For example, if you wanted to view a whole penny with a digital microscope, the penny has a diameter of 19mm, so you will need a minimum of 19mm field of view to see the entire penny on the monitor screen. If you wanted to focus on the letter “G” in the word “God” on the penny, then you will need at least 1mm field of view. Examining a 1mm feature in 19mm field of view, while doable, may not realistic for a close inspection of the details: 1mm would only take up 5% (1mm feature divided by 19mm FOV) of the monitor screen space. If the field of view was decreased to 4mm, then the 1mm feature would now fill up 25% of the monitor screen, allowing better viewing of the finer details on the “G”.Since applications often require examining features in a variety of sizes, we often ask what your field of view range requirement is for your application. Continuing with the penny example, in order to examine both the letter “G” in detail and the penny in its entirety, it would be recommended to choose a digital microscope with at least 4mm to 19mm FOV range.

Note that calculating magnification in telescopes uses a different equation than calculating magnification in microscopes. For telescopes, one magnification calculation uses the focal lengths of the telescope and the eyepiece. That calculation is:

Magnification ofcompoundmicroscopeformula

With many high powered microscopes the magnification of an object might reach resolutions up to micrometers (one micrometer in metric is around 40 millionths of an inch in imperial).

Blaettler, Karen G. "How To Calculate Total Magnification Of A Microscope Or Telescope" sciencing.com, https://www.sciencing.com/calculate-total-magnification-5062733/. 8 April 2023.

Blaettler, Karen G. (2023, April 8). How To Calculate Total Magnification Of A Microscope Or Telescope. sciencing.com. Retrieved from https://www.sciencing.com/calculate-total-magnification-5062733/

Microscopes use lenses to magnify objects. A simple microscope uses only one lens; a magnifying glass could be called a simple microscope. The magnification of a simple microscope doesn't need any calculation because the single lens is usually labeled. A hand-lens, for example, might be labeled with 10x, meaning the lens magnifies the object to look ten times larger than the actual size.

Since the field of view remains a consistent, real dimension regardless of monitor size, we are able to use FOV to narrow down which digital microscope model may work best for your application requirements before we start finalizing the microscope configuration. Contact us today for assistance on finding the right digital microscope for our needs!

Magnificationformula Biology

The field of view, by definition, fills the entire monitor screen, and is not affected by the monitor size used. A digital microscope’s FOV range remains constant, but video magnification will change according to your monitor size. On the HD801, the smallest FOV that can be achieved is 0.2” at 4.5” working distance (2nd row on chart), regardless of monitor size. However, the smallest 0.2” FOV equates to a higher magnification when the digital microscope is connected to a 24” monitor (122x) than it would be if viewed on a 12” monitor (59x).

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Microscope magnificationCalculator

Microscopes magnify the tiniest inhabitants of this world. From the minute details of cells to the delicate cilia of paramecium to the intricate workings of Daphnia, microscopes reveal many minuscule secrets. Calculating total magnification power uses simple observation and basic multiplication. As the magnification increases, the field of view decreases. This is important to keep in mind when trying to observe larger objects with greater detail.

The total magnification of 40 means that the object appears forty times larger than the actual object. If the viewer changes to the 10x objective lens, the total magnification will be the ocular's 10x magnification multiplied by the new objective lens's 10x magnification, calculated as:

To demonstrate how that can be done, we will use our HD801 HD 1080p digital microscope’s viewing chart as a reference point. The HD801’s chart provides diagonal field of view values, which refers to the area of the size shown on screen, measured diagonally from one corner of the monitor to its opposite corner (D). FOV can be specified as diagonal, horizontal or vertical values.

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Once the magnification of each individual lens is known, calculating total magnification is simple math. Multiply the magnification of the lenses together. For example, if the eyepiece magnification is 10x and the objective lens in use has a magnification of 4x, the total magnification is:

Ocular lensmagnification

(Mobile: swipe left for horizontal scrolling)Working DistanceField of View (Diagonal)Video Mag. 12" MonitorVideo Mag. 24" MonitorLow Mag.High Mag.Low Mag.High Mag.Low Mag.High Mag.4.5" (114 mm)1.2"0.2"9x59x20x122x30 mm5 mm5" (127 mm)1.4"0.2"8x48x16x100x36 mm6.1 mm6" (152 mm)1.9"0.3"6x36x13x75x47 mm8.1 mm8" (203 mm)2.6"0.5"4x26x9x53x65 mm11.5 mm10" (254 mm)3.3"0.6"3x20x7x41x85 mm15 mm12" (305 mm)4.1"0.7"2x16x5x34x103 mm18 mm

Totalmagnification of40x

Microscopes are used to enlarge the image of your object or specimen, so you would assume magnification ranges should be used as specification requirement when shopping for a new microscope. However, determining magnification on a traditional microscope differs from the way it is calculated on a digital microscope, so it would be not be comparing apples to apples without some hand calculations. Instead, field of view should be used as the quantitative value to determine which digital microscopes will work best for your application.

On a purely digital microscope, not only are there no eyepieces, there often will not be any objective lenses either to calculate optical magnification with. Instead, video magnification on a digital microscope is relative to the size of the monitor you are using with the microscope. For that reason, it is easier to refer to the field of view as the defining value to determine if a particular digital microscope model suits your application’s needs. The field of view (FOV) is the size of area that the digital microscope’s camera is seeing and displaying on the monitor screen. FOV, because it is a number representing a real dimension, can be easily determined just by knowing how big of an area you need to be inspecting.

Blaettler, Karen G. How To Calculate Total Magnification Of A Microscope Or Telescope last modified April 8, 2023. https://www.sciencing.com/calculate-total-magnification-5062733/

Compound microscopes use two or more lenses to magnify the specimen. The standard school microscope combines two lenses, the ocular and one objective lens, to magnify the object. The ocular or eyepiece is found at the top of the body tube. The objective lens points down toward the object to be magnified. Most microscopes have three or four objective lenses mounted on a rotating nosepiece. Rotating the nosepiece lets the viewer change the magnification. Different objective lenses provide different magnification options. The objective lens focuses light to the ocular lens (or eyepiece) which is used to view the specimens. Compound light microscopes often include various objective lenses labeled with the magnification of the objective lens, and the power of the eyepiece can often be found on the ocular lens.

Totalmagnification of microscope

Let’s first take a step back: how is magnification (referred to as optical magnification) calculated on traditional microscope with eyepieces? Your typical lab microscope has a pair of eyepieces and some objective lenses installed, both of which have a specified magnification power. Microscopes usually come standard with a pair of 10x eyepiece, but can be swapped out for higher magnification pairs. Objective lenses, which are installed in a turret design, can have magnifications of 4x, 10x, 20x, or higher.

Magnification on a traditional microscope is calculated by multiplying the eyepiece magnification with the objective magnification that the turret is set to. A microscope with 10x eyepieces and 20x objective lenses will have 200x magnification during inspection. Since magnification is marked on the eyepieces and objective lenses, it is the easiest way to determine what specs you need for your application, as the magnification is calculated in the same way across all traditional microscopes with eyepieces and objective lenses.

Howto calculatemagnification of adrawing

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Video magnification is usually not as clearly defined on a digital microscope as optical magnification are on a traditional microscope with eyepieces and objective lenses. While some digital microscopes’ software have a magnification read out feature, this is not as common of a feature as you’d think. If you really wanted to find out the magnification your digital microscope is currently at, you would need to know the FOV first before taking the additional step to calculate the magnification on the microscope in relation to the monitor size. Instead of converting these values into magnification just to compare apples to apples, why not skip this extra step when you can figure out what digital microscope you need with just the field of view values?

Calculatingmagnification of a microscopeWorksheet

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In microscopy, microscopes with higher magnifications are known as high power microscopes, and lower magnification microscopes are low power. Microscopes are used in nearly every field with optical microscopes that can observe microorganisms in microbiology to electron microscopes that can probe individual molecules and particles.

Finding the magnification of each lens requires examining the casing of each lens. On the side of the casing is a series of numbers that includes a number followed by x, as 10x. This 10x shows that the lens magnifies an object to appear ten times larger than reality. Depending on the manufacturer, this magnification number may appear at the beginning or at the end of the number sequence. To calculate total magnification, find the magnification of both the eyepiece and the objective lenses. The common ocular magnifies ten times, marked as 10x. Standard microscope objective lenses magnify 4x, 10x and 40x. If the microscope has a fourth objective lens, the magnification will most likely be 100x. These figures represent the power of the objective lenses.

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