Illumina | Sequencing and array solutions to fuel genomic ... - illumin logo
These cube beam splitters have no beam shift and can be easily integrated with 0-degree angle of incidence. The reflected and transmitted optical path lengths are equal, and compared to other options, they allow you to shorten the optical path of a system. Their only disadvantages are the heavy construction—each piece is solid glass—and the fact that they are both difficult and expensive to manufacture in large sizes. We recommend these beam splitters in situations where simplified mounting and durability are critical.
The working distance of the objective lens refers to the distance between the sample surface and the front end of the objective lens after the microscope is accurately focused.
Please contact us to discuss beam splitter designs or custom orders. Our team is experienced in optical design and can help you determine the ideal beam splitter for your situation. We can assist you from blueprint to prototype to full-scale production of your optical product. Custom beam splitters tailored to your specific wavelength ranges and tolerance levels are also available upon request.
The cube should always be oriented so that incident light enters the coated prism to minimize energy passing through the optical cement. Entering from the wrong side will cause more than triple the amount of energy to pass through the delicate cement layer, which may degrade over time if exposed to high-power light sources. We place a reference mark on the ground side of the coated prism to ensure proper orientation.
The field of view refers to the size of the surface area of the sample observed in the microscope. The field of view is inversely proportional to the magnification of the objective lens. Generally, the diameter of the first magnified real image of an ordinary objective lens is 18mm, and the diameters of the field of view of an objective lens with magnification of 10x, 40x and 100x are 1.8 mm, 0.45mm and 0.18mm respectively. The diameter of the first enlarged real image of the head-up field objective lens can reach 28mm, and the market scope is greatly increased.
Alpha Industrial Park, Tu Thon Village, Ly Thuong Kiet Commune, Yen My District, Hung Yen Province Vietnam 17721 +84 221-730-8668 rfqvn@shanghai-optics.com
Cubic beam splitters usually consist of two right-angle prisms carefully cemented together with optical glue. The thickness of the optical glue depends on the wavelength of light for the intended application. One inner surface of one of the prisms is coated with a partially reflective metal di-electric coating.
diaphragmThere are two diaphragms in the illumination system of metallographic microscope, namely aperture diaphragm and field diaphragm.
As the name suggests, these optics divide a light beam into two separate beams, splitting light according to its polarity. They are often used to transmit p-polarized light while reflecting all s-polarized light in a different direction. The polarizing beam splitters we manufacture at our factory include cubic beamsplitters, plate beamsplitters, and lateral displacement beamsplitters.
The above two kinds of objective lenses are classified according to the correction degree of spherical aberration and chromatic aberration. The horizontal field objective lens is based on the breadth of field of view plane correction. The head-up field objective lens can correct the curvature of the image field well. The flat-field objective lens can be divided into flat-field achromatic objective lens and apochromatic objective lens, and the correction of spherical aberration and chromatic aberration is the same as that of achromatic objective lens and apochromatic objective lens respectively. The characteristic of this kind of objective lens is that it significantly expands the flat range of the image field, making the whole field of view clearer, suitable for observation and more conducive to photography.
Room 609, 6/F, Global Gateway Tower, No.63 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong +852-54993705 info@shanghai-optics.com
Laser beam splitters separate a laser beam into two sections and are typically designed to reflect part of the beam, either differentiated by wavelength or polarization. For laser applications, cubic, plate, or lateral displacement beam splitters are all possible options, and each can be custom-made for laser wavelengths. Surface quality is essential for any laser optics, and our state-of-the-art machinery and strict quality control ensure that every optic leaving our factory meets all applicable standards.
The higher the magnification of the objective lens, the shorter the working distance! Therefore, it is necessary to be extra careful when observing the focusing, and generally, the objective lens should run in the direction away from the object.
Laser beam combiners are optical devices designed to merge two or more laser beams into a single, unified beam. This is achieved by selectively combining beams based on their wavelength or polarization. These devices are commonly used in applications requiring multiple laser sources to be integrated into a single output, such as in scientific research, telecommunications, and high-precision manufacturing. By effectively merging laser beams, these combiners help optimize space and enhance system performance.
According to the different degrees of aberration correction, objective lenses are generally divided into achromatic objective lenses, apochromatic objective lenses and flat-field objective lenses.
Field of view diaphragmGenerally, a microscope has a field of view besides the aperture stop. Relative to the light source, its position is behind the aperture stop. Adjusting the field diaphragm can change the size of the microscope field of view without affecting the resolution of the objective lens. Proper adjustment of the field diaphragm can also reduce the reflection and glare in the lens barrel and improve the contrast and quality of imaging. However, it should be noted that if the field diaphragm is too small, the observation range will be too narrow, and it should generally be adjusted to the same size as the eyepiece field.
Aperture diaphragmAperture diaphragm is used to control the thickness of incident light beam, and its position is close to the light source. Generally, the aperture diaphragm of a microscope can be continuously adjusted. When the aperture diaphragm is reduced, the beam entering the objective lens becomes thinner, and the light does not pass through the edge of the objective lens group, so the spherical aberration is greatly reduced. However, the beam thinning will reduce the aperture angle of Wu Jingdi, which will reduce the actual numerical aperture and resolution. When the aperture diaphragm is enlarged, the incident beam becomes thicker and the aperture angle of the objective lens increases, which can make the light fill the rear lens of the objective lens. At this time, the numerical aperture can reach the rated value and the resolution is improved. However, due to the increase of spherical aberration and the increase of internal reflection and glare in the lens barrel, the imaging quality will be reduced. Therefore, the aperture diaphragm has a great influence on the imaging quality, and it must be properly adjusted when it is used. It should not be too large or too small, and its appropriate degree should be based on the lens after the light beam fills the objective lens, and judged according to the clarity of the imaging. After replacing the objective lens, the aperture stop must be adjusted properly. But it is not used to adjust the brightness of the field of view.
Our dichroic beamsplitters feature very steep edges, and our narrow spectral band edge tolerances ensure maximum spectral stability.
Shanghai Optics manufactures a wide range of high-quality beamsplitters optimized for different applications. Our selection includes plate and cube designs, offering polarizing, non-polarizing, and dichroic options. All our custom beam splitters are made from premium glass, ensuring superior surface quality and tight tolerance on all optical parameters.
If an incident beam needs to be divided into two displaced parallel beams, a lateral displacement beam splitter is ideal. Our precision lateral displacement beam splitters, consisting of a rhomboid prism cemented to a right-angle prism, ensure that the exiting beams have no more than 30 arcseconds of deviation from parallel. A multi-layer anti-reflection coating on both entrance and exit faces provides increased efficiency.
A beam splitter (or beamsplitter) is an optical component used to split incident light into two separate beams, typically based on wavelength or polarity. This precise ability to split light by wavelength makes beam splitters essential in various fields, including laser systems, semiconductor technologies, and photonics instrumentation. Additionally, beam splitters can function in reverse to combine two beams into one.
achromatic objectiveThe correction of spherical aberration by achromatic objective lens is limited to yellow and green light, and only red and green light are corrected for chromatic aberration. Therefore, the achromatic objective still has residual chromatic aberration, and the image domain curvature still exists. When using achromatic objective lens, yellow-green light or yellow-green color filter can reduce aberration.
A dichroic beam splitter, or dichroic mirror, is an optical filter that transmits selected wavelengths while reflecting others. These beam splitters are typically used at non-normal angles of incidence. If placed at a 45-degree orientation to the incident light, the reflected light will be at a 90-degree angle. When selecting the ideal dichroic beam splitter for your application, consider the following:
Pellicle beam splitters are ultra-thin optical components designed to split incident light into two separate beams without significant beam displacement or optical path length changes. Made from a thin membrane stretched over a frame, pellicle beam splitters are ideal for high-precision applications where minimal optical distortion and interference are critical. Their thin profile reduces the potential for ghost reflections and beam shifts, making them well-suited for applications like interferometry, microscopy, and high-speed imaging. However, due to their delicate structure, they are typically used in low-power laser systems to avoid damage. Pellicle beam splitters are valued for their ability to split light while maintaining alignment accuracy and image quality.
A non-polarizing beam splitter is used to split light independently of the polarization state. These filters have very small polarity dependences (typically about 3-6%). Our non-polarizing beam splitters are used in laser beam manipulation and interferometry, and we offer both plate and cubic options. These dichroic mirrors can be customized with a metallic coating for partial reflection for the wavelength of your chosen wavelength.
Plate beam splitters, on the other hand, are lighter, less expensive, and can be easily manufactured in any size. They consist of a flat, thin glass plate with a coating on the first surface of the substrate. This coating splits the incident beam by a specified ratio. The reflected and transmitted optical paths have different lengths, and there is a beam shift in transmitted light. Although these optics are often designed for a-45 degree angle of incidence, setup may require extra alignment time. Plate beam splitters are cheaper to manufacture than cube beam splitters.
Apochromatic objective lensApochromatic objective lens is a high quality objective lens, which can correct the chromatic aberration in three wave regions (actually equal to the whole visible light range). Spherical aberration correction can reach the range of green and purple light, but it has no fundamental improvement on image domain bending. This kind of objective lens has no restrictions on the light source, and generally has a large numerical aperture and high imaging quality, which is suitable for high-magnification observation.