thicknesses (<4 μm) up to date. Such miniaturized metalenses are useful in the micro-nano optics or integrated optics (for example, endoscope7). However, millimeter- or centimeter-scale metalenses are necessary when it comes to other fields, such as augmented reality, lens modules in cell phones, landscape cameras and telescopes1,15. Under theoretical estimation, hundred-micron thickness is needed to access the ideal centimeter scale (5~10 mm) achromatic metalens, which requires a very large aspect ratio (>1000:1) for the constructing nano-units, while the reported largest one is only about 50:128,34,35. Therefore, to access an ideal large-scale achromatic metalens based on the common meta-designs seems to be unachievably difficult.

The focusing performance of AMDLs was experimentally examined by a home-built optical setup, where a super-continuum laser (Fianium, Super-continuum 4 W, the spectrum is provided in Supplementary S3-5) with a beam expander was used to generate large area uniform beam (with diameter > 1 cm). Figure 4a shows the measured cross-section of intensity profiles at 14 different wavelengths for sample S3 with a designed focal length f = 50.9 mm, from which it is observed that the brightest spots are almost located at the designed position (white dash line) for all wavelengths. The measured 2D (top) and 1D (bottom) point spread function (PSF) at each wavelength at the focal plane (dashed line in Fig. 4a) are displayed in Fig. 4b with the intensity normalized to 0~1, showing good coincidence with theoretical Gaussian distribution and confirming the broadband achromatism very well. Figure 4c shows the experimentally measured focal lengths of samples S1-S5, where the maximum discrepancy is found less than 2%. The experimentally measured FWHM of the PSF at each wavelength are plotted in the Fig. 4d, which is slightly larger than the diffraction limit (λ/2NA, black dashed lines). Figure 4e shows the measured focus efficiency of S1-S5 at each wavelength. The definition of focus efficiency is the same as the one used in analyzation above. The average efficiency is about 68%, 42%, 31%, 18%, 5% for samples S1-S5, respectively. Based on Eq. (5), we can estimate the coherence Jω(F) of samples S1-S5, which is 0.61, 0.34, 0.18, 0.085, 0.014, respectively, in good coincidence with our theoretical prediction (Fig. 1d). The focus performance can be further quantified by measuring Strehl ratios, as are shown in Fig. 4f. The Strehl ratio is calculated based on the measured 2D PSF at each wavelength43. The average Strehl ratio of sample S1 is 0.7252, which is close to the diffraction limit (~0.8), while that of S5 is only 0.4545. More details about focus performance of other samples are provided in Supplementary S3-6.

Chen, M. K. et al. Edge detection with meta-lens: from one dimension to three dimensions. Nanophotonics 10, 3709–3715 (2021).

Everyone knows that microscopes are a crucial tool in science, but few realize how versatile and adaptable they can be. Thanks to the variance in lenses, microscopes can serve all kinds of purposes for all kinds of people, from the doctor identifying cancer cells to the child wanting to get a closer look at their favorite bug. Once you know how all of the optical elements work together, like the ocular lens vs objective lens, it's easy to maximize the efficiency of your microscope.

In this work, we try to solve the tough problem in two steps. First, we carefully derived the fundamental limits on the comprehensive performance (include size, NA and focus efficiency) of a non-ideal AMDL with limited thickness based on the coherence of the light field. Second, we propose an optimization framework to design feasible large-scale AMDLs with performance closed to the upper bound. We substantially validate these results by designing and characterizing five AMDLs of millimeter to centimeter sizes. The optical characterization results prove the effectiveness of our theory and design methods, and demonstrated a relatively high performance of large-scale flat lens in achromatic imaging. The advancement is further confirmed by comparing it with a conventional refractive lens, indicating a major step towards the practical applications.

Totalmagnification

Sun, P. et al. Broadband achromatic polarization insensitive metalens over 950 nm bandwidth in the visible and near-infrared. Chin. Opt. Lett. 20, 013601 (2022).

There are many other kinds of objective lenses out there, so you have no shortage of options. Do some research and find out which lens best suits your needs and goals.

The authors acknowledge the financial support from The National Key R&D Program of China (2022YFA1404300, 2017YFA0303701), National Natural Science Foundation of China (Nos. 91850204, 12174186, 62288101). Tao Li thanks the support from Dengfeng Project B of Nanjing University. Guixin Li thanks the supports from Guangdong Provincial Innovation and Entrepreneurship Project (2017ZT07C071) and Natural Science Foundation of Shenzhen Innovation Commission (JCYJ20200109140808088).

a Image results of 1951 USAF resolution test chart taken from samples S1 ~ S5. The whole images captured by CCD (left column). Scale bar, 250 μm; zoom-in images of group 5 element 1~3 (middle column); the light intensity (denoted as I) along the yellow dashed lines (right column). b The broadband MTF of samples S1-S5 and diffraction limited MTF (black dashed line). c Image of Nanjing University logo taken from sample S3 and a refractive lens. Zoom-in image shows the edge details of the logo. Scale bars, 2 mm. d Images of three colorful letters (N, J, U) taken from sample S3 and a refractive lens. Zoom-in image shows the edge details of the letter R. Scale bars, 4 mm

Doskolovich, L. L. et al. Design of diffractive lenses operating at several wavelengths. Opt. Express 28, 11705–11720 (2020).

Afterwards, the comprehensive imaging performance of these AMDLs are systematically analyzed. First, the 1951 United State Air Force (USAF) resolution test chart was used as imaging target, illuminated by a broadband illumination source (HL100S, color temperature = 3200 K), and a charge coupled device (CCD, Blackfly S BFS-U3-200S6C) was employed to capture the image. The left column in Fig. 5a shows the whole images taken from S1-S5 captured by the CCD under white light illumination. In each figure, the closed blue dashed lines denote the field of view (FOV) and the red dashed lines denote the images of group 5 element 1~3. It is clear that due to the achromatic design, there exists no color blur effect in all the images taken by AMDLs, which is obvious in the cases of chromatic refractive lens and Fresnel lens (shown in Supplementary S3-7). The middle column in Fig. 5a displays the details of group 5 element 1~3, whose intensity along the yellow dashed lines is shown in the right column in Fig. 5a. Using Imax and Imin to denote the maximum and minimum intensity along the yellow dashed lines, the image contrast, defined as (Imax – Imin)/(Imax + Imin), is 0.66, 0.44, 0.34, 0.15, 0.06 for images taken by S1 to S5, respectively. It is clear that the image contrast has a positive relation with the coherence Jω(F). These results indicate that Jω(F) cannot be too low (<0.1), otherwise the corresponding image contrast will be extremely low and even unable to resolve the image details (e.g., sample S5). We further characterized the broadband Modulation Transfer Function (MTF) under white light illuminance of each sample, as the results shown in Fig. 5b. It shows very high value of S1 (red dashed line) that is close to the diffraction-limited one (black dashed line) while the MTF of S5 (orange dashed line) is far below it. Although the results show that S1 owns the best focus performance, it is restricted by the relatively small FOV preventing it from widely practical applications. In fact, considering the comprehensive performance, sample S3 would be the best one among samples S1-S5 which has already been indicated in Fig. 1e.

Fortunately, the multi-level diffractive lens provides an alternative strategy to reach the achromatism by locally controlling the height of each diffractive ring36,37,38,39,40,41,42,43. The reported maximum thickness of an achromatic multi-level diffractive lens (AMDL) working in the visible can reach as high as 10 μm, which ensures a relatively large diameter (~1 mm) of the lens43. However, such a large thickness is realized with sacrifice in very small numerical aperture (NA = 0.028) corresponding to a large ring width (8 μm), where the aspect ratios of rings can be maintained at a low level. Nevertheless, such a thickness is still far from the one that an ideal centimeter-scale achromatic lens requires. A possible solution would lie in a compromise in designing a non-ideal broadband achromatic lens with an achievable thickness and fabrication feasibility. From this point of view, elaborate optimizations in light field coherence at focus and detailed structures are very important and necessary.

Often, your microscope will have at least three objective lenses on a rotating disc, each with a different magnification level. If you find your current lens lacking, it's easy to switch to one of the others. Objective lenses with higher magnification have shorter focal lengths, or less space between the lens and the surface of the subject. Since depth of field decreases as magnification increases, those wanting a broader field of view should stick to shorter lenses. For example, if your current objective lens has 100x magnification but you need a wider field of view, you'll want to switch to a lens with lower magnification, such as 40x.

Xiao, X., Zhao, Y., Ye, X. et al. Large-scale achromatic flat lens by light frequency-domain coherence optimization. Light Sci Appl 11, 323 (2022). https://doi.org/10.1038/s41377-022-01024-y

Jan 10, 2024 — Behavior shaping is a cornerstone of Applied Behavior Analysis. It's a technique that uses small, manageable steps to teach new skills or ...

A thin positive photoresist (AZ4562) layer with thickness of ~400 nm was spin-coated onto a silicon substrate. Then, the wavelength dependent (400–1100 nm) refractive index was measured by using the spectroscopic ellipsometer.

Park, J. S. et al. All-glass, large metalens at visible wavelength using deep-ultraviolet projection lithography. Nano Lett. 19, 8673–8682 (2019).

Hold up to the white light one of the filters cyan, magenta or yellow. What do you notice about the color of the light? White light contains all of the colors ...

Wang, Y. J. et al. High-efficiency broadband achromatic metalens for near-IR biological imaging window. Nat. Commun. 12, 5560 (2021).

Khorasaninejad, M. et al. Metalenses at visible wavelengths: diffraction-limited focusing and subwavelength resolution imaging. Science 352, 1190–1194 (2016).

Xu, B. B. et al. Metalens-integrated compact imaging devices for wide-field microscopy. Adv. Photonics 2, 066004 (2020).

Flat lenses, including metalens and diffractive lens, have attracted increasing attention due to their ability to miniaturize the imaging devices. However, realizing a large scale achromatic flat lens with high performance still remains a big challenge. Here, we developed a new framework in designing achromatic multi-level diffractive lenses by light coherence optimization, which enables the implementation of large-scale flat lenses under non-ideal conditions. As results, a series achromatic polymer lenses with diameter from 1 to 10 mm are successfully designed and fabricated. The subsequent optical characterizations substantially validate our theoretical framework and show relatively good performance of the centimeter-scale achromatic multi-level diffractive lenses with a super broad bandwidth in optical wavelengths (400–1100 nm). After comparing with conventional refractive lens, this achromatic lens shows significant advantages in white-light imaging performance, implying a new strategy in developing practical planar optical devices.

Objective magnificationformula

Sun, T. et al. Polarization-insensitive achromatic metalens based on computational wavefront coding. Opt. Express 29, 31902–31914 (2021).

Aieta, F. et al. Multiwavelength achromatic metasurfaces by dispersive phase compensation. Science 347, 1342–1345 (2015).

In contrast, your microscope's eyepiece will usually have only one ocular lens, though you can usually swap the eyepiece as well. The standard magnification level of the ocular lens is 10x, but there are stronger ones available. When selecting an eyepiece, you should think about eye relief, or the required distance between your eyes and the lens. Eyepieces with large eye relief give you some space, while those with small eye relief require you to be up close.

Previous works have analyzed the fundamental limitations on an ideal achromatic flat lens based on the assumption that the phase profile φ provided by lens following the hyperbolic relation20,32,

The microscope is one of the most iconic and commonly used tools in many scientific fields. We rely on these devices to observe things that are so small that they are otherwise invisible to the naked eye. To do this, the microscope makes use of both an ocular and an objective lens. If you don't know the difference, don't worry; this article will tell you everything you need to know about these two lens types and how they function together to make microscopes work.

by SA Kovalenko · 1999 · Cited by 695 — Pump--supercontinuum-probe (PSCP) spectroscopy with femtosecond time resolution is developed theoretically and experimentally.

a Experimental light intensity profiles of sample S3 at 14 different incident wavelengths. Scale bars, 15 μm. b Normalized intensity profiles along the white dashed lines of a. Scale bars, 10 μm. c Experimentally measured focal length shifts of samples S1-S5. d Experimentally measured FWHM of PSF of samples S1-S5 and the diffraction limited FWHM (black dashed dots). e Measured focus efficiencies of samples S1-S5. f Experimentally measured Strehl ratios of samples S1-S5

Born, M. & Wolf, E. Principles of Optics: Electromagnetic Theory of Propagation, Interference and Diffraction of Light. 7th edn., 554–632. (Cambridge University Press, Cambridge, 2013).

Low powerobjective magnification

The objective and ocular lens are found on different parts of the microscope. The ocular lens is part of the eyepiece and therefore closer to your eye as you look into the microscope. The location of the eyepiece always indicates the correct observing position at or near the top of the microscope.

by G Fava · 1983 · Cited by 23 — Lasers in Surgery and Medicine is the official journal of the American Society for Laser Medicine & Surgery publishing studies on medical and surgical use ...

While it may initially seem redundant to have two separate lenses in your microscope, they do far more together than they ever could on their own.

Fan, Y. L. et al. Experimental demonstration of genetic algorithm based Metalens design for generating side-lobe-suppressed, large depth-of-focus light sheet. Laser Photonics Rev. 16, 2100425 (2022).

A thin positive photoresist (AZ4562) layer with a thickness of ~25 µm was spin-coated onto a 1.1 mm thick glass substrate. The photoresist was baked at 100 °C for 5 min. The grayscale laser lithography technique was used to transfer the grayscale information of the AMDL to the spatially dependent exposure on the photoresist layer. The wavelength of the laser used for grayscale lithography is 405 nm. After developing the exposed photoresist layer, the AMDL with pre-defined three-dimensional morphology were realized on glass substrate.

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T.L. and X.X. developed the idea. X.X. proposed the theoretical design and performed the numerical simulation; X.L., Y.R., J.D., and G.L. fabricated and characterized the samples; X.X. performed the optical measurement with the help of Y.Z., X.Y., and C.C; T.L. organized the project. X.X. and T.L. analyzed the results with support from all authors. All authors discussed the results. X.X. and T.L. wrote the manuscript with input from all authors.

In conclusion, we elaborately derived the fundamental limitations existed in non-ideal AMDLs by analyzing the coherence of the light field, and developed an optimization framework to realize a large-scale AMDL with relatively high performance, large thickness and small aspect ratio of ring structures. As a proof of concept, a series of AMDLs were designed and demonstrated in super-broad wavelength range (400~1100 nm), among which the maximum diameter and thickness of AMDLs reach 1 cm and 15 μm, respectively. Considering the size, efficiency and numerical aperture, such an AMDL presented an extremely high comprehensive performance, while no existing achromatic flat lens is reported approaching a comparable value like this (Fig. 1e). Our strategy is helpful to evaluate the upper bound of an achromatic flat lens to applicable level with the progress of manufacturing, which is very promising to be applied to the conventional imaging systems.

There are four main types of objective lenses, each with a different diameter of field of view, and therefore a different magnification level:

Arbabi, E. et al. Controlling the sign of chromatic dispersion in diffractive optics with dielectric metasurfaces. Optica 4, 625–632 (2017).

A comprehensive evaluation on AMDL can be derived if the constructive red area is chosen as the only domain of integration in Eq. (4),

a Schematics of the coherence of wave distortion in MDL. Ef is the superposition of E(ρ1) and E(ρ2) at focus. The constructive Ef and destructive Ef are denoted as blue dashed lines and red dashed lines, respectively. b The distribution of max Jω(ρ1,ρ2) with thickness fixed at 15 μm and diameter equal to 1, 3, 10 mm, respectively. c The distribution of max Jω(ρ1,ρ2) with diameter fixed at 10 mm and thickness equal to 15, 5, 1 μm, respectively. d The max Jω(F) with respect to different samples in dimensions of diameter and thickness. e Comprehensive performance P of reported achromatic metalenses and AMDLs with respect to effective thickness Heff

Fiber optic lights are energy-efficient, as they use a single LED light source to illuminate multiple fibers. This low power consumption makes them an eco- ...