Opticalresolution

https://www.mathworks.com/matlabcentral/answers/469971-point-spread-function-and-optical-transfer-function#answer_381717

Fluorescence images of this target can be analyzed using our web portal at FGS.QUELimaging.com. We are currently enrolling Beta testers for this site. A brief overview of the current features are available in the video below. The aim of this service is to provide system developers and researchers a reliable, repeatable and time-efficient method to analyze fluorescence images of these reference targets.

1951 USAFresolutiontest chart printable

https://www.mathworks.com/matlabcentral/answers/469971-point-spread-function-and-optical-transfer-function#answer_381717

https://www.mathworks.com/matlabcentral/answers/469971-point-spread-function-and-optical-transfer-function#answer_381723

ISO 12233 chart

USAF 1951 pdf

https://www.mathworks.com/matlabcentral/answers/469971-point-spread-function-and-optical-transfer-function#answer_381723

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This reference target is an ideal solution understand the resolution of your fluorescence imaging system. This target uses a negative USAF1951 chart with progressively smaller line-pairs and 1uM ICG-equivalent material to provide an estimate of the imaging system’s optimal resolution to determine the resolvable feature size in fluorescence mode. A contrast transfer function can be estimated by analyzing images of this target.

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