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To achieve the full NA of 1.25, immersion oil is placed between the slide and the 100x objective. This prevents refraction and loss of resolution from the air-glass interface.

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Lower magnification gives a wide field of view to find and identify organisms. Medium magnifications like 400x are ideal for studying cell structure. 1000x is used for finer details like organelles.

It has a very high 1.25 numerical aperture and a short focal length of 0.2mm. At 100x the field of view is only 0.2mm wide.

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The objective lenses on most compound microscopes typically come in 4x, 10x, 40x, and 100x magnifications. Some microscopes may also have a 60x or 150x objective.

The field of view at 40x is 0.5mm across – much smaller than the lower power lenses but still enough to see most cell details. 40x is commonly used for studying cell structures.

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The 4x scanning objective has a numerical aperture of 0.10 and a focal length of 16mm. It has a field of view of 5mm across.

Start with the lowest power 4x or 10x objective to locate the specimen. Then increase magnification to observe specific structures. Use the 100x oil immersion lens for the finest details.

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The 10x objective lens typically has a numerical aperture of 0.25 and a focal length of 4mm. The field of view at 10x is around 2mm across.

The high NA paired with oil immersion allows the 100x objective to see fine subcellular details like organelles and chromosomes. It is used to study cellular structure and mitosis.

The Fresnel equations quantitatively show how unpolarized light becomes partially polarized after reflection from a dielectric surface--such as water or glass.

These lower magnification objectives provide a wide field of view which is useful for scanning slides and locating specimens. Resolution and fine detail are low, but the overview of the sample is clear.

Some microscopes may also have specialized oil immersion objectives like 60x or 150x. Like the 100x lens, these objectives require immersion oil to reach their maximum NA and magnifying power. Immersion oils are specially formulated to have specific refractive indexes matched to the lens.

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Plan apochromat objectives are high-quality optimized lenses that reduce optical aberrations and provide flat, sharp images. Fluorite lens elements allow a wider spectrum of light throughput. These advanced objectives paired with oil immersion provide the clearest high-magnification views.

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