Numerical apertureof 40x objective

…microscope are determined by the numerical aperture (N.A.) of the objective. The N.A. is defined as the sine of half the angle of the cone of light from each point of the object that can be accepted by the objective multiplied by the refractive index (R.I.) of the medium in…

…a microscope is called the numerical aperture (NA) and is equal to the sine of half the angle subtended by the aperture at an object point times the index of refraction of the medium between the object and the objective lens. For binoculars, telescopes, and photographic lenses in which the…

Numerical apertureofmicroscopeformula

He designated the term numerical aperture (N.A.) as the measure of the objective’s ability to collect diffracted light and thus also of its power to resolve detail. On this basis it is obvious that the greater the magnification of the objective, the greater the required N.A. of the objective.…