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The MMSphere 2.0 is the best at-home LED green light therapy device, endorsed by dermatologists for its safety and effectiveness. This device can work ...

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Keywords: cat; feline; feline chronic enteropathy; fluorescence imitating brightfield imaging; inflammatory bowel disease; low-grade intestinal T-cell lymphoma; lymphocytic-plasmacytic enteritis.

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Fluorescence imitating brightfield imaging (FIBI) is a novel microscopy method that allows for real-time, nondestructive, slide-free tissue imaging of fresh, formalin-fixed, or paraffin-embedded tissue. The nondestructive nature of the technology permits tissue preservation for downstream analyses. The objective of this observational study was to assess the utility of FIBI compared with conventional hematoxylin and eosin (H&E)-stained histology slides in feline gastrointestinal histopathology. Formalin-fixed paraffin-embedded full-thickness small intestinal tissue specimens from 50 cases of feline chronic enteropathy were evaluated. The ability of FIBI to evaluate predetermined morphological features (epithelium, villi, crypts, lacteals, fibrosis, submucosa, and muscularis propria) and inflammatory cells was assessed on a 3-point scale (0 = FIBI cannot identify the feature; 1 = FIBI can identify the feature; 2 = FIBI can identify the feature with more certainty than H&E). H&E and FIBI images were also scored according to World Small Animal Veterinary Association (WSAVA) Gastrointestinal Standardization Group guidelines. FIBI identified morphological features with similar or, in some cases, higher confidence compared with H&E images. The identification of inflammatory cells was less consistent. FIBI and H&E images showed an overall poor agreement with regard to the assigned WSAVA scores. While FIBI showed an equal or better ability to identify morphological features in intestinal biopsies, its ability to identify inflammatory cells is currently inferior compared with H&E-based imaging. Future studies on the utility of FIBI as a diagnostic tool for noninflammatory histopathologic lesions are warranted.

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The author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: Dr Richard M. Levenson and Dr Farzad Fereidouni are co-founders of Histolix Inc.; the remaining authors have stated that they have no conflict of interest.

You already know how to convert 66.6 millimeters to centimeters; isn't it? Using its symbol, 66.6 millimeters is written as 66.6 mm, and 6.66 centimeters are ...