We illustrate this idea in Fig. 4a, which depicts an ordinary grating beam deflector alongside two beam deflectors with engineered dispersion: a superchromatic (exceeding grating dispersion) beam deflector and a beam deflector exhibiting positive dispersion. In the grating beam deflector, the OGL from O to the wavefront, represented by the dashed black line, increases linearly with increasing distance u along the wavefront, i.e., the upper ray has a larger OGL than the lower ray. With a change in frequency, different points along the wavefront acquire phase shifts that are proportional to their OGLs and linear in u, resulting in a wavefront tilt. The magnitude and direction of this tilt can be controlled by modifying the difference in the OGLs for different points along the wavefront. Extending and shortening the paths of the upper and lower rays, respectively, increases their OGL difference, thereby increasing the rate of wavefront tilt with respect to frequency, producing a superchromatic beam deflector. This is depicted schematically in the superchromatic design in Fig. 4a. By contrast, shortening the upper ray’s path and extending that of the lower ray to the point that the OGL of the lower ray exceeds that of the upper ray, as shown in the positive-dispersion design in Fig. 4a, inverts the direction of the wavefront tilt. Such a beam deflector will exhibit positive dispersion, similar to refractive prisms made of materials with normal dispersion.

The simulated optical intensity distributions presented in Figs. 2c, 3d, e and Supplementary Figs. S1–4 and the simulated intensity distributions for the fabricated beam deflectors that are shown in Supplementary Fig. S6 were obtained by modelling the metasurfaces as ideal phase masks that do not modify the local optical intensity. The propagation of the optical waves in the regions before, between, and after the metasurfaces was performed using the plane wave expansion technique, which is a full-wave vectorial approach that does not involve any approximations2. The incident waves in Fig. 2c and Supplementary Fig. S6 were normally incident Gaussian beams with a waist radius of 130 μm whose waist was in the plane of the first metasurface. The incident waves for the results shown in Fig. 3d, e and Supplementary Figs. S1–3 were normally incident plane waves; for the results in Supplementary Fig. S4, plane waves with the indicated incidence angles were used. The simulated deflection angles shown in Figs. 2d and 5b correspond to the angles of the peak intensity in the far-field patterns of the deflected beams. The variations in the focal lengths with the wavelength that are presented in Fig. 3c for the twisted metalens and the annular single-layer metalens show the corresponding displacements of the peaks of the simulated intensity distributions presented in Fig. 3d, e and Supplementary Fig. S2 as functions of wavelength.

Campbell, S. D. et al. Review of numerical optimization techniques for meta-device design [Invited]. Opt. Mater. Express 9, 1842–1863 (2019).

where c is the speed of light in vacuum, lm is the path length between surfaces m−1 and m inside a material with refractive index nm, and φm is the phase imparted by the mth surface. If the mth surface does not include a metasurface, then φm = 0. Because the system focuses the rays at I, all rays’ paths acquire the same Φ (ref. 2).

Kruk, S. & Kivshar, Y. Functional meta-optics and nanophotonics governed by Mie resonances. ACS Photonics 4, 2638–2649 (2017).

Three critical design characteristics of the objective set the ultimate resolution limit of the microscope. These include the wavelength of light used to illuminate the specimen, the angular aperture of the light cone captured by the objective, and the refractive index in the object space between the objective front lens and the specimen.

This work was supported by the DARPA Extreme Optics and Imaging programme and was performed in part at the Center for Nanoscale Systems (CNS) at Harvard University, a member of the National Nanotechnology Coordinated Infrastructure Network (NNCI), which is supported by the National Science Foundation under NSF award no. 1541959. Additional device fabrication was performed in the Conte Nanotechnology Cleanroom at the University of Massachusetts Amherst.

Born, M. & Wolf, E. Principles of Optics: Electromagnetic Theory of Propagation, Interference and Diffraction of Light. 7th edn (Cambridge University Press, Cambridge, 1999).

The main data supporting the findings of this study are available within the article and its Supplementary Information. Additional data are available from the corresponding author upon reasonable request.

In the wave optics picture, the first metasurface twists the incident light, generating a helical beam with a large (N = 750) orbital angular momentum, and the second metasurface removes the wavefront’s helicity and focuses the beam to the point I. Different radial portions of the finely structured helical beam traverse the substrate at different group velocities via an effect similar to one that has been recently observed35. Portions of the wave closer to the optical axis have slower axial group velocities. A similar group velocity dependence could be achieved if the substrate had a radially dependent refractive index; thus, the combination of the homogeneous substrate and the two metasurfaces exhibits behaviour similar to that of a graded index lens.

To demonstrate the concept and design methodology, we designed, fabricated, and characterized achromatic, superchromatic, and positive-dispersion beam deflectors that have 0, 3, and −1 times the dispersion, respectively, of an ordinary grating. The beam deflectors are composed of two cascaded metasurfaces (as shown in Figs. 2a and 4a) and are designed to deflect a normally incident 850-nm beam to an angle of 20°. The phase profiles of the metasurfaces were obtained by imposing appropriate constraints on the rays’ OGLs (Supplementary Information) and are presented in Supplementary Fig. S5. Simulated intensity distributions for each design are presented in Supplementary Fig. S6. A single-layer metasurface deflector was also designed to serve as a control.

Department of Electrical and Computer Engineering, University of Massachusetts Amherst, 151 Holdsworth Way, Amherst, MA, 01003, USA

Chromatic dispersion spatially separates white light into colours, producing rainbows and similar effects. Detrimental to imaging but essential to spectroscopy, chromatic dispersion is the result of material properties in refractive optics and is considered an inherent characteristic of diffractive devices such as gratings and flat lenses. Here, we present a fundamental relation connecting an optical system’s dispersion to the trajectories light takes through it and show that arbitrary control over dispersion may be achieved by prescribing specific trajectories, even in diffractive systems. Using cascaded metasurfaces (2D arrays of sub-micron scatterers) to direct light along predetermined trajectories, we present an achromatic twisted metalens and experimentally demonstrate beam deflectors with arbitrary dispersion. This new insight and design approach usher in a new class of optical systems with wide-ranging applications.

The last, but perhaps most important, factor in determining the resolution of an objective is the angular aperture, which has a practical upper limit of about 72 degrees (with a sine value of 0.95). When combined with refractive index, the product:

One of the most significant advances in objective design during recent years is the improvement in antireflection coating technology, which helps to reduce unwanted reflections that occur when light passes through a lens system. Each uncoated air-glass interface can reflect between four and five percent of an incident light beam normal to the surface, resulting in a transmission value of 95-96 percent at normal incidence. Application of a quarter-wavelength thick antireflection coating having the appropriate refractive index can increase this value by three to four percent. Nikon's more recent CFI Plan Apochromat Lambda Series of objective lenses utilize their proprietary Nano Crystal Coat technology, which consists of several layers of ultra-low refractive index nano-sized crystals. As objectives become more sophisticated with an ever-increasing number of lens elements, the need to eliminate internal reflections grows correspondingly. Some modern objective lenses with a high degree of correction can contain as many as 15 lens elements having many air-glass interfaces. If the lenses were uncoated, the reflection losses of axial rays alone would drop transmittance values to around 50 percent. The single-layer lens coatings once utilized to reduce glare and improve transmission have now been supplanted by multilayer coatings that produce transmission values exceeding 99.9 percent in the visible spectral range.

Moreover, although the twisted metalens performs well for fields at normal incidence, aberrations are apparent at angles of incidence as small as 0.2° (see Supplementary Fig. S4). While these aberrations are problematic for imaging, the twisted metalens could, for example, be used as a broadband collimator or focuser in applications for which a wide field of view is unnecessary. The correction of monochromatic aberrations has previously been demonstrated26, and achromatic metalenses with large fields of view may be possible in systems with three or more layers.

If the frequency is changed to \(\omega + \Delta \omega\), the fiducial ray will acquire a different total phase of \(\Phi + \Delta \Phi\). We will designate as ‘achromatic’ a system in which the entire set of rays over a continuous frequency range near ω, to the first-order approximation in \(\Delta \omega\), is focused to I. The phase change \(\Delta \Phi\) can be partitioned into two contributions. First, the frequency change will cause the ray to acquire a different total phase along its original trajectory. Second, each surface will deflect the ray by a different angle, altering its trajectory. The contribution of this second term is zero (see the Supplementary Information), so the system is achromatic if and only if \(\Delta \Phi = l_{\mathrm g}\Delta \omega /c\) is the same for all rays, where

As light rays pass through an objective, they are restricted by the rear aperture or exit pupil of the objective, as illustrated in Figure 4. The diameter of this aperture varies between 12 millimeters for low magnification objectives down to around 5 millimeters for the highest power apochromatic objectives. Aperture size is extremely critical for epi-illumination applications that rely on the objective to act as both an imaging system and condenser, where the exit pupil also becomes an entrance pupil. The image of the light source must completely fill the objective rear aperture to produce even illumination across the viewfield. If the light source image is smaller than the aperture, the viewfield will experience vignetting from uneven illumination. On the other hand, if the light source image is larger than the rear aperture, some light does not enter the objective and the intensity of illumination is reduced.

The axial range through which an objective can be focused without any appreciable change in image sharpness is referred to as the depth of field. This value varies radically from low to high numerical aperture objectives, usually decreasing with increasing numerical aperture (see Table 2 and Figure 2). At high numerical apertures, the depth of field is determined primarily by wave optics, while at lower numerical apertures, the geometrical optical "circle of confusion" dominates. The total depth of field is given by the sum of the wave and geometrical optical depths of field as:

Oskooi, A. F. et al. Meep: a flexible free-software package for electromagnetic simulations by the FDTD method. Comput. Phys. Commun. 181, 687–702 (2010).

Liu, V. & Fan, S. H. S4: a free electromagnetic solver for layered periodic structures. Comput. Phys. Commun. 183, 2233–2244 (2012).

Whatdoesthestage do ona microscope

Simulated focal length shifts for the bilayer twisted metalens and a single-layer metalens are shown in Fig. 3c. As expected, the twisted metalens is achromatic (i.e., \({\mathrm d}f/{\mathrm d}\lambda = 0\)) at its design wavelength. To verify that the achromatic response is not merely due to the annular aperture, a single-layer metalens with the same annular aperture was also designed for comparison. The focal-plane intensity distributions of the twisted and annular single-layer metalenses are shown in Fig. 3d, e. Intensity distributions for the twisted metalens over a wider range of wavelengths and corresponding distributions for a single-layer metalens with a circular aperture are presented in Supplementary Fig. S2.

World-class Nikon objectives, including renowned CFI60 infinity optics, deliver brilliant images of breathtaking sharpness and clarity, from ultra-low to the highest magnifications.

Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

Ocular lensmicroscope

Starting from first principles, we present a fundamental relation between the ray trajectories in optical systems and their chromatic response. Contrary to the prevalent description of chromatic dispersion that attributes it to optical elements such as gratings or metalenses, the new relation reveals variations in ray trajectories as the origin of chromatic dispersion. This relation offers clear insight into the chromatic response of optical systems and establishes a framework for designing unconventional optical systems with arbitrary chromatic dispersions. Specifically, we show that an optical system is achromatic if all ray trajectories have equal optical group lengths (OGLs). Therefore, the design of achromatic systems involves selecting a set of ray trajectories with equal OGLs and then designing components (e.g., metasurfaces) to direct light along those trajectories.

The measured deflection efficiencies, where this efficiency is defined as the ratio of the power of the deflected beam to that of the incident beam, are shown in Fig. 5c. All devices show an efficiency peak between 900 and 975 nm. These peaks are red-shifted and show reduced efficiency compared with what modelling predicts (Supplementary Figs. S9 and S10). This discrepancy is likely the result of fabrication imperfections. Compared to a single-surface deflector, a cascaded system is expected to show reduced transmission efficiency, as the losses at each individual surface accumulate. The metasurface design could be further optimized to achieve higher efficiency36.

a Schematic of an achromatic bilayer metasurface beam deflector. b Phase profiles of the metasurfaces composing the beam deflector shown in a. c Full-wave simulation results for the deflection of a Gaussian beam by the beam deflector. d Wavelength dependence of the deflection angle for the achromatic bilayer beam deflector and a single-layer metasurface beam deflector.

Optical Advantages: Aspheric lenses are designed to reduce aberrations, especially spherical aberration. Spherical aberration is a distortion that occurs when ...

Presented in Figure 1 is a cut-away diagram of a microscope objective being illuminated by a simple two-lens Abbe condenser. Light passing through the condenser is organized into a cone of illumination that emanates onto the specimen and is then transmitted into the objective front lens element as a reversed cone. The size and shape of the illumination cone is a function of the combined numerical apertures of the objective and condenser. The objective angular aperture is denoted by the Greek letter θ and will be discussed in detail below.

A.M. and A.A. conceived the research. A.A. simulated and designed the devices, and A.M. performed full-wave simulations and analysed the simulation data. A.M. and M.M. fabricated the samples, and A.M. and A.A. performed the measurements and data analysis. A.M. and A.A. prepared the manuscript with input from all authors. Supplementary information accompanies the manuscript on the Light: Science & Applications website.

MicroscopeObjectives magnification

Rays of angular frequency ω (blue lines) travel from object point O through a system of M surfaces to image point I. The system is achromatic if rays of a different angular frequency (red lines) leaving O also converge at I. Purple interfaces represent metasurfaces, and black interfaces represent boundaries between materials of different refractive indices.

Pahlevaninezhad, H. et al. Nano-optic endoscope for high-resolution optical coherence tomography in vivo. Nat. Photonics 12, 540–547 (2018).

Chen, W. T. et al. A broadband achromatic polarization-insensitive metalens consisting of anisotropic nanostructures. Nat. Commun. 10, 355 (2019).

Stagemicroscope

For many years, objective lenses designed for biological applications from most manufacturers all conformed to an international standard of parfocal distance. Thus, a majority of objectives had a parfocal distance of 45.0 millimeters and were considered interchangeable. With the migration to infinity-corrected tube lengths, a new set of design criteria emerged to correct for aberrations in the objective and tube lenses. Coupled to an increased demand for greater flexibility to accommodate the need for ever-greater working distances with higher numerical apertures and field sizes, interchangeability between objective lenses from different manufacturers disappeared. This transition is exemplified by the modern Nikon CFI-60 optical system that features "Chrome Free" objectives, tube lenses, and eyepieces. Each component in the CFI-60 system is separately corrected without one being utilized to achieve correction for another. The tube length is set to infinity (parallel light path) using a tube lens, and the parfocal distance has been increased to 60 millimeters. Even the objective mounting thread size has been altered from 20.32 to 25 millimeters to meet new requirements of the optical system.

a Schematic of the measurement setup. The sample is illuminated by a tuneable light source, and the deflected beam is imaged on a camera. b Deflection angles for the control, achromatic, positive-dispersion, and superchromatic beam deflectors as functions of wavelength. The solid curves represent the simulated responses. c Deflection efficiencies of the beam deflectors as functions of wavelength. The solid lines serve as guides for the eye. The power measurements were obtained at the locations indicated in a.

Arbabi, E. et al. Controlling the sign of chromatic dispersion in diffractive optics with dielectric metasurfaces. Optica 4, 625–632 (2017).

Figure 3a shows an example of a bilayer metalens that achieves equal OGLs by deflecting the rays to different angles along the azimuthal direction. Incident rays closer to the optical axis are deflected by larger azimuthal angles by the first metasurface to compensate for the shorter length they travel between the second metasurface and the focal point I. As a proof of concept, we have designed a circularly symmetric bilayer metalens with an annular aperture (150 μm < r1 < 300 μm), selecting \(\varphi _1 = N\theta + f_1\left( r \right)\) and \(\varphi _2 = - N\theta + f_2\left( r \right) + \omega /c\sqrt {r^2 + f^2}\) for the two metasurfaces, which are separated by a 500-μm-thick substrate with a refractive index of 1.46. We chose N = 750 and a focal length of f = 1 mm; \(f_1(r)\) and \(f_2(r)\) were obtained by setting lg = 1785 μm for all ray paths and are shown in Fig. 3b. Two-dimensional phase profiles for the metasurfaces are presented in Supplementary Fig. S1.

where λ is the wavelength of illumination, n is the refractive index of the imaging medium, NA is the objective numerical aperture, M is the objective lateral magnification, and e is the smallest distance that can be resolved by a detector that is placed in the image plane of the objective. Notice that the diffraction-limited depth of field (the first term on the right-hand side of the equation) shrinks inversely with the square of the numerical aperture, while the lateral limit of resolution is reduced with the first power of the numerical aperture. The result is that axial resolution and the thickness of optical sections are affected by the system numerical aperture much more than is the lateral resolution of the microscope (see Table 2).

We begin by describing the achromatic condition and then discuss its extension to arbitrary chromatic dispersions. Consider the optical system shown in Fig. 1, which is constructed of different transparent media. The surfaces separating the media may be non-planar and may include a metasurface with a phase profile φm. Suppose that the system is designed to bring a set of rays of angular frequency ω originating at an object point O into focus at an image point I (as shown in Fig. 1). The total phase accumulated by a fiducial ray in this set can be expressed as

The same setup was used to measure the deflection efficiency. A calibrated power meter (PM100D with an S120C head, Thorlabs) was placed at the positions indicated in Fig. 5a. The power meter was moved to follow the deflected beam as the wavelength of the incident light was tuned. The efficiencies reported in Fig. 5c are the deflected-to-incident power ratios. The reflectivity at near-normal incidence of the unpatterned region was measured to be 94 ± 2% across the 700–1000 nm band. This is predominantly due to the gold mirror but also includes reflection from the air–SU-8 interface.

The phase profiles for the achromatic beam deflector (Fig. 2b), achromatic metalens (Fig. 3b), apochromatic metalens (Supplementary Fig. S3b), and fabricated beam deflectors (Supplementary Fig. S5) were obtained by first determining the ray paths necessary to achieve the desired dispersion (see the Supplementary Information) and then numerically computing the phase surfaces to direct rays along these paths. As shown in the Supplementary Information, two solutions exist for each prescribed dispersion: one branch was chosen for the achromatic and positive beam deflectors, and the other was chosen for the superchromatic beam deflector. This is illustrated in the ray diagrams in Supplementary Fig. S5, in which rays travel from left to right (right to left) for the achromatic and positive-dispersion (superchromatic) beam deflector(s). The choice of solution is also distinguished by the sign of the phase gradient for the first metasurface, \({\mathrm d}\phi _1/{\mathrm d}x\). The solutions were chosen on the basis of the second-order dispersion coefficients.

First, a 485-nm-thick layer of amorphous silicon (α-Si) was deposited on one side of a 1-mm-thick fused silica substrate using plasma-enhanced chemical vapour deposition. An approximately 200-nm-thick layer of positive electron beam resist (ZEP 520A-7, Zeon Chemicals) was spin-coated on top of the α-Si layer, followed by a 70-nm-thick layer of conductive polymer (AR-PC 5090, Allresist), which was applied to mitigate charging during electron beam lithography. The beam deflector patterns were exposed using a 125 keV electron beam lithography system (ELS-F125, Elionix). The water-soluble conductive polymer was removed, and the resist was developed in a developer (ZED-N50, Zeon Chemicals). A hard mask was created by first evaporating approximately 50 nm of aluminium oxide onto the resist and then lifting it off in a solvent. The pattern was then transferred to the α-Si layer by means of plasma etching in C4F8 and SF6 chemistry. The hard aluminium oxide mask was removed in a mixture of ammonium hydroxide and hydrogen peroxide heated to 80 °C. Figure 4c shows a scanning electron micrograph of the sample at this stage. The nano-posts were encapsulated in an approximately 5-μm-thick layer of SU-8 photoresist. The SU-8 was spin-coated, baked at 95 °C to reflow, exposed, and cured to form a permanent protective layer over the nano-posts. On the opposite side of the substrate, a similar layer of SU-8 was deposited to act as an adhesion layer. We then evaporated approximately 50 nm of gold onto this surface to form the folding mirror.

The group delay imposed by a single-layer dispersive metasurface \(\left( {\varphi{\prime} \ne 0} \right)\) has recently been engineered to modify the chromatic response of high-contrast transmitarray8 and Pancharatnam–Berry11,12,13,14 metasurfaces. Because the delay is proportional to the quality factors of the meta-atoms8, the achievable group delays are limited, restricting the applicability of this approach for chromatic correction to narrow bandwidths, very small metasurfaces, or both. However, dispersion can also be controlled using the first term in Eq. (2), which is related to the geometric lengths over which a ray travels between surfaces. In systems of non-dispersive metasurfaces \(\left( {\varphi {\prime} = 0} \right)\), an achromatic response is achieved by imposing the requirement of a constant \(l_{\mathrm g} = \mathop {\sum }\nolimits_{m = 1}^{M + 1} n_{m_{\mathrm{g}}}l_{m}\) for all rays. Therefore, an achromatic optical system can be designed by first identifying a set of ray paths that satisfy the equal-OGL condition and then directing the rays along these paths using metasurfaces. We illustrate this novel approach by discussing the design of an achromatic beam deflector and an achromatic metalens doublet.

The advent of highly efficient metasurfaces has spurred significant renewed interest in engineering chromatic dispersion5,6,7,8,9,10,11,12,13,14. Optical metasurfaces are two-dimensional (2D) arrays of scatterers (or meta-atoms) that shape optical wavefronts with subwavelength resolution. They can replace conventional elements15,16,17,18,19,20, facilitate the implementation of novel functionalities by offering unprecedented control over the flow of light21,22,23,24,25, and enable planar optical systems that can be mass produced similarly to semiconductor chips26,27,28,29. Like diffractive elements, metasurfaces have wavelength-independent phase profiles due to phase wrapping7, and the conventional approach of chromatic correction through cascading has been proven ineffective in focusing systems made only of such elements30. New approaches that permit operation at a few discrete wavelengths have been proposed6,7,31, but such approaches are not viable in wideband applications requiring correction over a continuous spectral range (i.e., achromatic components). Consequently, achromatic systems employing metasurfaces have been limited to diffractive-refractive hybrids9,32 or small metasurfaces that exploit meta-atom dispersion8,11,12,13,14. Metasurfaces function as correctors in hybrid diffractive-refractive systems, reducing the overall size of the system, but the refractive components are the main focusing elements in such systems. Metasurfaces relying on meta-atom dispersion to correct chromatic aberrations offer the desired planar form factor, but their size and numerical aperture are inherently limited by the highest meta-atom quality factors that can reliably be attained8.

The simple relation between the change in the accumulated phase and the OGL (i.e., \(\Delta\Phi = l_{\mathrm g}\Delta \omega /c\)) allows dispersion to be engineered by imposing different constraints on the OGL, thereby producing metasystems that exhibit dispersive behaviour other than achromatic dispersion or simple grating dispersion. When the frequency changes by \(\Delta \omega\), the phase at each point on the wavefront changes by \(\Delta \Phi = l_{\mathrm g}\Delta \omega /c\). If the OGLs of rays travelling from O to different points on the wavefront are all the same, then the phase changes at all points are equal: the wavefront is unchanged, and the system is achromatic. On the other hand, the variation of the wavefront with the wavelength can be engineered as desired by selecting ray trajectories that have prescribed OGLs and then designing metasurfaces to direct rays along these trajectories.

The numerical aperture of an optical system is a dimensionless number that characterizes the range of angles over which the system can accept or emit light.

where R is the separation distance, λ is the illumination wavelength, n is the imaging medium refractive index, and θ is one-half of the objective angular aperture. In examining the equation, it becomes apparent that resolution is directly proportional to the illumination wavelength. The human eye responds to the wavelength region between 400 and 700 nanometers, which represents the visible light spectrum that is utilized for a majority of microscope observations. Resolution is also dependent upon the refractive index of the imaging medium and the objective angular aperture. Objectives are designed to image specimens either with air or a medium of higher refractive index between the front lens and the specimen. The field of view is often quite limited, and the front lens element of the objective is placed close to the specimen with which it must lie in optical contact. A gain in resolution by a factor of approximately 1.5 is attained when immersion oil is substituted for air as the imaging medium.

McClung, A., Mansouree, M. & Arbabi, A. At-will chromatic dispersion by prescribing light trajectories with cascaded metasurfaces. Light Sci Appl 9, 93 (2020). https://doi.org/10.1038/s41377-020-0335-7

Table 1 lists working distance and numerical aperture as a function of magnification for the four most common classes of objectives: achromats, plan achromats, plan fluorites, and plan apochromats. Note that dry objectives all have a numerical aperture value of less than 1.0 and only objectives designed for liquid immersion media have a numerical aperture that exceeds this value.

To design the meta-atoms for these metasurfaces, we first obtained the complex transmission amplitude t(w) as a function of the post width w, which was determined using a rigorous coupled-wave analysis solver37. We chose a lattice constant of a = 300 nm, which satisfies the Nyquist sampling criterion \(a < \lambda /\left( {n_1 + n_2} \right)\) (ref. 38) for arbitrary incidence and deflection angles for refractive indices of \(n_1 = 1.45\) and \(n_2 = 1\) and at all wavelengths λ within the range considered. The amplitude and phase of t(w) as functions of the post width are shown in Supplementary Fig. S7a. The optimal post width \(w(\varphi )\) for a given transmission phase φ was chosen by maximizing \({\mathrm{Re}}\{ t(w){\mathrm e}^{i\varphi }\}\). These optimal widths are shown as a function of the phase in Supplementary Fig. S7b. In these simulations, we used refractive indices of n = 1.45, 1.56, and 3.84 for fused silica, SU-8, and α-Si, respectively. The input metasurface of each beam deflector had dimensions of 500 μm × 500 μm; the dimensions of the output metasurface were selected to fully contain the output beam for a wavelength range of 700–1000 nm and were obtained from the simulations presented in Supplementary Fig. S6. The output metasurface dimensions for the beam deflectors were also 500 μm × 500 μm.

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Giovannini, D. et al. Spatially structured photons that travel in free space slower than the speed of light. Science 347, 857–860 (2015).

Lukosz, W. Optical systems with resolving powers exceeding the classical limit. II. J. Optical Soc. Am. 57, 932–941 (1967).

Parts ofa microscope

by GS Jung · 2020 · Cited by 10 — Field curvature is the sum of the target lens and liquid lens. Therefore, the field curvature of the liquid lens can affect the measurement of ...

Arbabi, E. et al. Multiwavelength polarization-insensitive lenses based on dielectric metasurfaces with meta-molecules. Optica 3, 628–633 (2016).

When a manufacturer's set of matched objectives, e.g. all achromatic objectives of various magnifications (a single subset of the objectives listed in Table 1), are mounted on the nosepiece, they are usually designed to project an image to approximately the same plane in the body tube. Thus, changing objectives by rotating the nosepiece usually requires only minimal use of the fine adjustment knob to re-establish sharp focus. Such a set of objectives is described as being parfocal, a useful convenience and safety feature. Matched sets of objectives are also designed to be parcentric, so that a specimen centered in the field of view for one objective remains centered when the nosepiece is rotated to bring another objective into use.

Astilean, S. et al. High-efficiency subwavelength diffractive element patterned in a high-refractive-index material for 633 nm. Opt. Lett. 23, 552–554 (1998).

During assembly of the objective, lenses are first strategically spaced and lap-seated into cell mounts, then packaged into a central sleeve cylinder that is mounted internally within the objective barrel. Individual lenses are seated against a brass shoulder mount with the lens spinning in a precise lathe chuck, followed by burnishing with a thin rim of metal that locks the lens (or lens group) into place. Spherical aberration is corrected by selecting the optimum set of spacers to fit between the lower two lens mounts (the hemispherical and meniscus lens). The objective is parfocalized by translating the entire lens cluster upward or downward within the sleeve with locking nuts so that objectives housed on a multiple nosepiece can be interchanged without losing focus. Adjustment for coma is accomplished with three centering screws that can optimize the position of internal lens groups with respect to the optical axis of the objective.

Aieta, F. et al. Multiwavelength achromatic metasurfaces by dispersive phase compensation. Science 347, 1342–1345 (2015).

Mar 28, 2021 — Telescope Specifications Change with Diameter and f/5 focal ratio. ... Telescopes with longer focal lengths will have higher magnification and ...

Colburn, S., Zhan, A. & Majumdar, A. Metasurface optics for full-color computational imaging. Sci. Adv. 4, eaar2114 (2018).

The field diameter in an optical microscope is expressed by the field-of-view number or simply field number, which is the diameter of the viewfield expressed in millimeters and measured at the intermediate image plane. The field diameter in the object (specimen) plane becomes the field number divided by the magnification of the objective. Although the field number is often limited by the magnification and diameter of the ocular (eyepiece) field diaphragm, there is clearly a limit that is also imposed by the design of the objective. In early microscope objectives, the maximum usable field diameter was limited to about 18 millimeters (or considerably less for high magnification eyepieces), but modern plan apochromats and other specialized flat-field objectives often have a usable field that can range between 22 and 28 millimeters or more when combined with wide-field eyepieces. Unfortunately, the maximum useful field number is not generally engraved on the objective barrel and is also not commonly listed in microscope catalogs.

is known as the numerical aperture (abbreviated NA), and provides a convenient indicator of the resolution for any particular objective. Numerical aperture is generally the most important design criteria (other than magnification) to consider when selecting a microscope objective. Values range from 0.1 for very low magnification objectives (1x to 4x) to as much as 1.6 for high-performance objectives utilizing specialized immersion oils. As numerical aperture values increase for a series of objectives of the same magnification, we generally observe a greater light-gathering ability and increase in resolution. The microscopist should carefully choose the numerical aperture of an objective to match the magnification produced in the final image. Under the best circumstances, detail that is just resolved should be enlarged sufficiently to be viewed with comfort, but not to the point that empty magnification hampers observation of fine specimen detail.

What is the objective in a microscopeexplain

In a system comprising many metasurfaces, light of different wavelengths can propagate along significantly different paths. In this article, we derive a relation connecting the paths through an optical system along which light of a specific wavelength travels to the system’s chromatic behaviour. This relation allows us to concisely state the achromaticity criterion for focusing systems: in an achromatic system, the OGLs of all ray paths are equal. We propose a design framework for achromatic optics based on predefined trajectories and demonstrate its application with two examples. We extend this design framework to systems with arbitrary dispersion and validate it experimentally by demonstrating superchromatic and achromatic beam deflectors and a beam deflector exhibiting positive dispersion.

Magnesium fluoride is one of many materials utilized in thin-layer optical antireflection coatings, but most microscope manufacturers now produce their own proprietary formulations. The general result is a dramatic improvement in contrast and transmission of visible wavelengths with a concurrent destructive interference in harmonically-related frequencies lying outside the transmission band. These specialized coatings can be easily damaged by mis-handling and the microscopist should be aware of this vulnerability. Multilayer antireflection coatings have a slightly greenish tint, as opposed to the purplish tint of single-layer coatings, an observation that can be employed to distinguish between coatings. The surface layer of antireflection coatings used on internal lenses is often much softer than corresponding coatings designed to protect external lens surfaces. Great care should be taken when cleaning optical surfaces that have been coated with thin films, especially if the microscope has been disassembled and the internal lens elements are subject to scrutiny.

Resolution for a diffraction-limited optical microscope can be described as the minimum detectable distance between two closely spaced specimen points:

Figure 4b shows the materials and geometry used to implement the beam deflectors. Instead of placing the metasurfaces on opposite sides of a substrate, we employed a folded metasystem architecture27 (Fig. 4b, left): light deflected by the first metasurface propagates to the opposite side of the substrate and is reflected towards the second metasurface by a gold mirror. This layout allows both metasurfaces to be located on the same side of the substrate, simplifying fabrication. The metasurfaces were implemented using a high-contrast transmitarray platform21. Each metasurface is composed of an array of amorphous silicon nano-posts with square cross-sections and equal heights (Fig. 4b). The nano-posts rest on a fused silica substrate and are encapsulated from above with SU-8 polymer. Curves relating the transmission phase to the post width are presented in Supplementary Fig. S7; see ‘Materials and methods’ for the details of the metasurface design.

a Comparison of a single-layer metasurface grating beam deflector with bilayer superchromatic (s.c.) and positive-dispersion designs. b Schematics showing (left) the cascaded metasystem, which uses a gold mirror on the back of the fused silica substrate to ‘fold’ the cascaded systems depicted in a; a portion of one of the metasurfaces (centre); and a unit cell (right) showing a single meta-atom. c A photograph (left) showing the beam deflector patterns and their reflections, an optical microscope image (centre) of the folded achromatic beam deflector and an SEM image (right) of the nano-posts.

Neder, V. et al. Combined metagratings for efficient broad-angle scattering metasurface. ACS Photonics 6, 1010–1017 (2019).

Our measurement setup is shown schematically in Fig. 5a. Our tuneable light source comprised a supercontinuum light source (YSL SC-5-FC) and a monochromator (Acton Research Corporation SpectraPro 2150i) coupled to a single-mode fibre. The source exhibited a full-width at half-maximum of approximately 0.7 nm in the 700–1000 nm spectral range. Normalized spectra at representative wavelengths are shown in Supplementary Fig. S8. The light exiting the fibre was gently focused to produce a spot size of approximately 150 μm at the sample, which was located approximately 25 cm from the lens. The beam deflector sample was affixed to a mirror mount attached to a three-axis micropositioning stage. This allowed us to position the incident beam to impinge on a specific metasurface at normal incidence. A camera (Edmund Optics EO-5012M) was mounted on a rotation stage with the rotation axis centred on the sample. As the deflected beam approached the limits of the image sensor, a reference image was taken, and the camera was repositioned, allowing image fields acquired at different positions to be stitched together. The deflection angles were determined by calculating the intensity centre of mass for these images. Sample images are shown in Supplementary Fig. S11.

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The focal length of a lens system is defined as the distance from the lens center to a point where parallel rays are focused on the optical axis (often termed the principal focal point). An imaginary plane perpendicular to the principal focal point is called the focal plane of the lens system. Every lens has two principal focal points for light entering each side, one in front and one at the rear. By convention, the objective focal plane that is nearer to the front lens element is known as the front focal plane and the focal plane located behind the objective is termed the rear focal plane (see Figure 4). The actual position of the rear focal plane varies with objective construction, but is generally situated somewhere inside the objective barrel for high magnification objectives. Objectives of lower magnification often have a rear focal plane that is exterior to the barrel, located in the thread area or within the microscope nosepiece.

Beam deflectors are the basic building blocks of gradient metasurfaces, and such metasurfaces can be considered beam deflectors with spatially varying deflection angles. A single-layer, non-dispersive metasurface beam deflector that deflects normally incident light by an angle θ exhibits a grating dispersion of \({\mathrm d}\theta /{\mathrm d}\lambda = {\mathrm{tan}}\theta /\lambda\) (ref. 2). As shown in Fig. 2a, a metasurface beam deflector can be realized using two parallel non-dispersive \(\left( {\varphi {\prime} = 0} \right)\) metasurfaces by choosing points O and I far from the metasurfaces along the normal direction and at an angle θ with respect to the normal direction, respectively. To achieve achromatic beam deflection (i.e., \({\mathrm d}\theta /{\mathrm d}\lambda = 0\)), the two metasurfaces should be designed such that the OGL from O to I, or equivalently (as shown in Fig. 2a) \(l_{\mathrm g} = n_{\mathrm g}l_{AB} + l_{BC}\), is the same for all ray paths (ng: group index of the material separating the metasurfaces). Constraining lg to a fixed value determines the path taken by a ray incident at any point A on the first metasurface. Rays incident at different points on this surface must be deflected by different angles to meet the imposed constraint. Using the deflection angles of these paths and the grating equation, the phase profiles are obtained (see the Supplementary Information). Figure 2b shows the phase profiles for a 20° bilayer metasurface beam deflector produced via this procedure. Simulated intensity distributions depicting the deflection of Gaussian beams with different wavelengths are shown in Fig. 2c, and the wavelength dependence of the deflection angles for the bilayer and single-layer beam deflectors is shown in Fig. 2d, demonstrating the achromatic response of the bilayer deflector at its design wavelength of 550 nm.

Now, we consider the achromatic on-axis focusing of a normally incident beam by cascaded non-dispersive metasurfaces. According to the equal-OGL condition, if all of the metasurfaces have paraxial regions, then a set of cascaded parallel metasurfaces cannot be achromatic because the axial ray has the minimum OGL. However, an achromatic cascaded metalens with an annular aperture can be realized if the metasurfaces are designed to deflect the rays along paths of equal OGLs. Unlike the group delay approach described above, annular apertures impose no restrictions on the lens size and hence enable a larger space–bandwidth product33,34, the ultimate determinant of the information content of an image.

A new technique that uses two-dimensional (2D) metasurfaces to control the dispersion of light in optical systems could pave the way for new devices for a wide range of optical applications. Chromatic dispersion occurs when the different wavelengths of a light beam arrive at their destination at different times. Although useful in applications like spectroscopy, chromatic dispersion must be compensated for in wideband imaging systems. Now, a team of researchers led by Amir Arbabi from the University of Massachusetts Amherst in the United States has developed a new technique that uses 2D arrays of sub-micron scale metasurfaces that shape the optical wavefronts of light with subwavelength resolution. By directing the light along predetermined trajectories, the technique allows chromatic dispersion to be controlled and could be used in applications from photography and astronomy to microscopy.

To assess the performance of the beam deflectors, we first performed finite-difference time-domain simulations39 of the single-surface beam deflector. An illustration of the simulation geometry and representative cross-sectional fields are shown in Supplementary Fig. S9. In each simulation, the structure was excited by a normally incident monochromatic plane wave originating in the SU-8. The fields at the output planes were projected onto ideal fields, and the efficiency was calculated by dividing the power in each projected field by the incident power. Both x- and y-polarized incident fields were simulated. The simulated efficiencies are shown alongside the measured data in Supplementary Fig. S10.

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To characterize the dispersion, we illuminated the input metasurface of each beam deflector with an unpolarized tuneable light source and imaged the deflected beams on a camera. A schematic depicting the measurement setup is shown in Fig. 5a. Representative spectral data for the tuneable light source are shown in Supplementary Fig. S8. The measured deflection angles for light with wavelengths between 750 and 1000 nm in steps of 25 nm are shown in Fig. 5b along with the expected angles found through simulation (see ‘Materials and methods’). The measured deflection angles for all devices show good agreement with the simulated angles.

Just as the brightness of illumination in a microscope is governed by the square of the working numerical aperture of the condenser, the brightness of an image produced by the objective is determined by the square of its numerical aperture. In addition, objective magnification also plays a role in determining image brightness, which is inversely proportional to the square of the lateral magnification. The square of the numerical aperture/magnification ratio expresses the light-gathering power of the objective when utilized with transmitted illumination. Because high numerical aperture objectives are often better corrected for aberration, they also collect more light and produce a brighter, more corrected image that is highly resolved. It should be noted that image brightness decreases rapidly as the magnification increases. In cases where the light level is a limiting factor, choose an objective with the highest numerical aperture, but having the lowest magnification factor capable of producing adequate resolution.

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Buralli, D. A. & Rogers, J. R. Some fundamental limitations of achromatic holographic systems. J. Optical Soc. Am. A 6, 1863–1868 (1989).

The twisted metalens serves as an illustration of the unconventional elements enabled by our approach, but better-performing designs are possible. To demonstrate, we have also designed a bilayer apochromatic metalens with the same aperture and focal length as the twisted metalens. This apochromat, shown in Fig. 3f, uses only radial deflections, producing a rotationally symmetric design. Similar to the twisted metalens, the apochromat was designed by selecting ray paths with equal OGLs. The operational principle can be qualitatively understood by considering the paths of two rays: The ray that enters the system farthest from the optical axis experiences the largest deflection, thus achieving the longest OGL within the substrate; this ray exits the system closest to the optical axis, producing the shortest OGL in the image space. Conversely, the ray entering the system nearest to the optical axis experiences the smallest deflection, and exits the system farthest from the optical axis, achieving the shortest OGL within the substrate and the longest one outside it. The achromaticity criterion is satisfied when the total OGLs of these two ray paths are equal. This occurs for two different OGL values (\(l_{\mathrm g}^{(1)} = 2581.9\,\upmu {\mathrm{m}}\) and \(l_{\mathrm g}^{(2)} = 2628.6\,\upmu {\mathrm{m}}\)) at two different wavelengths (λ(1) = 494.1 nm and λ(2) = 604.5 nm), resulting in two stationary points in the focal length dispersion (Fig. 3g); at nearby wavelengths, the criterion is also approximately satisfied, resulting in an operational bandwidth of 200 nm. A detailed discussion of the design of the bilayer apochromat is presented in Supplementary Fig. S3 and the Supplementary Information.

a Illustration of a twisted achromatic bilayer metalens. b Radial portions of the phase profiles of the two metasurfaces of the metalens shown in a. c Wavelength dependence of the focal lengths of the twisted bilayer metalens shown in a and a single-layer metalens. d Ray schematic, intensity, and field distribution on a plane between the metasurfaces of the twisted metalens (left) and the axial-plane (centre) and focal-plane (right) intensity distributions at three different wavelengths. e Ray schematic (left) and axial-plane (centre) and focal-plane (right) intensity distributions for an annular single-layer metalens. f Illustration of an apochromatic bilayer metalens and an annular single-layer metalens with the same aperture and NA, which serves as a control. g Wavelength dependence of the focal lengths of the metalenses shown in f. The apochromatic bilayer metalens satisfies the achromaticity criterion at two wavelengths, λ = 494.1 nm and λ = 604.5 nm.

Illustrated in Figure 3 is a schematic drawing of light waves reflecting and/or passing through a lens element coated with two antireflection layers. The incident wave strikes the first layer (Layer A in Figure 3) at an angle, resulting in part of the light being reflected (R(o)) and part being transmitted through the first layer. Upon encountering the second antireflection layer (Layer B), another portion of the light is reflected at the same angle and interferes with light reflected from the first layer. Some of the remaining light waves continue on to the glass surface where they are again both reflected and transmitted. Light reflected from the glass surface interferes (both constructively and destructively) with light reflected from the antireflection layers. The refractive indices of the antireflection layers vary from that of the glass and the surrounding medium (air). As the light waves pass through the antireflection layers and glass surface, a majority of the light (depending upon the incident angle--usual normal to the lens in optical microscopy) is ultimately transmitted through the glass and focused to form an image.

Ding, F. et al. A review of gap-surface plasmon metasurfaces: fundamentals and applications. Nanophotonics 7, 1129–1156 (2018).

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There is a wealth of information inscribed on the objective barrel. Briefly, each objective has inscribed on it the magnification (e.g. 10x, 20x or 40x etc.); the tube length for which the objective was designed to give its finest images (usually 160 millimeters or the Greek infinity symbol); and the thickness of cover glass protecting the specimen, which was assumed to have a constant value by the designer in correcting for spherical aberration (usually 0.17 millimeters). If the objective is designed to operate with a drop of oil between it and the specimen, the objective will be engraved OIL or OEL or HI (homogeneous immersion). In cases where these latter designations are not engraved on the objective, the objective is meant to be used dry, with air between the lowest part of the objective and the specimen. Objectives also always carry the engraving for the numerical aperture (NA) value. This may vary from 0.04 for low power objectives to 1.3 or 1.4 for high power oil-immersion apochromatic objectives. If the objective carries no designation of higher correction, one can usually assume it is an achromatic objective. More highly corrected objectives have inscriptions such as apochromat or apo, plan, FL, fluor, etc. Older objectives often have the focal length (lens-to-image distance) engraved on the barrel, which is a measure of the magnification. In modern microscopes, the objective is designed for a particular optical tube length, so including both the focal length and magnification on the barrel becomes somewhat redundant.

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The beam deflectors were fabricated using standard nanofabrication tools and techniques. We first deposited amorphous silicon on a fused silica substrate. The nano-post patterns were defined in a layer of resist using electron beam lithography and transferred to the amorphous silicon layer via a plasma etching process. The protective SU-8 layer was spun before the deposition of gold on the opposite side of the substrate. Optical and scanning electron images of the fabricated devices are shown in Fig. 4c.

Types ofmicroscopeobjectives

Kamali, S. M. et al. A review of dielectric optical metasurfaces for wavefront control. Nanophotonics 7, 1041–1068 (2018).

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Arbabi, A. et al. Miniature optical planar camera based on a wide-angle metasurface doublet corrected for monochromatic aberrations. Nat. Commun. 7, 13682 (2016).

The simplicity of the OGL design paradigm facilitates the discovery of unconventional optical components and systems. Although all metasystems discussed in this work comprise only two diffractive elements separated by a planar substrate, our design framework is applicable to systems composed of arbitrarily many refractive or diffractive elements, including refractive–diffractive hybrids. Systems of three or more metasurfaces may provide sufficiently many degrees of freedom to account for higher-order dispersion terms12 or correct off-axis aberrations26, thus enabling a new class of optical systems that can achieve any desired chromatic response.

Nagar, J., Campbell, S. D. & Werner, D. H. Apochromatic singlets enabled by metasurface-augmented GRIN lenses. Optica 5, 99–102 (2018).

Chromatic dispersion has a long history in optical science. Newton’s seminal discovery that white light is formed of constituent colours1 relied on triangular glass prisms, which, like other refractive elements, are dispersive due to their wavelength-dependent refractive indices2. In contrast, diffraction gratings and flat lenses exhibit a strong dispersion that is not material dependent but is instead considered inherent to the diffraction phenomenon3. Different chromatic dispersions are desirable in different optical systems. Wideband imaging systems used in photography, astronomy, and microscopy should be achromatic because chromatic dispersion blurs images and is regarded as an aberration. On the other hand, optical systems such as spectrometers benefit from strong chromatic dispersion. The traditional approach for engineering chromatic dispersion is based on cascading refractive elements made of different materials2 or pairing refractive and diffractive elements4.

The clearance distance between the closest surface of the cover glass and the objective front lens is termed the working distance. In situations where the specimen is designed to be imaged without a cover glass, the working distance is measured at the actual surface of the specimen. Generally, working distance decreases in a series of matched objectives as the magnification and numerical aperture increase (see Table 1). Objectives intended to view specimens with air as the imaging medium should have working distances as long as possible, provided that numerical aperture requirements are satisfied. Immersion objectives, on the other hand, should have shallower working distances in order to contain the immersion liquid between the front lens and the specimen. Many objectives designed with close working distances have a spring-loaded retraction stopper that allows the front lens assembly to be retracted by pushing it into the objective body and twisting to lock it into place. Such an accessory is convenient when the objective is rotated in the nosepiece so it will not drag immersion oil across the surface of a clean slide. Twisting the retraction stopper in the opposite direction releases the lens assembly for use. In some applications (see below), a long free working distance is indispensable, and special objectives are designed for such use despite the difficulty involved in achieving large numerical apertures and the necessary degree of optical correction.

Here, \(n_{m_{\mathrm g}} = {\mathrm d}(\omega n_m)/{\mathrm d}\omega\) represents the group index for the mth material, and \(\varphi {\prime}_m = \partial \varphi _m/\partial \omega\) is the dispersion of the phase imparted by the mth surface. The travel time for a narrowband pulse along the ray’s trajectory is the group delay \(\tau _{\mathrm g} = \Delta \Phi /\Delta \omega\); hence, we refer to \(l_{\mathrm g} = c\tau_{\mathrm g}\) as the OGL. In the following, we will describe surfaces with frequency-dependent phase profiles \(\left( {\varphi {\prime} \ne 0} \right)\) as ‘dispersive’ and those with frequency-independent profiles \(\left( {\varphi {\prime} = 0} \right)\) as ‘non-dispersive.’ Although their phase profiles do not change with frequency, non-dispersive surfaces do disperse light (consider, e.g., a diffraction grating).

Objectivelensmicroscopefunction

Chen, W. T. et al. A broadband achromatic metalens for focusing and imaging in the visible. Nat. Nanotechnol. 13, 220–226 (2018).

Arbabi, A. et al. Dielectric metasurfaces for complete control of phase and polarization with subwavelength spatial resolution and high transmission. Nat. Nanotechnol. 10, 937–943 (2015).

Newton, I. A letter of Mr. Isaac Newton, Professor of the Mathematicks in the University of Cambridge; containing his new theory about light and colors: sent by the author to the publisher from Cambridge, Febr. 6. 1671/72; in order to be communicated to the R. Society. Philos. Trans. R. Soc. 6, 3075–3087 (1671).