Keep work areas clean and dry. Cluttered work areas and benches invite accidents and injuries. Good housekeeping and a well-planned layout of temporary wiring will reduce the dangers of fire, shock, and tripping hazards.

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A gas that is flammable in a mixture of 13 percent or less (by volume) with air, or the flammable range with air is wider than 12 percent regardless of the lower limit, at atmospheric temperature and pressure.

Entrances to rooms and other guarded locations containing exposed live parts must be marked with conspicuous warning signs forbidding unqualified persons to enter. Live parts of electric equipment operating at 50 volts or more must be guarded against accidental contact. Guarding of live parts may be accomplished by:

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All electrical devices fabricated for experimental purposes must meet state and University construction and grounding requirements. Extension cords, power strips, and other purchased electrical equipment must be Underwriters Laboratories (UL) listed.

To protect against electrical hazards and to respond to electrical emergencies it is important to identify the electrical panels that serve each room. Access to these panels must be unobstructed; a minimum of 3’ of clearance is required in front of every electrical panel. Each panel must have all the circuit breakers labeled as to what they control. Contact your Building Coordinator for assistance.

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As this table illustrates, a difference of less than 100 milliamperes exists between a current that is barely perceptible and one that can kill. Muscular contraction caused by stimulation may not allow the victim to free himself/herself from the circuit, and the increased duration of exposure increases the dangers to the shock victim. For example, a current of 100 milliamperes for 3 seconds is equivalent to a current of 900 milliamperes applied for 0.03 seconds in causing fibrillation. The so-called low voltages can be extremely dangerous because, all other factors being equal, the degree of injury is proportional to the length of time the body is in the circuit. Simply put, low voltage does not mean low hazard.

Electrophoresis is a commonly used laboratory technique that uses electrical energy to separate molecules such as proteins or nucleic acids by their size, structure, and electrical charge. Electrophoresis units present several possible hazards including electrical, chemical, and radiological hazards. Each of these hazards need to be addressed before using the units.

Fuses, circuit breakers, and Ground-Fault Circuit Interrupters are three well-known examples of circuit protection devices

Do not work on energized circuits. The accidental or unexpected starting of electrical equipment can cause severe injury or death. Before any inspections or repairs are made, the current must be turned off at the switch box and the switch padlocked or tagged out in the off position. At the same time, the switch or controls of the machine or the other equipment being locked out of service should be securely tagged to show which equipment or circuits are being worked on. Test the equipment to make sure there is no residual energy before attempting to work on the circuit. Employees must follow lock-out/tag-out procedures.

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Ultraviolet (UV) light boxes and handheld lamps are often used in visualizing ethidium bromide gels and pose potential exposures to UV radiation.

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The severity of the shock received when a person becomes a part of an electric circuit is affected by three primary factors:

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When performing laboratory inspections, it is a good idea to verify the location of the power panel and to open the door to ensure any breakers that are missing have breaker caps in its place. If no breaker is present and no breaker cap is covering the hole, contact your Building Coordinator for assistance.

Do not attempt electrical repairs unless you are a qualified electrical technician assigned to perform electrical work by your supervisor. Qualified individuals must receive training in safety related work practices and procedures, be able to recognize specific hazards associated with electrical energy, and be trained to understand the relationship between electrical hazards and possible injury. Fixed wiring may only be repaired or modified by Facilities Services.

Fuses and circuit breakers are devices that are placed in circuits to automatically break the circuit when the amount of the current flow becomes excessive and therefore unsafe. Fuses are designed to melt when too much current flows through them. Circuit breakers, on the other hand, are designed to open the circuit by electro-mechanical means.

In the event of an accident involving electricity, if the individual is down or unconscious, or not breathing: CALL 911 immediately. If an individual must be physically removed from an electrical source, it is always best to eliminate the power source first (i.e., switch off the circuit breaker). However, if time or circumstance do not allow this option, be sure to use a nonconductive item such as a dry board. Failure to think and react properly could make you an additional victim. If the individual is not breathing and you have been trained in CPR, have someone call 911 and begin CPR IMMEDIATELY!

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Fuses and circuit breakers are intended primarily for the protection of conductors and equipment. They prevent overheating of wires and components that might otherwise create hazards for operators.

Always review the Material Safety Data Sheet prior to working with any hazardous material. See the Chemical Hygiene Plan for more information on working with hazardous chemicals.

Laboratory personnel may be exposed to thermal hazards when heating agarose solutions. Exercise caution when using a microwave to melt agarose solutions – don’t use sealed containers, and beware of superheated liquids that may suddenly and unexpectedly boil. Let hot agarose solutions cool to 50°-60°C before adding ethidium bromide or pouring into trays. Wear insulated gloves and point the flask opening away from you.

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Typical electrophoresis units operating at 100 volts can provide a lethal shock of 25 milliamps. Take the following precautions when working with electrophoresis equipment:

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Avoid operating or working with electrical equipment in a wet or damp environment. If you must work in a wet or damp environment, be sure your outlets or circuit breakers are Ground Fault Circuit Interrupter (GFCI) protected. Temporary GFCI plug adapters can also be used, but are not a substitute for GFCI outlets or circuit breakers.

Common signs of an electrical problem include: flickering lights, warm switches or receptacles, burning odors, sparking sounds when cords are moved, loose connections, frayed, cracked, or broken wires. If you notice any of these problems, have a qualified electrician address the issue immediately.

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Electricity travels in closed circuits. Shock occurs when the body becomes a part of an electric circuit. Electric shock can cause direct injuries such as electrical burns, arc burns, and thermal contact burns. It can also cause injuries of an indirect or secondary nature in which involuntary muscle reaction from the electric shock can cause bruises, bone fractures, and even death resulting from collisions or falls. Shock occurs when person in contact with ground comes in contact with any of the following:

Many common electrical hazards can be easily identified before a serious problem exists. Read and follow all equipment operating instructions for proper use. Ask yourself, "Do I have the skills, knowledge, tools, and experience to do this work safely?"

The Ground Fault Circuit Interrupter (GFCI) is designed to shut off electric power within as little as 1/40 of a second, thereby protecting the person, not just the equipment. It works by comparing the amount of current going to an electric device against the amount of current returning from the device along the circuit conductors. A fixed or portable GFCI should be used in high-risk areas such as wet locations and construction sites.

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Use insulated tools and testing equipment to work on electrical equipment. Use power tools that are double-insulated or that have Ground Fault Circuit Interrupters protecting the circuit. Do not use aluminum ladders while working with electricity; choose either wood or fiberglass.

Determine appropriate personal protective equipment (PPE) based on potential hazards present. Before use, inspect safety glasses and gloves for signs of wear and tear, and other damage.

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Other factors that may affect the severity of shock are the frequency of the current, the phase of the heart cycle when shock occurs, and the general health of the person prior to shock. The effects of an electrical shock can range from a barely perceptible tingle to immediate cardiac arrest. Although there are no absolute limits or even known values that show the exact injury from any given amperage, the table below shows the general relationship between the degree of injury and the amount of amperage for a 60-cycle hand-to-foot path of one second's duration of shock.

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