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if you shine a beam of polarised monochromatic light (light of only a single frequency - in other words a single colour) through a solution of an optically ...
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In optics, the distance from the middle of the lens to its focal point. Light rays run in parallel to one another, while lenses distort light rays.
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Many high quality advanced microscopes that are made with Koehler illumination, if not set up properly, will fail to yield the highest quality images the microscope is capable of. Follow the steps below in order to set up Koehler illumination properly. 1. Place a high contrast slide on the stage, turn on the light and move the 4x or 10x objective lens into position. Set the rheostat control for the light so it is at a comfortable illumination and not overly bright for your eyes. Adjust the condenser height (adjustment knob shown at left) so the top of the condenser is about 0.5cm from the bottom of the microscope slide. 2. Adjust the condenser iris diaphragm (located on the front of the condenser) so it is closed down almost all the way. This will create a dark circle around your image when you look through the eyepieces. Make sure this dark image is centered in the field of view. If the dark circle is off to one side, use the condenser centering screws (Figure 5b below) to center the condenser so the image is directly in the middle of your field of view.
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by TW Cronin · 2011 · Cited by 147 — We review the current state of knowledge concerning how polarization and polarization patterns are formed in nature, emphasizing linearly polarized light.
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The UVA intensity is 2.5 mW/cm^2 max and the lamp requires 4 AA batteries (not included). The figure below shows a plot of the relative UV irradiance emitted by ...
Koehler illumination is obtained by focusing and centering microscopy light on the field of view in order to increase contrast and resolution. Koehler illumination was first introduced in 1893 by August Köhler of the Carl Zeiss corporation as a method of providing optimum specimen illumination. Microscope manufacturers have since designed microscopes with Koehler illumination so the collector lens and other optical components will project an enlarged and focused image of the lamp filament onto the plane of the aperture diaphragm of a properly positioned substage condenser, resulting in higher contrast and resolution images.
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You can definitely tell it has always had the caps and skylight filter on it because the glass is perfect with no scratches, haze, or internal dust. I have ...