High powerobjectivelens

Computer screens give off blue light which causes eye strain and can be damaging to your eyes in the long term.  Blue light blocking lenses do the job to shield your eyes from damaging blue light rays but reflect grey or blue on video.  In short, they may be good for your eyes but not good for your appearance on Zoom.

Some eyeglass manufacturers tout new non-glare technology that helps protect your eyes and keeps lenses glare-free on video calls. Anti-reflective (AR) coatings can be applied when you buy glasses or to ones you already own.  But note that they are still fairly new when it comes to video conferencing.  I plan to check them out and will write an update to this article.

In modern microscopes, neither the eyepiece nor the microscope objective is a simple lens. Instead, a combination of carefully chosen optical components work together to create a high quality magnified image. A basic compound microscope can magnify up to about 1000x. If you need higher magnification, you may wish to use an electron microscope, which can magnify up to a million times.

There are some important specifications and terminology you’ll want to be aware of when designing a microscope or ordering microscope objectives. Here is a list of key terminology.

Microscope objective lenses are typically the most complex part of a microscope.  Most microscopes will have three or four objectives lenses, mounted on a turntable for ease of use. A scanning objective lens will provide 4x magnification,  a low power magnification lens will provide magnification of 10x, and a high power objective offers 40x magnification. For high magnification, you will need to use oil immersion objectives. These can provide up to 50x, 60x, or 100x magnification and increase the resolving power of the microscope, but they cannot be used on live specimens.

Objectivelens function

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Objectivelens magnification

Numerical aperture NA denotes the light acceptance angle. Where θ is the maximum 1/2 acceptance ray angle of the objective and n is the index of refraction of the immersive medium, the NA can be denoted by

The parfocal length of a microscope is defined as the distance between the object being studied and the objective mounting plane.

The field of view (FOV) of a microscope is simply the area of the object that can be imaged at any given time. For an infinity-corrected objective, this will be determined by the objective magnification and focal length of the tube lens. Where a camera is used the FOV  also depends on sensor size.

Low powerobjectivelens

A week ago, I co-hosted a live Zoom webinar in honor of Women’s History Month.  I practiced the speaker introductions without notes but changed course at the last minute. I chose to read from the teleprompter on my computer screen—with my reading glasses.

The optical performance of an objective is dependent largely on the optical aberration correction, and these corrections are also central to image quality and measurement accuracy. Objective lenses are classified as achromat, plan achromat, plan semi apochromat, plan apochromat, and super apochromat depending on the degree of correction.

Almost all photographs are a record of reflections. A webcam is no different.  The camera captures light reflecting off a surface.  In photography it is called the Angle of Incidence. When you grasp and practice the general concept, you will be able to angle the light source to reduce the glare.  If the light source is sunlight from a window, you may need to lower the shades or block the light with cardboard.

Here are some proven tips to avoid excessive glare on your glasses when on video calls. Note the word excessive: unless you wear fashion frames without lenses or work out of a video production studio, some reflection is near impossible to avoid, particularly when working remotely.

While most microscope objectives are designed to work with air between the objective and cover glass, objectives lenses designed for higher NA and greater magnification sometimes use an alternate immersion medium. For instance, a typical oil immersion object is meant to be used with an oil with refractive index of 1.51.

Objectivelens microscope function

Most microscopes rely on background illumination such as daylight or a lightbulb rather than a dedicated light source. In brightfield illumination (also known as Koehler illumination), two convex lenses, a collector lens and a condenser lens,  are placed so as to saturate the specimen with external light admitted into the microscope from behind. This provides a bright, even, steady light throughout the system.

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A basic achromatic objective is a refractive objective that consists of just an achromatic lens and a meniscus lens, mounted within appropriate housing. The design is meant to limit the effects of chromatic and spherical aberration  as they bring two wavelengths of light to focus in the same plane. Plan Apochromat objectives can be much more complex with up to fifteen elements. They can be quite expensive, as would be expected from their complexity.

An microscope objective  may be either reflective or refractive. It may also be either finite conjugate or infinite conjugate.

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The working distance of a microscope is defined as the free distance between the objective lens and the object being studied. Low magnification objective lenses have a long working distance.

At Avantier we produce high quality microscope objectives lenses, ocular lenses, and other imaging systems. We are also able to provide custom designed optical lenses as needed. Chromatic focus shift, working distance, image quality, lens mount, field of view, and antireflective coatings are just a few of the parameters we can work with to create an ideal objective for your application. Contact us today to learn more about how we can help you meet your goals.

The computer screen is a big source of glare, particularly at night.  Dim the screen or attach an anti-glare monitor filter to your display.  At night, increase the level of ambient room light to offset the intensity of the glare coming from the computer screen and your video conferencing lighting.

The eyepiece or ocular lens is the part of the microscope closest to your eye when you bend over to look at a specimen. An eyepiece usually consists of two lenses: a field lens and an eye lens. If a larger field of view is required, a more complex eyepiece  that increases the field of view can be used instead.

A microscope is an optical device designed to magnify the image of an object, enabling details indiscernible to the human eye to be differentiated. A microscope may project the image onto the human eye or onto a camera or video device.

Objectivelens telescope

Immediately after the show, an email arrived in my inbox from a world-class public speaker and friend with the gentle warning, “I think you should know…”  Attached was a screenshot of me with a halo reflected on each lens of my glasses.

One of the keys to looking good on a video call is to practice and test.  Experiment with different light configurations.  Record yourself in a variety of setups and find what works so that you can show up as your absolute best.  If you are still having trouble managing your glasses on the screen, you may want to consider making the move to contacts.

Types ofobjectivelenses

Refractive objectives are so-called because the elements bend or refract light as it passes through the system. They are well suited to machine vision applications, as they can provide high resolution imaging of very small objects or ultra fine details. Each element within a refractive element is typically coated with an anti-reflective coating.

A basic compound microscope could consist of just two elements acting in relay, the objective and the eyepiece. The objective relays a real image to the eyepiece, while magnifying that image anywhere from 4-100x.  The eyepiece magnifies the real image received typically by another 10x, and conveys a virtual image to the sensor.

Ring lights cast shadows and require lots of fill, not to mention the reflection they cast on your glasses or any reflective surface in your shot.  Dimmable LED Panel lights emit softer light that make reflections less noticeable than the telltale circle.   A broad light source like a Softbox lighting  kit is another affordable solution for studio quality lighting at home.

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Although today’s microscopes are usually far more powerful than the microscopes used historically, they are used for much the same purpose: viewing objects that would otherwise be indiscernible to the human eye.  Here we’ll start with a basic compound microscope and go on to explore the components and function of larger more complex microscopes. We’ll also take an in-depth look at one of the key parts of a microscope, the objective lens.

I teach business leaders how to up-level their presence and authority on Zoom, yet I was not  practicing what I preach.  Instead, I opted for the comfort of being able to read without squinting, even with 24-point type.

While a magnifying glass consists of just one lens element and can magnify any element placed within its focal length, a compound lens, by definition, contains multiple lens elements. A relay lens system is used to convey the image of the object to the eye or, in some cases, to camera and video sensors.

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Both the objective lens and the eyepiece also contribute to the overall magnification of the system. If an objective lens magnifies the object by 10x and the eyepiece by 2x, the microscope will magnify the object by 20. If the microscope lens magnifies the object by 10x and the eyepiece by 10x, the microscope will magnify the object by 100x. This multiplicative relationship is the key to the power of microscopes, and the prime reason they perform so much better than simply magnifying glasses.

There are two major specifications for a microscope: the magnification power and the resolution. The magnification tells us how much larger the image is made to appear. The resolution tells us how far away two points must be to  be distinguishable. The smaller the resolution, the larger the resolving power of the microscope. The highest resolution you can get with a light microscope is 0.2 microns (0.2 microns), but this depends on the quality of both the objective and eyepiece.

A reflective objective works by reflecting light rather than bending it. Primary and secondary mirror systems both magnify and relay the image of the object being studied. While reflective objectives are not as widely used as refractive objectives, they offer many benefits. They can work deeper in the UV or IR spectral regions, and they are not plagued with the same aberrations as refractive objectives. As a result, they tend to offer better resolving power.

Do not look at the light.  Lower your chin slightly but still make eye-level contact with the camera.  You can also  lift the earpieces (the temples) a notch to increase the angle of the lenses to the light source.

Objectivelens microscope

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Historically microscopes were simple devices composed of two elements. Like a magnifying glass today, they produced a larger image of an object placed within the field of view. Today, microscopes are usually complex assemblies that include an array of lenses, filters, polarizers, and beamsplitters. Illumination is arranged to provide enough light for a clear image, and sensors are used to ‘see’ the object.