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Certain instruments are designed to accommodate additional high-power 60x or 100x objective lenses when extremely high magnification and resolution are critical, such as for cytology or microbiology applications.

The level of microscope magnification depends on the optical properties of both the ocular and objective lenses. The ocular lens magnifies the primary image 10x. The objectives provide progressively higher magnifying power of 4x, 10x, 40x, and sometimes 100x.

Objective lensmicroscope

Having a continuum of magnifications allows the microscope to accommodate samples of vastly different sizes from whole insect bodies down to single cells. A single high-power objective cannot cover this entire range.

The 40x or 100x high power objective produces the highest magnification and resolution to reveal subcellular structures and other intricate details not discernable with the lower powered lenses but has an extremely narrow field of view. It is used for critical inspection of key areas after initial surveys with lower-powered objectives.

Opticalmicroscope

Higher magnification requires higher resolution to realize the full benefit. The higher-powered objectives have correspondingly greater resolving power to take advantage of the increased magnification whereas the lower-power lenses have comparatively less resolution which is ample for their magnification level.

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The multiple objectives with parcentered optics allow users to quickly switch between lenses and magnifications to obtain just the right view. This facilitates efficient and intuitive workflows.

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The standard compound microscope contains 3 objective lenses with different powers, resolutions, and fields of view to provide a tiered viewing experience.

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The provision of 3 objective lenses with differing optical properties confers important complementary advantages that enhance the microscopy user experience and workflow efficiency.

Practically, low magnification facilitates efficient scanning of the overall specimen to find areas of interest to study further, saving significant time compared to searching blindly at high power. It provides necessary contextual orientation.

What is the purpose of the objective lens in a lightmicroscope

What does the objective lens do on amicroscope

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Confocal microscopy

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The 10x or 20x medium power objective delivers comfortable viewing magnification and reasonably high resolution to see some finer details in the context of the larger specimen structure. It is commonly used for routine examination, counting cells, measuring proportions, and making sketches.

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Some microscopes include extra low power 1x or 2x objectives for an even wider field of view to help orient the largest samples. These have become more common on inverted microscopes.

The compound light microscope is an indispensable tool used ubiquitously in science disciplines to visualize small objects in fine detail. Unlike simple magnifying glasses, the compound microscope uses two lens systems to enlarge specimens up to 1000x their actual size.