The Iris family of sCMOS cameras deliver up to a 15 megapixel sensor with a 25 millimetre field of view for high-resolution imaging over a large imaging area.

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Biochip, genomics and microarray detection represent a large mix of applications with varying needs of a scientific camera.

The Evolve family of cameras are high-resolution, back-illuminated EMCCD providing high sensitivity for the lowest light applications.

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All cameras are controllable with the PVCAM driver and supported in Ocular software. The PVCAM driver SDK can also be used integrate into other software packages.

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The QImaging CCD family of scientific cameras are designed with solutions for electrophysiology, long stare, color imaging, documentation and live cell imaging.

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Cooled, low-noise CMOS cameras designed for integration. With unprecedented thermal control, Retiga E cameras are capable of exposures over an hour!

High content imaging is primarily concerned with the automated analysis of large cell populations where the goal is to process as many cells as possible in the fastest time with the highest resolution.

Physics and biophysics imaging encompasses a wide range of techniques used to interrogate physical phenomena using high tech imaging systems.

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The Iris family of sCMOS cameras deliver up to a 15 megapixel sensor with a 25 millimetre field of view for high-resolution imaging over a large imaging area.

A sensor for a scientific camera needs to be able to detect and count photons, and then convert them into electrical signals. This involves multiple steps, the first of which involves detecting photons. Scientific cameras use photodetectors, where photons that hit the photodetector are converted into an equivalent amount of electrons. These photodetectors are typically made of a very thin layer of silicon. When photons from a light source hit this layer, they are converted into electrons. A layout of such a sensor can be seen in Figure 2.

CMOS made scientific. The Moment is a true global shutter CMOS camera with an ultra-compact form factor, powered through USB 3.2 Gen 2.

Camera sensors are at the heart of the camera and have been subject to numerous different iterations over the years. Researchers are constantly on the lookout for better sensors which can improve their images, bringing better resolution, sensitivity, field of view and speed. The three main camera sensor technologies are charge-coupled device (CCD), electron-multiplied CCD (EMCCD) and complementary metal-oxide-semiconductor (CMOS), all of which are discussed in separate documents.

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Making pixels smaller allows for more to fit on a sensor, but if pixels become too small they won’t be able to detect as many photons, which introduces the concept of compromise in camera design between resolution and sensitivity.

Cooled, low-noise CMOS cameras designed for integration. With unprecedented thermal control, Retiga E cameras are capable of exposures over an hour!

Video camera

Supplying custom cameras to instrument designers for most of our 40 year history, we will work with you every step of the way.

In microscopy, it is vital to have some form of contrast or stain that gives areas of the sample color and makes it possible to image. Advanced fluorescence microscopy techniques take advantage of this.

High content imaging is primarily concerned with the automated analysis of large cell populations where the goal is to process as many cells as possible in the fastest time with the highest resolution.

The brand new Kinetix family of back-illuminated sCMOS cameras delivers a framerate and field of view unmatched by any other sCMOS camera.

2023110 — A polarizing beam splitter (PBS) and PBS interferometer (PBSI) can be used to illustrate the superposition principle. In this analysis the ...

Photons are particles that make up all types of electromagnetic radiation, including light and radio waves, as seen in Figure 1. The most important part of this spectrum for imaging is visible light, which ranges from 380-750 nanometers, as seen in the insert of Figure 1.

Supplying custom cameras to instrument designers for most of our 40 year history, we will work with you every step of the way.

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In addition, if sensors are too big or contain too many pixels it would massively increase the computational power needed to process the output information, which would slow down the image acquisition. Large information storage would be needed, and with researchers taking thousands of images in experiments lasting months/years, having a bloated sensor would quickly become an issue as the storage fills up. Due to these reasons the overall sensor size, pixel size and the number of pixels is carefully optimized in camera design.

The Evolve family of cameras are high-resolution, back-illuminated EMCCD providing high sensitivity for the lowest light applications.

The most important aspect of a scientific camera is the ability to be quantitative, being a camera that can measure specific quantities of something. In this case, the camera is measuring light, and the most basic measurable unit of light is the photon.

In order to fit more pixels onto sensors, pixels have become very small, but as there are millions of pixels the sensors are still quite large in comparison. The Prime BSI camera has 6.5 µm square pixels (an area of 42.25 µm2) arranged in an array of 2048 x 2048 pixels (4.2 million pixels), resulting in a sensor size of 13.3 x 13.3 mm (area of 177 mm2 or 1.77 cm2) and a diagonal of 18.8 mm.

See what others are doing. Stories and images from scientists using our high-performance sCMOS, EMCCD and CCD cameras to advance their research.

201921 — The Collimator Fixture is configurable to your working distances and fields of view with an adjustable lens-to-chart distance, interchangeable collimating ...

The Prime series of 95% quantum efficient, back-illuminated sCMOS cameras are designed to support the most demanding, low-light research applications

Biochip, genomics and microarray detection represent a large mix of applications with varying needs of a scientific camera.

CMOS made scientific. The Moment is a true global shutter CMOS camera with an ultra-compact form factor, powered through USB 3.2 Gen 2.

2007831 — Physical optics is the study of the wave properties of light, which may be roughly grouped into three categories: interference, diffraction, and ...

When exposed to light, each pixel of the sensor detects how many photons come into contact with it. This gives a map of values, where each pixel has detected a certain number of photons. This array of measurements is known as a bitmap and is the basis of all scientific images taken with cameras, as seen in Figure 4. The bitmap of an image is accompanied by the metadata, which contains all the other information about the image, such as the time it was taken, camera settings, imaging software settings, and microscope hardware information.

All cameras are controllable with the PVCAM driver and supported in Ocular software. The PVCAM driver SDK can also be used integrate into other software packages.

How camera Works

As microscopes typically use visible light in the form of a lamp or laser, a scientific camera is essentially a device that needs to detect and count photons from the visible light portion of the spectrum (380‑750 nm), but some applications can also benefit from detection in the UV and IR regions. To do this, scientific cameras use sensors.

However, having just one block of silicon means it isn’t clear where the photons come from when they land, it would only be possible to know that they landed. By making a grid of many tiny squares of silicon, photons can be both detected and localized. These tiny squares are referred to as pixels, and technology has developed to the point where you can fit millions of them onto a sensor. When a camera advertises as having 1 megapixel, this means the sensor is an array of one million pixels. This concept is explored in Figure 3.

What is the working distance of the Microscope lenses 1x, 2.5x,4x for the SC systems? Here are some optical data.

The QImaging CCD family of scientific cameras are designed with solutions for electrophysiology, long stare, color imaging, documentation and live cell imaging.

In microscopy, it is vital to have some form of contrast or stain that gives areas of the sample color and makes it possible to image. Advanced fluorescence microscopy techniques take advantage of this.

Refractive index is always greater than one because light travels fastest in a vacuum. For glass, the range of refractive indices is usually between 1.4 and 1.7 ...

SLR camera

The brand new Kinetix family of back-illuminated sCMOS cameras delivers a framerate and field of view unmatched by any other sCMOS camera.

See what others are doing. Stories and images from scientists using our high-performance sCMOS, EMCCD and CCD cameras to advance their research.

A scientific camera is a vital component of any imaging system. These cameras are designed to quantitatively measure how many photons of light hit which part of the camera sensor. Photons generate electrons (photoelectrons), which are stored in sensor pixels and converted to a digital signal, which is displayed as an image. This process is optimized at every stage in order to produce the best possible image depending on the signal received. Read on for more about the three main types of sensor, the CCD, EMCCD and CMOS.

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These grey levels vary depending on the number of electrons stored in the sensor pixels. It is also related to two important imaging concepts, offset and gain. Offset is the grey level value from no electrons, the base level. Gain is the conversion factor of electrons to grey levels, for example, 60 electrons can be converted to 60 grey levels (1x gain), or 140 grey levels (2.3x gain). Further relationships between grey levels and the image that appears on the monitor are due to the imaging software display settings. These display settings affect what the grey levels actually look like, as they are otherwise arbitrary (what does 140 grey levels look like, is it the whitest or blackest pixel?) and reliant on the dynamic range of other values across the sensor. These main stages of imaging with a scientific camera are consistent across all modern camera technologies, but there are several different archetypes.

Physics and biophysics imaging encompasses a wide range of techniques used to interrogate physical phenomena using high tech imaging systems.

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Cameras are incredible tools that allow us to capture and make sense of the visible world around us. Most mobile phones made today come with a camera, meaning that more people than ever are becoming familiar with camera software and taking images. But one of the largest applications of cameras is for scientific imaging, in order to take images for scientific research. For these applications, we need carefully manufactured scientific cameras.

The Prime series of 95% quantum efficient, back-illuminated sCMOS cameras are designed to support the most demanding, low-light research applications