With our spectrometers, data can be collected and analyzed using our LabQuest 2, LabQuest 3, or free Vernier Spectral Analysis® software, which allows users to spend less time learning different interfaces and more time in the laboratory. Plus, Vernier Spectral Analysis works with Chrome™, iOS, Android™, Windows®, and macOS™ via Bluetooth® wireless technology or USB.

Save time and space with our suite of spectrometers. These innovative, easy-to-use instruments have a small footprint and collect data within seconds. In addition, they can be used in chemistry, biology, and physics classes at both the high school and college level.

Spectrometer lab labreport

Filter sets for fluorescence microscopy consist of excitation and emission bandpass filters and a dichroic beamsplitter. Filter sets play a critical role in that they direct excitation light from the light source to the sample and then separate it on the basis of wavelength, from fluorescence emitted from the specimen. Optimized filter sets are critical because fluorescence emission from a microscopic specimen is at least six orders of magnitude weaker than the excitation from the light source. Recognizing that optimized filter set specifications are critical for obtaining images with high signal: background contrast, Lumencor has developed uniquely high performance, multiband dichroic beamsplitters and multiband and single band emission filters for use with our AURA, SPECTRA, CELESTA, and ZIVA Light Engines.

Set consists of single band emitters 10-10871, 10-10872, 10-10873, 10-10874 and 10-10875. For detection of DAPI, FITC, TRITC, Cy5 and Cy7 and spectrally similar fluorophores in combination with pentaband dichroic 10-10858.

Single band Cy5 emitter, 691/64 nm, in mounting ring, 25.4 mm diameter. For detection of Cy5 and spectrally similar fluorophores

Spectrometer labprinciple

Single band DAPI emitter, 445/49 nm, in mounting ring, 25.4 mm diameter. For detection of DAPI, Hoechst 33342 and spectrally similar fluorophores

Single band YFP emitter, 547/20 nm, in mounting ring, 25.4 mm diameter. For detection of YFP and spectrally similar fluorophores.

Spectroscopylabhigh school

The Go Direct Visible Spectrophotometer is a robust, portable visible spectrophotometer. It uses a quality optical bench, which allows for high accuracy and reproducibility, making this visible spectrophotometer an ideal option for upper-level courses.

In the table below, specifications for filters and dichroics are given in the Description column. Download links give access to (x,y) transmission spectral data for each filter or dichroic.

The Vernier Flash Photolysis Spectrometer is a simple, user-friendly device for demonstrating the fundamental principles of chemical kinetics and photochemistry to undergraduate chemistry students. It measures the absorption and emission changes over time of a photoexcited sample at a single wavelength.

Single band mCherry emitter, 606/18 nm, in mounting ring, 25.4 mm diameter. For detection of mCherry, Alexa Fluor 594 and spectrally similar fluorophores

Single band TRITC emitter, 590/50 nm, in mounting ring, 25.4 mm diameter. For detection of TRITC, Cy3 and spectrally similar fluorophores

Single band DAPI emitter, 440/37 nm, in mounting ring, 25.4 mm diameter. For detection of DAPI and spectrally similar fluorophores.

Single band GFP emitter, 531/50 nm, in mounting ring, 25.4 mm diameter. For detection of FITC, GFP and spectrally similar fluorophores

Single band CFP emitter, 481/36 nm, in mounting ring, 25.4 mm diameter. For detection of CFP, BV480 and spectrally similar fluorophores

Single band mCherry emitter, 647/100 nm, in mounting ring, 25.4 mm diameter. For detection of mCherry, Alexa Fluor 594 and spectrally similar fluorophores.

Spectroscopy experiment physics

Pentaband dichroic optimized for fluorescence microscopy using excitation from from CELESTA Light Engine 405 nm, 477 nm, 545 nm, 639 nm and 748 nm laser lines. 45° incidence reflection of excitation and 0° transmission of fluorescence emission. Unmounted, 25 mm x 36 mm x 1 mm. For use in combination with pentaband emitter 10-10857.

Single band Cy7 emitter, 823/100 nm, in mounting ring, 25.4 mm diameter. For detection of Cy7 and spectrally similar fluorophores.

With the Go Direct Emissions Spectrometer, students can conduct emissions analysis; measure spectra of light-emitting samples; and examine emissions spectra of light bulbs, spectrum tubes, or the sun.

Single band FITC emitter, 510/31 nm, in mounting ring, 25.4 mm diameter. For detection of FITC, GFP and spectrally similar fluorophores

Spectroscopylabchemistry

Triple band emission filter optimized for fluorescence microscopy using excitation from 446 nm, 518 nm and 577 nm laser outputs of CELESTA Light Engines. 25 mm diameter in 3.5 mm mounting ring For detection of CFP, YFP, mCherry and spectrally similar fluorophores in combination with triple band dichroic 10-10835.

All emission filters and dichroics are supplied in unmounted form. Installation in filter cubes, filter wheels, or other instrumentation-specific mountings is required before use.

Spectroscopylabpdf

Pentaband emission filter optimized for fluorescence microscopy using excitation from CELESTA Light Engine 405 nm, 477 nm, 545 nm, 639 nm and 748 nm laser lines. 25 mm diameter in 3.5mm mounting ring. For detection of DAPI, FITC, TRITC, Cy5, Cy7 and spectrally similar fluorophores in combination with pentaband dichroic 10-10858.

Triple band emission filter optimized for spinning disk confocal microscopy using 446 nm, 518 nm and 577 nm laser excitation from ZIVA for Yokogawa Light Engine. 25 mm diameter in 3.5mm mounting ring. For detection of CFP, YFP, mCherry and spectrally similar fluorophores

Triple band emission filter optimized for fluorescence microscopy using excitation from BTY output channels of SPECTRA Light Engine. 25 mm diameter in 3.5 mm mounting ring. For detection of CFP, YFP, mCherry and spectrally similar fluorophores in combination with triple band dichroic 10-10837.

Triple band dichroic optimized for fluorescence microscopy using excitation from B and T output channels of CELESTA and ZIVA Light Engines. Unmounted, 25 mm x 36 mm x 1 mm. For use in combination with triple band emitter 10-10834.

What isspectrometerused for

Our multiband emitters and dichroics are optimized for compatibility with the electronically selectable excitation outputs of Lumencor’s colored-selective Light Engines. These systems enable fast multicolor imaging without the need for filter wheels or other positioning devices to execute filter interchanges. Single bandpass filters are offered for use in situations where fluorescence crosstalk (e.g. detection of FITC fluorescence derived from violet (DAPI) excitation) confounds the identification of fluorescently labeled components of the specimen.

Pentaband dichroic optimized for spinning disk confocal microscopy using 405 nm, 488 nm, 577 nm, 639 nm and 748 nm laser excitation from ZIVA for Yokogawa CSU-W1 Light Engine. 0° transmission of excitation and 45° incidence reflection of fluorescence emission. Unmounted, 22.2 mm x 25.0 mm x 1.0 mm.

Pentaband emission filter optimized for fluorescence microscopy using excitation from VCGRnIR output channels of SPECTRA Light Engine. 25 mm diameter in 3.5 mm mounting ring. For detection of DAPI, FITC, TRITC, Cy5, Cy7 and spectrally similar fluorophores in combination with pentaband dichroic 10-10860.

Triple band dichroic optimized for fluorescence microscopy using excitation from BTY output channels SPECTRA Light Engines. Unmounted, 25 mm x 36 mm x 1 mm. For use in combination with triple-band emitter 10-10836.

With Vernier Spectral Analysis, students can collect a full spectrum and explore topics such as Beer’s law, enzyme kinetics, plant pigments, and Planck’s constant.

The Go Direct UV-VIS Spectrophotometer generates full spectra, Beer’s law data, and kinetic traces of ultraviolet and visible-absorbing samples (such as aspirin, DNA, proteins, and NADH).

Transmission spectra of CELESTA VCGRN pentaband dichroic (10-10858) and emitter (10-10857) superimposed on the CELESTA Light Engine violet, cyan, green, red, and near-infrared output lines.

Pentaband dichroic optimized for fluorescence microscopy using excitation from VCGRnIR output channels of SPECTRA Light Engine. Unmounted, 25 mm x 36 mm x 1 mm. For use in combination with pentaband emitter 10-10859.

Spectrometer labreport

The Go Direct SpectroVis Plus Spectrophotometer is an affordable way to introduce your students to spectroscopy. Its battery power and compact size make it a great option for field applications.

Pentaband emission filter optimized for spinning disk confocal microscopy using 405 nm, 488 nm, 577 nm, 639 nm and 748 nm laser excitation from ZIVA for Yokogawa Light Engine. 25 mm diameter in 3.5mm mounting ring. For detection of DAPI, FITC, mCherry, Cy5, Cy7 and spectrally similar fluorophores.

The Go Direct Fluorescence/UV-VIS Spectrophotometer measures fluorescence and absorbance spectra of a diverse array of samples, such as quinine sulfate, fluorescein, chlorophyll, and fluorescent proteins.

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Triple band dichroic optimized for spinning disk confocal microscopy using 446 nm, 518 nm and 577 nm laser excitation from ZIVA for Yokogawa CSU-W1 Light Engine. 0° transmission of excitation and 45° incidence reflection of fluorescence emission. Unmounted, 22.2 mm x 25.0 mm x 1.0 mm.