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General Advice: You should check to see if your microscope has a rack stop. If it does not have one, then be careful not to allow the objective lens to touch the slide as you may break the slide. When using a monocular microscope, the correct technique is to look through the eyepiece with one eye and keep the other eye open. Most new users, tend to close one eye. While many microscopists do close one eye, you will help avoid eye strain by keeping both eyes open. Finally, remember! When you view a specimen through a microscope, you are viewing an image through multiple lenses. As a result, the image is upside down and back-to-front so when you move the slide to the right, the image moves to the left and vice versa!Care & Maintenance of Your Microscope: Your compound microscope will last a lifetime if cared for properly and we recommend that you observe the following basic steps:

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Familiarization  First, familiarize yourself with all the parts of a microscope so that you can easily move from one part to another during operation. Moving and Placement: It is worth remembering that while a good quality microscope will last a lifetime, it is a sensitive scientific instrument that will suffer damage from sharp blows or impact. Always, therefore, carry your microscope in both hands. Grasp the arm with one hand and place the other hand under the base for support. Always place the microscope on a level and stable surface.Slide Preparation: Microscope slides should always be prepared with a cover slip or cover glass over the specimen. This will help protect the objective lenses if they touch the slide. To hold the slide on the stage fasten it with the stage clips. You can push down on the back end of the stage clip to open it.Focusing the Microscope:

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Figure 6a shows the maximum excitation wavelength for FITC at 495 nm, which results in the highest fluorophore absorption band (the dotted light blue curve).