Microscope

by D San Martín · 2010 · Cited by 31 — This work shows that, under these conditions, the use of Nomarski differential interference contrast microscopy under bright field illumination helps to ...

In this image you can see how a shallow depth of field keeps the focus on the action. Photo by Samuel King In this image you can see how a shallow depth of field keeps the focus on the action. Download Image  Share Image: X Facebook Email Photo by: Samuel King VIRIN: 170908-F-OC707-0517C

The following graphic illustrates how changing these factors: aperture, focal length and the distance from the subject affect the depth of field.

You can affect the depth of field by changing the following factors: aperture, the focal length and the distance from the subject.

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Microscopic objective is an aberration cancelling system. This means that for a pair of conjugate points on the axis, when the aberration is eliminated and the sine condition is achieved, each objective only has two such aberration cancellations. Therefore, any change in the calculated position of the object and image results in an increase in aberration. On the rotator installed at the lower end of the lens barrel, there are generally 3-4 objective lenses, among which the shortest one engraved with the "10 ×" symbol is a low magnification lens, the longer one engraved with the "40 ×" symbol is a high magnification lens, and the longest one engraved with the "100 ×" symbol is an oil lens. In addition, a circle of different colors is often added to the high magnification lens and the oil lens to show the difference.

Infographic illustrates how changing the aperture, the focal length and the distance from the subject affect the depth of field. Download Image  Share Image: X Facebook Email Photo by: DINFOS PAVILION Team VIRIN: 200907-D-PA656-0002

Objectiveback focal plane

Depth of field (DoF) is the area between the nearest and farthest points from the camera that are acceptably sharp in an image. A deep DoF means all or most of your photo will be in focus, including the foreground, subject and background. Use a deep DoF in group photos, landscape shots and when elements in the background or foreground add to the message the photo is attempting to communicate. A shallow DoF means more narrow range will be acceptably sharp in the image. Shallow DoF is good to use when you want to isolate your subject from their surroundings, such as in a portrait or when elements in the background or foreground may be distracting.

The structure of the objective lens is complex and precise, usually composed of a combination of lenses, with each lens spaced at a certain distance to reduce the difference. Each group of lenses is made by bonding one or several lenses with different materials and parameters. There are many specific requirements for the objective lens, such as axial alignment and alignment. The modern microscope objective has reached a high level of perfection, with its numerical aperture approaching its limit, and the difference between the resolution at the center of the field of view and the theoretical value is negligible. However, the possibility of further increasing the field of view of the microscope objective and improving the imaging quality of the field edge still exists, and this research work is still ongoing to this day.

In this image a medium depth of field allows the viewer to focus on multiple subjects without creating confusion for your eyes Photo by Sebastian J. Sciotti Jr. In this image a medium depth of field allows the viewer to focus on multiple subjects without creating confusion for your eyes Download Image  Share Image: X Facebook Email Photo by: Sebastian J. Sciotti Jr. VIRIN: 170525-D-SS007-019C

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The aperture is the opening created by a set of overlapping metal blades, known as the diaphragm, inside a photographic lens. This opening controls the amount of light coming through the lens. The wider the aperture, the less depth of field you capture. The smaller the aperture, the deeper the depth of field.

Microscopic objective and eyepiece differ in their involvement in imaging. Objective is the most complex and important part of a microscope, operating in a wide beam of light (with a large aperture), but these beams have a smaller inclination angle to the optical axis (with a smaller field of view); The eyepiece works in a narrow beam of light, but its inclination angle is large (with a large field of view). When calculating the objective and eyepiece, there is a significant difference in eliminating aberration.

Aligning focus is not only used in microscopy, but also when observing images clearly with a certain magnification objective, and when switching to another magnification objective, the imaging should be basically clear, and the center deviation of the image should also be within a certain range, that is, the degree of axis alignment. The quality of confocal performance and the degree of alignment are important indicators of microscope quality, which are related to the quality of the objective lens itself and the accuracy of the objective lens converter.

Distance to subject refers to the length between the camera and the focus of the image. The closer the camera is to the subject it is focusing on, the narrower the depth of field will be. Inversely, the farther away the subject is from the camera, the wider the depth of field will be.

Objective lens

by C Hu · 2018 · Cited by 52 — The reflectivity in the infrared region depends mainly on the intraband transition and is closely related to the plasma energy. The Ag-doping- ...

The aberration related to a wide beam of light is spherical aberration, coma aberration, and positional chromatic aberration; The aberrations related to the field of view are astigmatism, field curvature, distortion, and magnification aberration.

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The objective lens is the most important optical component of a microscope, which uses light to create the first image of the object being tested. Therefore, it directly affects and affects the quality of the image and various optical technical parameters, and is the primary standard for measuring the quality of a microscope.

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In this image a deep depth of field allows the viewer to take in many subjects, including an artillery shell mid-flight. Photo by Staff Sgt. Steven Schneider In this image a deep depth of field allows the viewer to take in many subjects, including an artillery shell mid-flight. Download Image  Share Image: X Facebook Email Photo by: Staff Sgt. Steven Schneider VIRIN: 170918-O-N0132-7230C

The focal length of the lens determines the image magnification. The wider the lens, the shorter the focal length. This allows you to capture a wider depth of field. The longer or more zoomed in the camera lens, the less depth of field you capture.

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Typically a lower magnification objective lens will have a larger field of view and lower resolution, and a higher magnification objective lens will have a ...

There are limits to the practicality of a high-powered magnifying glass ... lens quality (distortion, color clarity, scratch-resistance) ... Although the perfect ...