light sheet microscopy中文

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Spectralon. Spectralon is a highly Lambertian material or coating that has the highest diffuse reflectance of any known material over the UV-VIS-NIR region ...

LSFM2024

The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly isotropic optical sections. However, increased axial resolution, via pinhole optics, comes at the cost of both speed and excitation efficiency. Light sheet fluorescent microscopy (LSFM), a century-old idea made possible with modern developments in both excitation and detection optics, provides sub-cellular resolution and optical sectioning capabilities without compromising speed or excitation efficiency. Over the past decade, several variations of LSFM have been implemented each with its own benefits and deficiencies. Here we discuss LSFMfundamentals and outline the basic principles of several major light-sheet-based imaging modalities (SPIM, inverted SPIM, multi-viewSPIM, Bessel beam SPIM, and stimulated emission depletion SPIM) while considering their biological relevance in terms of intrusiveness, temporal resolution, and sample requirements.

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ZEISS Lightsheet

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2024114 — The meaning of OPTIC is of or relating to vision or the eye. How to use optic in a sentence.

Nov 13, 2015 — The Molecular Expressions microscopy primer reviews basic and advanced topics and concepts in optics, light, color, optical microscopy, ...

Dec 2, 2017 — ... formula : angle = lambda / (2 * pi ... Airy disk size and Uncertainty principle ... It can't be used to estimate airy disk diameter, but it can tell ...

LSFM

If your lenses are in good shape and scratch-free, we can add the AR coating and send them back to you, good as new. The AR coating will reduce glare, improve ...

Light sheet fluorescence microscopy

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LSFMmeaning

LsfmLE SSERAFIM

N2 - The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly isotropic optical sections. However, increased axial resolution, via pinhole optics, comes at the cost of both speed and excitation efficiency. Light sheet fluorescent microscopy (LSFM), a century-old idea made possible with modern developments in both excitation and detection optics, provides sub-cellular resolution and optical sectioning capabilities without compromising speed or excitation efficiency. Over the past decade, several variations of LSFM have been implemented each with its own benefits and deficiencies. Here we discuss LSFMfundamentals and outline the basic principles of several major light-sheet-based imaging modalities (SPIM, inverted SPIM, multi-viewSPIM, Bessel beam SPIM, and stimulated emission depletion SPIM) while considering their biological relevance in terms of intrusiveness, temporal resolution, and sample requirements.

AB - The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly isotropic optical sections. However, increased axial resolution, via pinhole optics, comes at the cost of both speed and excitation efficiency. Light sheet fluorescent microscopy (LSFM), a century-old idea made possible with modern developments in both excitation and detection optics, provides sub-cellular resolution and optical sectioning capabilities without compromising speed or excitation efficiency. Over the past decade, several variations of LSFM have been implemented each with its own benefits and deficiencies. Here we discuss LSFMfundamentals and outline the basic principles of several major light-sheet-based imaging modalities (SPIM, inverted SPIM, multi-viewSPIM, Bessel beam SPIM, and stimulated emission depletion SPIM) while considering their biological relevance in terms of intrusiveness, temporal resolution, and sample requirements.

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CS505MUP -Ask a technical question Ask a technical question. Package Weight: 2.03 lbs / EACH. Discontinued: Dec 16 ...

Nov 15, 2024 — It also boasts a minimum focusing distance of 12 inches (30 centimeters). A Nikon Z fc camera with a vintage design is shown from the front ...

by V Červeny · 1983 · Cited by 155 — As a limiting case of infinitely broad Gaussian beams, the paraxial ray approximation is obtained. The properties and possible applications of Gaussian beams ...

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