What is Unpolarized Light? - polarized and unpolarized light
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The polarization of light incident upon a grating is defined by the orientation of its electric and magnetic fields relative to the plane of incidence, where the plane of incidence is defined by the two vectors being the propagation vector of the illumination and the grating normal.
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Medium-wave or Ultraviolet B (UVB) lamps emit light in wavelengths from 280 to 315nm and are used for gel electrophoresis, viewing, and other procedures. UVB light is also used in transilluminators. Gels are stained with fluorescent dyes that bind to the nucleic acids to make them visible under UVB light. This technique helps researchers size PCR products, quantify DNA, and verify the integrity of extracted RNA.
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Longwave or Ultraviolet A (UVA) lamps emit UV light from 315 to 400nm. UVA lamps are used for bacterial identification, staining, electrophoresis, pesticide analysis, fluorescence photography, visualizing nucleic acids, and other genetic work. UVA is also useful for monitoring chlorination, in fluorochemistry, titrations, thin-layer chromatography (TLC), food contamination identification, water testing, and forensic applications.
TE (Transverse Electric) polarized light is characterized by its electric field being perpendicular to the plane of incidence. For TE light, the magnetic field – always perpendicular to the electric field in isotropic materials – thus lies in the plane of incidence.
TM (Transverse Magnetic) polarized light is characterized by its magnetic field being perpendicular to the plane of incidence. For TM light, the electric field – always perpendicular to the magnetic field in isotropic materials – thus lies in the plane of incidence.
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Circular polarization occurs when the two orthogonal electric field component vectors are of equal magnitude and are out of phase by exactly 90°, or one-quarter ...
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For the purpose of defining the diffraction efficiency of a grating, unpolarized light is equivalent to the average of TE and TM efficiency.
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Shortwave or Ultraviolet C (UVC) lamps emit light in the 100 to 280nm range. Often used as germicidal lamps, UVC is commonly used to disinfect surfaces in laboratories and food-processing facilities and to disinfect water. UVC lamps are also used to cure polymers and for fluorescent analysis, in fluorochemistry, pesticide analysis, DNA and other biochemical testing, microbiological testing, electrophoresis, and photochemistry.
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