Objective lensmagnification

This super-corrected apochromat objective corrects a broad range of color aberrations to provide images that capture fluorescence in the proper location. Delivering a high degree of correction for lateral and axial chromatic aberration in 2D and 3D images, it offers reliability and accuracy for colocalization analysis.

The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

Different lenses are useful in different situations. Next time you are out in a forest or field, and come across an enormous mushroom, take a photo from 20cm distance using your 2 lenses, see the effect of the result, and the reason why someone might choose a close-up, wide-angle shot becomes self-evident.

For a longer explanation (and female models) check out JP's Slanted Lens video: "How Lens Focal Length Shapes the Face & Controls Perspective: A Lighting Tutorial".

This semi-apochromat objective series provides flat images and high transmission up to the near-infrared region of the spectrum. Acquiring sharp, clear images without color shift, they offer the desired quality and performance for fluorescence, brightfield, and Nomarksi DIC observations.

These semi-apochromat and achromat objectives are designed for integrated phase contrast observation of cell cultures. They are used in combination with a pre-centered phase contrast slider (CKX3-SLP), eliminating centering adjustments when changing the objective magnification.

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For relief contrast observation of living cells, including oocytes, in plastic vessels using transmitted light, these achromat objectives provide excellent field flatness.

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Here's an example from Outdoor Photographer, photography (c) by Rob Sheppard. Note how the features of the tree in the foreground are highlighted, but are also set in the context of the mountains in the background:

What does an objective lens do on a microscopegive

Doing a close-up allows you to use perspective to further exaggerate the relative sizes. The wide angle also allows you to include more context than a telephoto lens, especially at close range to the subject.

Optimized for multiphoton excitation imaging, these objectives achieve high-resolution 3D imaging through fluorescence detection at a focal point of a large field of view. They enable high-precision imaging of biological specimens to a depth of up to 8 mm for in vivo and transparent samples.

Designed for clinical research and routine examination work in the laboratory, these achromat objectives provide the level of field flatness required for fluorescence, darkfield, and brightfield observation in transmitted light.

High powerobjective microscopefunction

These semi-apochromat objectives enable phase contrast observation while providing a high level of resolution, contrast, and flatness for unstained specimens.

At short focal lengths you are looking around the object and obtaining an in-focus background and with long focal lengths you are looking at a smaller segment from a distance, flattening the object and blurring the background. Of course it's possible to make adjustments to distances to increase or reduce the effects and crop a higher resolution to simulate a closer shot.

Designed for low-magnification, macro fluorescence observation, this semi-apochromat objective offers a long working distance, a high NA, and high transmission of 340 nm wavelength light.

For relief contrast observation of living cells, including oocytes, in plastic vessels, our universal semi-apochromat objectives feature a long working distance. These also provide high image flatness and high transmission up to the near-infrared region.

These apochromat objectives are dedicated to Fura-2 imaging that features high transmission of 340 nm wavelength light, which works well for calcium imaging with Fura-2 fluorescent dye. They perform well for fluorescence imaging through UV excitation.

This article from Photography life 'focuses' mostly on landscape photography with wide angle lenses: https://photographylife.com/how-to-use-wide-angle-lenses

Optimized for polarized light microscopy, these semi-apochromat objectives provide flat images with high transmission up to the near-infrared region of the spectrum. They are designed to minimize internal strain to meet the requirements of polarization, Nomarski DIC, brightfield, and fluorescence applications.

Enabling tissue culture observation through bottles and dishes, these universal semi-apochromat objectives feature a long working distance and high contrast and resolution. Providing flat images and high transmission up to the NIR region, they are well suited for brightfield, DIC, and fluorescence observation.

Offering our highest numerical aperture values, these apochromat objectives are optimized for high-contrast TIRF and super resolution imaging. Achieve wide flatness with the UPLAPO-HR objectives’ high NA, enabling  real-time super resolution imaging of live cells and micro-organelles.

Digital Photo mentor also wrote an article about how to use wide angle lenses: https://www.digitalphotomentor.com/5-mistakes-beginners-make-using-a-wide-angle-lens-and-how-to-avoid-them/

For high-performance macro-observation, these apochromat objectives provide sharp, clear, flat images without color shift, achieving high transmission up to the near-infrared region of the spectrum. They perform well for fluorescence, brightfield, and Nomarksi DIC observations.

Types ofobjectivelenses

Dan Vojtěch's article: "Amazing how focal length affect shape of the face" demonstrates how changing your focal length and distance affects the appearance of everything. Pascal Vandecasteele uses the same photo in his article: "How focal length affect the shape of the face".

The reason I chose it is because it is very clear the creative possibilities of having a humungous first plane and a tiny far plane of the same person... Perspective.

Designed for clinical research and routine examination in labs using phase contrast illumination, these achromat objectives offer excellent field flatness.

Whatare the 3objectivelenseson a microscope

Jul 16, 2017 — Focal length is the distance measured in millimeters from the optical center (also known as the center nodal point) of the lens to the sensor ...

Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

Many microscopes have several objective lenses that you can rotate the nosepiece to view the specimen at varying magnification powers. Usually, you will find multiple objective lenses on a microscope, consisting of 1.25X to 150X.

Designed for phase contrast observation of cell cultures in transmitted light, these achromat objectives combine field flatness and easy focusing with cost efficiency. They are well suited for routine microscopy demands.

In the example below I took different photos of the same object, first twice with my Samyang 12 mm prime lense, then three times with my Sony E 18-200mm zoom lens, at various object and focal distances. I downsampled the images from 4000 to 1200 pixels wide but otherwise did not do any other post-processing than in-camera processing. The candle holder is about 9 cm high and 12 cm wide.

What doesthe stagedo on a microscope

Ken Rockwell has written a very interesting article about his: https://kenrockwell.com/tech/how-to-use-ultra-wide-lenses.htm

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To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

You would probably wish your lens could focus a bit closer than 20 cm if you want to make a picture like this: https://flic.kr/p/3Paa94

Depth of field (DOF), the area of apparent sharpness in an image, is one of the main creative controls in photography and videography.

These super apochromat objectives provide spherical and chromatic aberration compensation and high transmission from the visible to the near infrared. Using silicone oil or water immersion media, which have refractive indexes closely matching that of live cells, they achieve high-resolution imaging deep in living tissue.

"Wide angles create intimate relationships. Wide angles emphasize the photographer's height, distance, and relationship to the subject. When we look at a wide-angle portrait, the visual cues and exaggerated foreground-background perspectives tell us exactly where the photographer was, relative to the subject, within a broad environment.".

These extended apochromat objectives offer high NA, wide homogenous image flatness, 400 nm to 1000 nm chromatic aberration compensation, and the ability to observe phase contrast. Use them to observe transparent and colorless specimens such as live cells, biological tissues, and microorganisms.

"The shorter the focal length, the closer to the face. That is why the nose looks bigger in the 20mm lens shot than in the 200mm lens shot. If all photos were taken at the same camera distance, the face would look the same for each lens, however, the head size would change – smaller for the 20mm lens gradually getting larger up to the 200mm lens."

Microscope objectives come in a range of designs, including apochromat, semi-apochromat, and achromat, among others. Our expansive collection of microscope objectives suits a wide variety of life science applications and observation methods. Explore our selection below to find a microscope objective that meets your needs. You can also use our Objective Finder tool to compare options and locate the ideal microscope objective for your application.

These semi-apochromat long-working distance water-dipping objectives for electrophysiology deliver flat images for DIC and fluorescence imaging from the visible range to the near-infrared. Their high NA and low magnification enables bright, precise macro/micro fluorescence imaging for samples such as brain tissue.

Whatisobjective lensinmicroscope

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To me, the photos with the zoom lens appear more interesting for this purpose. The bokeh is stronger (even though all photos were taken at F/8.0) and the object fills more of the frame. What possibilities does an ultra-wide lens offer when I focus on an object that is close by?

Oct 1, 2024 — How to Calculate Gaussian Spot Size? · First, determine the wavelength (λ) of the laser. · Next, determine the focal length (f) of the lens used ...

I have a Samyang F/2.0 12 mm for the Sony E Mount, so the 35-mm equivalent focal length is 18 mm (crop sensor 1.5×). I use it mainly for landscapes and architecture in order to fit more in my frame than I can with my other lens, usually focussing close to infinity (I also use it for photography of starry nights such as the milky way or star trails, as it is also by far my fastest lens). I sometimes use it in small indoor spaces, with a focus perhaps down to 1–2 metre at the lowest. But I've never used the lower half of the focus ring, which includes focus down to 20 cm. When I want detail, I tend to use my zoomlens (18–200 mm). Under what circumstances would one use a wide angle lens with a focus less than (say) 50 cm?

For use without a coverslip or cover glass, these objectives prevent image deterioration even under high magnification, making them well suited for blood smear specimens. They also feature extended flatness and high chromatic aberration correction.

I have modified the animation offered on Dan and Pascal's pages to slow it down, and repeated the first and last frames at an even slower speed to demonstrate the difference:

For phase contrast observation of cell cultures, these universal semi-apochromat objectives provide long working distances and flat images with high transmission up to the near-infrared region. They help you achieve clear images of culture specimens regardless of the thickness and material of the vessel.

When a microscope objective shows a non-symmetric optical behavior, as in the case of spherical aberration (as well as astigmatism and/or coma), the wavefront ...

To get a close-up of something with a wide angle lens, you need to get extremely close. Everything seems about 3 times further away in the picture due to the focal length, so you need to get much closer than 50 cm to get something really close.

The compound microscope has two systems of lenses for greater magnification, 1) the ocular, or eyepiece lens that one looks into and 2) the objective lens, or ...

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... lenses of the patient`s eyeglasses with just water. It can be applied to the entire lens or to any region of the lens. SPECIFICATIONS. Range: 1.0 Diopter to ...

A very good way of using an ultra wide lens is to get really close and get something right in the viewers face. Using a wide angle lens to get everything in the frame often leads to very boring pictures because the viewer has no idea what the photo is about.

Microscopeparts

The distortion of wide angle lenses allows you to magnify the subject relative to the rest of the frame, leading to dramatic or otherwise interesting compositions.

As @Agent_L says in the comments, if you take photos with your 2 lenses at exactly the same short distance from something, the answer to your question becomes more obvious. If you are curious about this - and you must be, since you asked here - then you should simply take the photos and see for yourself.

Shoot a bride from the floor, shoot a sports person from the tip of the hand or feet, shoot an ant eating a dog, shoot some toys next to real people.

Unsure of what microscope objective is right for you? Use our guide on selecting the right microscope objective to weigh your options.

For clinical research requiring polarized light microscopy and pathology training, these achromat objectives enable transmitted polarized light observation at an affordable cost.

These extended apochromat objectives offers a high numerical aperture (NA), wide homogenous image flatness, and 400 nm to 1000 nm chromatic aberration compensation. They enable high-resolution, bright image capture for a range of applications, including brightfield, fluorescence, and confocal super resolution microscopy.

Probably the best comment comes from Em Jo Photo on DPReview's webpage: "Facial distortion of various focal lengths for headshots":