Scanningobjectivelens

With a 60x or 100x objective the detail that you see in the image will be very dependent on the numerical aperture (NA) of the lens, the NA of the illumination and the quality of the lens. The theoretical resolution of these lenses may be 0.4 to 0.7 µm, but that theoretical limit will only be reached with a good lens and good illumination. @r.w.bowman wrote this about resolution and magnification. A 100x lens will usually require an immersion oil between the lens and the sample. The ‘160/0.17’ on the lenses indicates that they are designed for a ‘tube length’ of 160mm, and that they are designed to be used looking through a coverslip of 0.17mm thickness.

I have found this one: AmScope 60X Achromatic Microscope Objective for Compound Microscope A60X V300|Instrument Parts & Accessories| - AliExpress

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The ‘magnification’ does not have a lot of meaning on a digital microscope - if you view it on a phone screen the image size is very different from a 70" 4K screen, but the detail in the image is the same.

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Bestobjective lenses microscope

See also this thread Comparison of a pi-camera and a 20x optical lens - Contributions - OpenFlexure Forum which has some 40x and 50x at the end.

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Objectivelens magnification

Types ofobjective lenses

This here is more expensive, however, it states it is a plan objective lens, and has a parfocal length of 45mm so looks like it is the ticket. I will purchase one of these in the next week or so. I do see the “working distance” is only 0.49mm for the 60x and 0.23mm for the 100x. That seems awfully small. Is this unusual?

I’m looking for a microscope objectives to look at microbes and such, and after doing some reading I think 60x magnification is what I want.

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Screen ghosting is a display phenomenon where actions on the screen leave faint afterimages or traces of previous visuals

Ocular lensmicroscope

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Screen ghosting is rare, and most Mac users will never experience it. However, if you do experience it, you will no doubt be frustrated by the way it detracts from your enjoyment of playing games or watching videos. The good news is that, like most problems you will run into when using your Mac, it can usually be fixed. Follow the steps above to find out the cause of screen ghosting on your Mac and to fix it.

Screen ghosting is the name given to the phenomenon where action on the screen leaves behind a trace of what was on screen a split second earlier. So, for example, in a driving game, as you move around a track, you may see faint images left behind from what was on screen a frame or two earlier. The ghost images fade quickly but it can be very distracting and detract from the enjoyment of a game. So, if you notice it, you should try and fix it as soon as possible.

The lens is likely to fit and work, I am not sure of the image quality. There is one part of the specification that is not in that screenshot and is often not stated. The parfocal distance is usually 45 mm. The Openflexure microscope V6.1.5 is designed to fit 45mm parfocal distance with the slide riser fitted, or should work with the next most common 35mm parfocal distance without the slide riser. The V7.0.0-Alpha2 is designed to work with 45mm only. The specification does not include the word ‘Plan’, so I would assume that it is indeed achromatic, but not plan. This means that a flat slide will not be in focus all across the slide. How much this matters depends on what you are wanting to look at. If you are planning to scan and tile images then you really do need a Plan corrected objective.

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As with any problem where you don’t know the cause, the first step in solving the screen ghosting issue is to identify why it’s happening. Doing that is a process of trial and error, eliminating possibilities one by one.

The working distance will be very small, usually much less than 1mm for 60 or 100x . The sample should be under a coverslip on the same side of the slide as the objective lens, so the slide ‘upsidedown’ on an inverted microscope design like this. It is possible to get objective lenses with long working distances, but they get expensive very quickly. They are often designated ‘metallurgical’ instead of ‘biological’ lenses, and are not designed for use with any coverslip or glass between the sample and the lens - they are for looking at metal and mineral surfaces. There is one I found that is not too expensive, and also is designed specifically to look through a microscope slide from underneath, so with 1mm of glass, and with a working distance of a few mm. Motic Microscope LWD 40x Plan Objective Lens (motic-microscope.com). It is 40x so has a larger field of view than 60x, but at 0.5NA the theoretical resolution is still about 1µm. I have a couple of Motic microscopes and the lenses are pretty good, I have not yet tried this particular lens.

Will this do what I want? It appears to be achromatic, (ie plan corrected as I understand it?) and is designed for a 160mm objective tube? The thread appears to be DIN, which I understand, fits RMS? Thoughts?

There are lots of different problems you are likely to come across when you use your Mac. And the more you demand from it, the more likely you are to face issues. Most can be easily fixed, others are more difficult. One issue that may crop up, especially if you use an external display with your Mac, is screen ghosting. This typically happens when you’re playing fast-paced games, but can also occur when you’re watching videos or animation. In this article, we’ll explain what screen ghosting is, why it happens and how to fix it.

There are a number of reasons why you may be experiencing screen ghosting. But ultimately, it’s caused by pixels in the display not responding quickly and accurately to the data they are being sent. For example, on an LCD screen, which has three elements in an array for every pixel – one to display blue light, one red, and one green – it could be that the liquid crystal elements themselves are not brightening or darkening quickly enough to respond to high frame rate action. This is more likely when your display resolution is set to a high refresh rate. On an LED display, the diodes may be having a similar problem. That could be caused by the age of the display in terms of hours used. LEDs and LCD arrays have a limited lifespan. Once they reach the end of that lifespan, they often tend to develop faults. Individual pixels may fail to light up – a phenomenon known as dead pixels – and ghosting might occur. However, screen ghosting could also be caused by the connection between the display and your Mac or by a GPU fault, a software issue, or even interference from radio waves from a WiFi router.