Two-photon microscopy

Deep tissue two-photon microscopy

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Reynard Corporation offers single point diamond turning (SPDT) in-house capabilities to produce 6mm to 800mm diameter high-quality precision optics for the aerospace, military and commercial industries.

Multiphoton microscopy (also known as non-linear or two-photon microscopy) is an alternative to laser scanning (single photon) or deconvolution microscopy that provides distinct and clear advantages for three-dimensional imaging. Specifically, multiphoton excitation is superior for imaging living cells that reside within intact tissues such as brain slices, embryos, whole organs, and even entire animals. The technique provides optical sectioning with reduced absorption of excitation light in specimen regions removed from the objective focal plane, thus minimizing photobleaching and phototoxicity. The longer excitation wavelengths also enable increased depth penetration over confocal microscopy and are therefore more useful to deep imaging.

Our Nanoforms, Ultra Precision Machining Systems by Precitech are used for the diamond turning multi-stage process to achieve sub-nanometer level surface finishes and sub-micrometer form accuracies. Diamond Turning enables the machining of optics with complex geometries not achievable through other machining capabilities at an economical price.