NDfilter chart

When searching for ND filters, we believe that labeling with the number of stops is the easiest way to understand exactly how they will affect your images. Our Kolari Pro ND Filters come in a wide range of strengths from 1-stop to 20-stops, and our Kolari Pro VND is available in 2-5 stops and 6-9 stops options.

Photobiomodulation of cells using near-infrared (NIR) monochromatic light can affect cell functions such as proliferation, viability, and metabolism in a range of cell types. Evidence for the effects of near-infrared light on endothelial cells has been reported, but the studies were mainly performed using VIS light emitted by low-energy lasers, because NIR wavelengths seemed negatively stimulate these cells. Cell viability, free radical-induced oxidative stress, NF-κB activation, nitric oxide release, mitochondrial respiration, and wound healing repair were assessed in human endothelial cells (HECV) irradiated with 808-nm diode laser light (laser setup = 1 W/cm2, 60 s, 60 J/cm2, CW vs measured energy parameter = 0.95 W/cm2, 60 s, 57 J/cm2, mode CW) emitted by an handpiece with flat-top profile. No difference in viability was detected between controls and HECV cells irradiated with 808-nm diode laser light for 60 s. Irradiated cells demonstrated higher proliferation rate and increased migration ability associated to moderate increase in ROS production without a significant increase in oxidative stress and oxidative stress-activated processes. Near-infrared light stimulated mitochondrial oxygen consumption and ATP synthesis in HECV cells. Short near-infrared irradiation did not affect viability of HECV cells, rather led to a stimulation of wound healing rate, likely sustained by ROS-mediated stimulation of mitochondrial activity. Our results demonstrating that near-infrared led to a shift from anaerobic to aerobic metabolism provide new insight into the possible molecular mechanisms by which photobiomodulation with 808-nm diode laser light protects against inflammation-induced endothelial dysfunction, seemingly promising to enhance their therapeutic properties.

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Where this gets tricky is knowing the mathematical order of operations for the different stops. For example, a 1-stop ND will reduce the light by 50% and a 5-stop will cut the light in half five times in a row. If you have a 1-second exposure without a filter and then put on a 1-stop ND filter, you have effectively halved the amount of light coming into your camera. In order to balance out the exposure, you’ll have to increase your shutter speed by doubling it. Now, your 1-second exposure becomes 2 seconds.

Since ND filters are used to reduce the amount of light in a scene, each stop is always halving the amount of light. The larger the stop number, the more light the filter blocks out.

Keywords: Human endothelial cells (HECV); Mitochondrial respiration; NIR-diode laser; Photobiomodulation (PBM); Wound regeneration.

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ND filters come in many different strengths. Depending on the manufacturer, these strengths may be notated in one of three ways: stops, optical density, or ND factor. Stops (sometimes referred to as exposure value) are fairly straightforward in that they tell you exactly how many stops your exposure will be adjusted by. Optical density is essentially 0.3 x the exposure value. This is the least common labeling system on the market. The ND factor is often listed as ND2, ND4, ND8, and so on. These numbers refer to the amount by which the light is reduced, ie. ND2 halves the light while ND4 reduces the light to one quarter. The chart below shows the translation between the three labeling systems.

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You can see how as the filter strength increases, the exposure time is doubled sequentially to compensate for the loss of light. When the light is cut in half, you need to double the shutter speed to maintain the same exposure. Add another ND stop, double the shutter speed again.