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Compoundmicroscope objective lens magnification

3. Once you've focused using the scanning objective, switch to the low power objective (10x). Use the coarse knob to refocus and move the mechanical stage to re-center your image. Again, if you haven't focused on this level, you will not be able to move to the next level.

Objective lens microscopefunction

Your microscope has 4 objective lenses: Scanning (4x), Low (10x), High (40x), and Oil Immersion (100x). In this lab, you will not use the oil immersion lens; it is for viewing microorganisms and requires technical instructions not covered in this procedure.

The difference between eyepiece and objective lens in optical microscope The eyepiece and objective of a microscope are both convex lenses, with the difference being that the focal length of the eyepiece is shorter,

Types ofobjectivelenses

1. Place the letter “e” slide onto the mechanical stage. Be sure to note the orientation of the letter “e” as it appears to your naked eye.

Low powerobjective magnification

4. Switch to low power (10x). This may be sufficient to view your chosen organism. Try to note how it moves and do your best to draw it as you see it, unless you need more magnification.

1. Using the transfer pipette, transfer a drop of pond water onto a microscope slide. The best specimens usually come from the bottom and probably will contain chunks of algae or other debris that you can see with your naked eye.

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2. Always start with the stage as low as possible and using scanning objective (4x). Odds are, you will be able to see something on this setting (sometimes it’s only a color). Use the coarse knob to focus: the image may be small at this magnification, but you won't be able to find it on the higher powers without this first step. Move the mechanical stage until your focused image is also centered.

In addition to the objective lenses, the ocular lens (eyepiece) has a magnification. The total magnification is determined by multiplying the magnification of the ocular and objective lenses.

High powerobjective lens

Microlens arrays are rigid arrangements of very tiny lenses, each with a diameter of less than 10 millimeters. These arrays may be one or two dimensional, and ...

In this lab, parts of the microscope will be reviewed. Students will learn the proper use and care of the microscope and observe samples from pond water.

What isobjective lensinmicroscope

This portion of the procedure is another practice to demonstrate depth perception. Many new microscope users find it difficult to conceive that the specimen on the slide is in three dimensions. As the stage is moved up and down, different threads will be in focus.

A large part of the learning process of microscopy is getting used to the orientation of images viewed through the oculars as opposed to with the naked eye. A common mistake is moving the mechanical stage the wrong way to find the specimen. This procedure is merely practice designed to make new users more comfortable with using the microscope.

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Eyepiece: Installed at the upper end of the lens barrel, usually with 2-3 pieces, engraved with symbols of 5 ×, 10 ×, or 15 × to indicate its magnification, usually equipped with a 10 × eyepiece Objective lens: Installed on the rotator at the lower end of the lens barrel, there are usually 3-4 objective lenses, among which the shortest one engraved with the "10 ×" symbol is the low magnification lens, the longer one engraved with the "40 ×" symbol is the high magnification lens, and the longest one engraved with the "100 ×" symbol is the oil lens. In addition, a circle of different colored lines is often added to distinguish between the high magnification lens and the oil lens

High powerobjective microscopefunction

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Low powerobjective lens

5. Once you have centered and focused the image, switch to high power (40x) and refocus. Note movements and draw the organism as you see it.

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1. The function of the eyepiece of an optical microscope It is to further enlarge the real image that has been magnified by the objective lens and has a clear resolution, to the extent that the human eye can easily distinguish it.

The objective lens is equivalent to the lens of a projector, and objects are inverted and magnified into a real image through the objective lens; The eyepiece is equivalent to a regular magnifying glass, and the real image formed by the objective lens is then transformed into an upright and magnified virtual image through the eyepiece

2. Use the SCANNING (4x) objective and course focus adjustment to focus, then move the mechanical stage around to find the letter “e”. Note the orientation when viewed through the oculars.

all of the points of the physical environment that can be perceived by a stable eye at a given moment.

2. The relationship between the eyepiece and objective lens of an optical microscope. The objective lens has already distinguished the fine structures clearly. If it is not magnified by the eyepiece, it cannot reach the size that the human eye can distinguish, and then it cannot be seen clearly; However, fine structures that cannot be distinguished by the objective lens cannot be seen clearly even after being magnified by a high-power eyepiece. Therefore, the eyepiece can only serve as a magnifying lens and will not improve the resolution of the microscope. Sometimes, although the objective lens can distinguish two objects that are very close together, the distance between the images of these two objects is smaller than the resolution distance of the eye, so it is still difficult to see clearly. So, the eyepiece and objective lens are both interrelated and mutually restrictive.

2. Use the SCANNING (4x) objective and course focus adjustment to focus, then move the mechanical stage around to find the threads.

3. Use the SCANNING (4x) objective to focus, then move the mechanical stage around to scan the slide for live microorganisms. You are looking for tiny swimming beings- they may look green or clear and might be very small. Choose one to focus on and center it in your visual field.

A microscope is an instrument that magnifies an object so that it may be seen by the observer. Because cells are usually too small to see with the naked eye, a microscope is an essential tool in the field of biology. In addition to magnification, microscopes also provide resolution, which is the ability to distinguish two nearby objects as separate. A combination of magnification and resolution is necessary to clearly view specimens under the microscope. The light microscope bends a beam of light at the specimen using a series of lenses to provide a clear image of the specimen to the observer.

This page titled 1.4: Microscopy is shared under a CC BY-SA 4.0 license and was authored, remixed, and/or curated by Susan Burran and David DesRochers (GALILEO Open Learning Materials) via source content that was edited to the style and standards of the LibreTexts platform.

The magnification of the eyepiece is inversely proportional to its length, while the magnification of the objective lens is directly proportional to its length.

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