Refractive index of glass

Now suppose that the rays of light are traveling through the focal point on the way to the lens. These rays of light will refract when they enter the lens and refract when they leave the lens. As the light rays enter into the more dense lens material, they refract towards the normal; and as they exit into the less dense air, they refract away from the normal. These specific rays will exit the lens traveling parallel to the principal axis.

Some microscopes include extra low power 1x or 2x objectives for an even wider field of view to help orient the largest samples. These have become more common on inverted microscopes.

Phase contrast and fluorescence microscopy require specialized objectives with matched condenser optics to image transparent specimens. These are often incorporated as a fourth objective or replace one of the standard ones.

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The range of magnifications enables users to choose the appropriate level for their particular application, whether surveying tissue architecture or examining subcellular organelles. No single objective lens can provide optimal performance across this wide range of viewing needs.

First lets consider a double convex lens. Suppose that several rays of light approach the lens; and suppose that these rays of light are traveling parallel to the principal axis. Upon reaching the front face of the lens, each ray of light will refract towards the normal to the surface. At this boundary, the light ray is passing from air into a more dense medium (usually plastic or glass). Since the light ray is passing from a medium in which it travels fast (less optically dense) into a medium in which it travels relatively slow (more optically dense), it will bend towards the normal line. This is the FST principle of refraction. This is shown for two incident rays on the diagram below. Once the light ray refracts across the boundary and enters the lens, it travels in a straight line until it reaches the back face of the lens. At this boundary, each ray of light will refract away from the normal to the surface. Since the light ray is passing from a medium in which it travels slow (more optically dense) to a medium in which it travels fast (less optically dense), it will bend away from the normal line; this is the SFA principle of refraction.

The compound light microscope is an indispensable tool used ubiquitously in science disciplines to visualize small objects in fine detail. Unlike simple magnifying glasses, the compound microscope uses two lens systems to enlarge specimens up to 1000x their actual size.

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While the basic 3 objective arrangement still dominates today, some microscopes incorporate additional objectives or special enhancements for increased performance and capabilities.

Lenses with lower power and larger fields of view can have optics optimized for brightness whereas high magnification lenses with narrow fields are optimized for resolution at the expense of brightness.

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Refractionexamples

Now we have three incident rays whose refractive behavior is easily predicted. These three rays lead to our three rules of refraction for converging and diverging lenses. These three rules are summarized below.

The set of 3 objective lenses on most compound microscopes elegantly fulfills the range of observational needs in microscopy, from scanning the big picture to examining the most minute details. Their differing optical properties and fields of view provide efficient and flexible viewing capabilities not possible with a single objective lens. The specific numbers and powers may be tailored for particular applications, but the core triad arrangement remains ubiquitous out of logical necessity.

Now let's investigate the refraction of light by double concave lens. Suppose that several rays of light approach the lens; and suppose that these rays of light are traveling parallel to the principal axis. Upon reaching the front face of the lens, each ray of light will refract towards the normal to the surface. At this boundary, the light ray is passing from air into a more dense medium (usually plastic or glass). Since the light ray is passing from a medium in which it travels relatively fast (less optically dense) into a medium in which it travels relatively slow (more optically dense), it will bend towards the normal line. This is the FST principle of refraction. This is shown for two incident rays on the diagram below. Once the light ray refracts across the boundary and enters the lens, it travels in a straight line until it reaches the back face of the lens. At this boundary, each ray of light will refract away from the normal to the surface. Since the light ray is passing from a medium in which it travels relatively slow (more optically dense) to a medium in which it travels fast (less optically dense), it will bend away from the normal line. This is the SFA principle of refraction. These principles of refraction are identical to what was observed for the double convex lens above.

The lowest magnification objective is typically a 4x or 10x lens. Its primary purpose is to provide a wide field of view of the overall specimen on the slide for initial orientation and scanning. The low magnification reduces aberrations from optical imperfections.

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The provision of 3 objective lenses with differing optical properties confers important complementary advantages that enhance the microscopy user experience and workflow efficiency.

Certain instruments are designed to accommodate additional high-power 60x or 100x objective lenses when extremely high magnification and resolution are critical, such as for cytology or microbiology applications.

We have already learned that a lens is a carefully ground or molded piece of transparent material that refracts light rays in such a way as to form an image. Lenses serve to refract light at each boundary. As a ray of light enters a lens, it is refracted; and as the same ray of light exits the lens, it is refracted again. The net effect of the refraction of light at these two boundaries is that the light ray has changed directions. Because of the special geometric shape of a lens, the light rays are refracted such that they form images. Before we approach the topic of image formation, we will investigate the refractive ability of converging and diverging lenses.

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Optical refractionpdf

The standard compound light microscope has 3 objective lenses to provide different magnification powers, resolving abilities, and fields of view to visualize specimens in increasing detail.

The above diagram shows the behavior of two incident rays approaching parallel to the principal axis of the double concave lens. Just like the double convex lens above, light bends towards the normal when entering and away from the normal when exiting the lens. Yet, because of the different shape of the double concave lens, these incident rays are not converged to a point upon refraction through the lens. Rather, these incident rays diverge upon refracting through the lens. For this reason, a double concave lens can never produce a real image. Double concave lenses produce images that are virtual. This will be discussed in more detail in the next part of Lesson 5. If the refracted rays are extended backwards behind the lens, an important observation is made. The extension of the refracted rays will intersect at a point. This point is known as the focal point. Notice that a diverging lens such as this double concave lens does not really focus the incident light rays that are parallel to the principal axis; rather, it diverges these light rays. For this reason, a diverging lens is said to have a negative focal length.

What is refractive index

Any incident ray traveling parallel to the principal axis of a diverging lens will refract through the lens and travel in line with the focal point (i.e., in a direction such that its extension will pass through the focal point).

The above diagram shows the behavior of two incident rays traveling through the focal point on the way to the lens. Note that the two rays refract parallel to the principal axis. A second generalization for the refraction of light by a double convex lens can be added to the first generalization.

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Optical refractiontest

These three rules of refraction for converging and diverging lenses will be applied through the remainder of this lesson. The rules merely describe the behavior of three specific incident rays. While there is a multitude of light rays being captured and refracted by a lens, only two rays are needed in order to determine the image location. So as we proceed with this lesson, pick your favorite two rules (usually, the ones that are easiest to remember) and apply them to the construction of ray diagrams and the determination of the image location and characteristics.

Angle ofrefraction

Now suppose that the rays of light are traveling towards the focal point on the way to the lens. Because of the negative focal length for double concave lenses, the light rays will head towards the focal point on the opposite side of the lens. These rays will actually reach the lens before they reach the focal point. These rays of light will refract when they enter the lens and refract when they leave the lens. As the light rays enter into the more dense lens material, they refract towards the normal; and as they exit into the less dense air, they refract away from the normal. These specific rays will exit the lens traveling parallel to the principal axis.

The 10x or 20x medium power objective delivers comfortable viewing magnification and reasonably high resolution to see some finer details in the context of the larger specimen structure. It is commonly used for routine examination, counting cells, measuring proportions, and making sketches.

Higher magnification requires higher resolution to realize the full benefit. The higher-powered objectives have correspondingly greater resolving power to take advantage of the increased magnification whereas the lower-power lenses have comparatively less resolution which is ample for their magnification level.

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The above discussion focuses on the manner in which converging and diverging lenses refract incident rays that are traveling parallel to the principal axis or are traveling through (or towards) the focal point. But these are not the only two possible incident rays. There are a multitude of incident rays that strike the lens and refract in a variety of ways. Yet, there are three specific rays that behave in a very predictable manner. The third ray that we will investigate is the ray that passes through the precise center of the lens - through the point where the principal axis and the vertical axis intersect. This ray will refract as it enters and refract as it exits the lens, but the net effect of this dual refraction is that the path of the light ray is not changed. For a thin lens, the refracted ray is traveling in the same direction as the incident ray and is approximately in line with it. The behavior of this third incident ray is depicted in the diagram below.

The multiple objectives with parcentered optics allow users to quickly switch between lenses and magnifications to obtain just the right view. This facilitates efficient and intuitive workflows.

Refracted ray

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Practically, low magnification facilitates efficient scanning of the overall specimen to find areas of interest to study further, saving significant time compared to searching blindly at high power. It provides necessary contextual orientation.

Proper illumination from below is vital for viewing clarity. The maximum resolution or resolving power is limited by the wavelength of light and optics. Higher quality objectives provide greater usable resolution to see fine details.

The major components of a compound microscope are the ocular lens in the eyepiece, the objective turret housing multiple objective lenses, the condenser lens below the stage, the illumination system, and the mechanical arm. Each part plays a critical optical or functional role.

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5 applications ofrefractionof light

The standard compound microscope contains 3 objective lenses with different powers, resolutions, and fields of view to provide a tiered viewing experience.

The level of microscope magnification depends on the optical properties of both the ocular and objective lenses. The ocular lens magnifies the primary image 10x. The objectives provide progressively higher magnifying power of 4x, 10x, 40x, and sometimes 100x.

These two "rules" will greatly simplify the task of determining the image location for objects placed in front of converging lenses. This topic will be discussed in the next part of Lesson 5. For now, internalize the meaning of the rules and be prepared to use them. As the rules are applied in the construction of ray diagrams, do not forget the fact that Snells' Law of refraction of light holds for each of these rays. It just so happens that geometrically, when Snell's Law is applied for rays that strike the lens in the manner described above, they will refract in close approximation with these two rules. The tendency of incident light rays to follow these rules is increased for lenses that are thin. For such thin lenses, the path of the light through the lens itself contributes very little to the overall change in the direction of the light rays. We will use this so-called thin-lens approximation in this unit. Furthermore, to simplify the construction of ray diagrams, we will avoid refracting each light ray twice - upon entering and emerging from the lens. Instead, we will continue the incident ray to the vertical axis of the lens and refract the light at that point. For thin lenses, this simplification will produce the same result as if we were refracting the light twice.

Having a continuum of magnifications allows the microscope to accommodate samples of vastly different sizes from whole insect bodies down to single cells. A single high-power objective cannot cover this entire range.

High-performance objectives may have adjustable correction collars to optimize the optical correction for viewing specimen slides with different coverslip thicknesses, allowing the best possible image.

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The above diagram shows the behavior of two incident rays approaching parallel to the principal axis. Note that the two rays converge at a point; this point is known as the focal point of the lens. The first generalization that can be made for the refraction of light by a double convex lens is as follows:

A question commonly asked about compound microscopes is: What’s the purpose of having 3 objective lenses attached to it? The answer is quite simple.

The above diagram shows the behavior of two incident rays traveling towards the focal point on the way to the lens. Note that the two rays refract parallel to the principal axis. A second generalization for the refraction of light by a double concave lens can be added to the first generalization.

Any incident ray traveling parallel to the principal axis of a converging lens will refract through the lens and travel through the focal point on the opposite side of the lens.

The 40x or 100x high power objective produces the highest magnification and resolution to reveal subcellular structures and other intricate details not discernable with the lower powered lenses but has an extremely narrow field of view. It is used for critical inspection of key areas after initial surveys with lower-powered objectives.