Koehler illumination is a microscope technique that provides superior control over the light rays during brightfield microscopy by aligning and focusing the microscope, ensuring the best resolution and contrast, as well as a bright, evenly illuminated background for your images. Koehler illumination is critical for the following advanced contrasting techniques: Phase contrast, Varel contrast, Hoffman Modulation, PlasDIC and DIC. Prior to setting up and aligning the advanced contrasting components, the microscope’s Koehler illumination must be aligned.

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The last step is to adjust the condenser aperture iris to match the NA (numerical aperture) indicated on the objective. The aperture iris can also be stopped down to 60% of the NA setting (trading image resolution for contrast.) An alternative way to set the condenser aperture is to remove the eyepiece and look down the eye tube at the back of the objective and adjust the aperture diaphragm until approximately 90% of the area is illuminated (a setting that visually looks very near the edge of the objective but is not quite fully to the edge).

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Place a sample slide on the stage and bring it into focus with the selected target objective (it may be helpful to start by focusing with a lower magnification objective and then stepping up to the target objective). You may have to adjust the opening of the field diaphragm to get an edge into the field of view (this condenser focus point tends to be near the specimen end of the condenser focus travel).

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Infrared filters, infrared covers and covert infrared lens covers. Infrared filter covers (blackout covers) are used to block the emission of visible light.

Adjust the diopters on the microscope eyepieces to zero. Adjust the interpupillary distance of both eyepieces to fit your eyes.

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In order to set up a microscope for Koehler illumination, the microscope has to have a vertically adjustable, centerable condenser and field diaphragm as illustrated in the figure below.

Caltrans is activating phase two of its adaptive metering lights along about 20 miles of the freeway between Candlestick Point in San Francisco and Whipple Avenue in Redwood City.

ADOT continues to upgrade the Phoenix area's network of adaptive freeway ramp meters used to improve the flow of traffic during busy rush hours.

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Gradually open the field iris and make final tweaks of the centration with the field iris at the very edge of the field of view. Then open the field iris just outside the field of view.

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"For example, if there’s a crash that happens down the highway, this responsive system will be able to slow down the meters, slow down traffic from entering the freeway and reduce congestion," Caltrans spokesperson Jeneane Crawford says.

Determine which objective will be used most for your detailed work. It is recommended that you start with a 10X. This will be the target objective for aligning the Koehler illumination. For publication images, the Koehler illumination should be set for each objective used to photograph specimens.

The lights operate between 5 a.m. and 8 p.m. seven days a week. Caltrans says the final two phases of the rollout will be finished by October.

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While the appearance of the metering lights hasn't changed, the devices are now connected to software that responds to traffic conditions in real time. The agency says the system is a proven tool for congestion management and enhances traffic safety in the process.