The next number we need to determine is the diagonal measurement of the monitor in mm (3). Typically a monitor's measurement is provided in inches. We will just simply convert the inches measurement to mm. For example, if you are using a 19" monitor, multiply this number by 25.4 to convert it to mm. 19" x 25.4 = 482.6mm diagonal monitor measurement.

Now we can find the total on-screen magnification by multiplying optical magnification x digital magnification. In our example 2 x 60.325 = 120.65x on-screen magnification.

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Coma aberration in physics is caused by off-axis point sources such as elements of a lens or mirror being misaligned. The effect is distortion in the image, ...

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A: The index of refraction for water is 1.33 and the index of refraction of glass is about 1.55 so the index of refraction of water is less than the index of refraction of glass. This is the same situation we had in the example oflight passing from air into water. So light will be bent toward the normal.

Light behaves in much the same way as it moves from air, where it travels very fast, into another medium like glass or plastic or water, where it travel more slowly.

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The microscope c-mount adapter (2) is the piece used to connect the microscope and the microscope camera. The c-mount adapter will have a number printed on the side of it. In the image at right, the c-mount adapter has a 0.5x lens built into it.

A common question when viewing a microscopy image on a large screen is: What is the total magnification I am viewing on my monitor?" In order to correctly calculate the answer to this question, we must know the answer to the following four questions.

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The microscope objective lens magnification is printed on the side of the lens as shown in the image at left (1). If you are using a stereo microscope the objective lens value would be printed on the zoom knob or on the objective turret that is turned to change the microscope magnification.

The final number we will need in order to determine the on-screen magnification is the size of the camera sensor in mm (4). The size of the sensor differs slightly from the size of the chip, so the best way to find this number is to use the chart located below. The microscope camera sensor sizes are listed at the top of the chart, and the corresponding diagonal sensor size in mm is shown below.

If we are using the 19" monitor we mentioned earlier, to convert this to mm we multiple 19 x 25.4 = 482.6mm screen size.  For this example, let's say we are using a microscope camera with a 1/2" camera sensor in it. Based on the chart above we would calculate digital magnification by using 482.6mm / 8.00 = 60.325.

If you have questions regarding calculating on-screen microscope magnification contact Microscope World and we will be happy to help.

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Reflection Total Internal Reflection Back to "Reflection and Refraction" Page (C) 2003, Doug Davis; all rights reserved

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This bending of light is known as refraction. It is due to a difference in the speed of light in the two media. Instead of describing the speed of light directly, we usually talk about the index of refraction and label that as "n". The index of refraction is the ratio of the speed of light in vacuum, c, to the speed of light in a particular medium, v. That means

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Think of a marching band that travels across a smooth concrete parking lot onto a rough and muddy grassy playing field. The band members can take larger steps and move faster on the concrete than on the grass. Yet, being band members, they all remain in step, taking steps at the same pace whether on concrete or grass. This causes the band to change its direction as it moves from the faster concrete to the slower grass.

Q: What is the speed of light in a glass whose index of refraction is 1.60? A: The index of refraction of a medium is n = c/v. We can multiply both sides by v and divide both sides by n to get v = c/n Now we can use numbers to calculate v, the speed of light in the glass, v = ( 3.0 x 108 m/s ) / ( 1.6 ) v = 1.88 x 108 m/s Reflection Total Internal Reflection Back to "Reflection and Refraction" Page (C) 2003, Doug Davis; all rights reserved

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Q: If light travels from water into glass, will it be bent toward or away from the normal? A: The index of refraction for water is 1.33 and the index of refraction of glass is about 1.55 so the index of refraction of water is less than the index of refraction of glass. This is the same situation we had in the example oflight passing from air into water. So light will be bent toward the normal. Q: What is the speed of light in a glass whose index of refraction is 1.60? A: The index of refraction of a medium is n = c/v. We can multiply both sides by v and divide both sides by n to get v = c/n Now we can use numbers to calculate v, the speed of light in the glass, v = ( 3.0 x 108 m/s ) / ( 1.6 ) v = 1.88 x 108 m/s Reflection Total Internal Reflection Back to "Reflection and Refraction" Page (C) 2003, Doug Davis; all rights reserved