Optical 101: Vision - optical 101

 2024-07-15 00:02:34

To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

Types ofobjectivelenses

MXPLFLN objectives add depth to the MPLFLN series for epi-illumination imaging by offering a simultaneously improved numerical aperture and working distance.

High powerobjectivelens

The IMX462 sensor is the newest "6th Generation" sensor from Sony. With each successive generation, Sony has made significant improvements in sensor sensitivity. In the 6th generation of sensors, back-illuminated technology is enhanced with physically deeper photo sites allowing the capture of longer wavelength photons in the red and near infrared portion of the spectrum. The back-illuminated structure of the pixel also allows more photons, include these longer wavelength photons, to be captures in less time. This greatly improves the sensitivity of the pixel to near infrared in the 800 - 1000nm range which is approximately doubled when compared to cameras using the back-illuminated 290 sensor.

Many microscopes have several objective lenses that you can rotate to view the specimen at varying magnification powers. Usually, you will find multiple objective lenes on a microscope, consisting of 1.25X to 150X.

Objectivelens function

Objectivelens telescope

The QHY5III462C pixel size and array is essentially the same as the popular QHY5III290 camera but the new QHY5III462 model offers two very significant improvements over its predecessor: Extended NIR QE (with NIR monochrome imaging capability) and sHCG or Super High Conversion Gain.

The QHY5III462C comes with two free 1.25-inch filters. The IR850 filter is a long wavelength pass filter that allows wavelengths > 850nm to pass through. The UV/IR filter passes visible light and blocks the NIR. With these two filters you can capture both RGB color images and NIR monochrome images with the same camera. At NIR wavelengths, the RGB filters become transparent and the camera behaves like a monochrome IR camera.

MicrometerThis product may not be available in your area.View ProductMPLAPON Our MPLAPON plan apochromat objective lens series provides our highest level of chromatic correction and resolution capability, along with a high level of wavefront aberration correction. View ProductMPLAPON-Oil Our MPLAPON-Oil objective is a plan apochromat and oil immersion lens that provides our highest level of chromatic correction and resolution capability. The numerical aperture of 1.45 offers outstanding image resolution. View ProductMXPLFLN MXPLFLN objectives add depth to the MPLFLN series for epi-illumination imaging by offering a simultaneously improved numerical aperture and working distance. View ProductMXPLFLN-BD MXPLFLN-BD objective lenses add depth to the MPLFLN series for epi-illumination imaging by offering simultaneously improved numerical aperture and working distance. View ProductMPLN Our MPLN plan achromat lens series is dedicated to brightfield observation and provides excellent contrast and optimal flatness throughout the field of view. View ProductMPLN-BD Our MPLN plan achromat lens series is designed for both brightfield and darkfield observation and provides excellent contrast and optimal flatness throughout the field of view. View ProductMPLFLN The MPLFLN objective lens has well-balanced performance with a semi-apochromat color correction, a fair working distance, and a high numerical aperture. It is suitable for a wide range of applications. View ProductMPLFLN-BD The MPLFLN-BD objective lens has semi-apochromat color correction and suits a wide range of industrial inspection applications. It is specially designed for darkfield observation and examining scratches or etchings on polished surfaces. View ProductLMPLFLN Our LMPLFLN lens is part of our plan semi-apochromat series, providing longer working distances for added sample safety and observation with increased contrast. View ProductLMPLFLN-BD Our LMPLFLN-BD brightfield/darkfield objective lens is part of our plan semi-apochromat series, providing longer working distances for added sample safety and observation with increased contrast. View ProductSLMPLN The SLMPLN plan achromat objective lens offers an exceptionally long working distance and the image clarity that you expect from the Olympus UIS2 optical system. It is ideal for electronic assembly inspection and other similar applications. View ProductLCPLFLN-LCD The LCPLFLN-LCD objective lenses are optimal for observing samples through glass substrates, such as LCD panels. The adoption of optical correction rings enables aberration correction according to glass thickness. View ProductLMPLN-IR/LCPLN-IR Our LMPLN-IR and LCPLN-IR plan achromat lenses have a long working distance and are specifically designed for optimal transmission in the near-infrared region (700–1300 nm wavelengths). View ProductWhite Light Interferometry Objective Lens This objective lens is designed for the Mirau style of white light interferometers and maintains a high level of temperature tolerance. The optimized numerical aperture of 0.8 provides improved light gathering, with a working distance of 0.7 mm. View Product

The QHY5III462C has "Super High Conversion Gain" capability. By using a lower capacitance, a small amount of charge can be converted to a high voltage resulting in higher sensitivity in low-light conditions. The readout noise of the QHY5III462C is as low as 0.5e-. The following pictures demonstrate the low light improvement over the 290 sensor. The QHY5III462C image is on the left and the QHY5III290C image on the right. The low light conditions and exposures are identical for each image. This image shows the QHY5III462C has higher sensitivity and SNR than the QHY5III290C in the same conditions.cameras:

Objectivelens microscope

Olympus microscope objective lenses for industrial inspections offer outstanding optical performance from the visible light to near-infrared region. At Evident, we offer an extensive selection of Olympus objectives suited to specific inspection requirements and tasks. Our MXPLFLN-BD objective is designed for darkfield observation and examining scratches on polished surfaces, while our SLMPLN objective is ideal for electronic assembly inspection. Find your ideal microscope objective today for your inspection task. No matter your requirements, Olympus objective lenses have you covered.

MXPLFLN-BD objective lenses add depth to the MPLFLN series for epi-illumination imaging by offering simultaneously improved numerical aperture and working distance.

Objectivelens microscope function

The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

Objectivelens magnification

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Sony's new sHCG (Super High Conversion Gain) technology produces a higher voltage from fewer photons resulting in greater sensitivity even at very low levels of light. With sHCG technology, even in visible light, the QHY5III462C has higher sensitivity than the back-illuminated IMX290 based cameras.

To produce the highest detail with the greatest dynamic range, many planetary imagers prefer to capture hundreds or thousands of images in as short a time as possible and then process the results into one final combined image. This requires high sensitivity for short exposures and low read noise for combining multiple images. The QHY5III462C will output 135 frames per second at full resolution. With its exceptionally high QE and extraordinarily low (0.5e-) read noise, it is the perfect camera for planetary imaging.

Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

The QHY5III462C uses Sony's latest generation IMX462 back-illuminated sensor with extended red and near infrared response. It has nearly double the red and NIR sensitivity as previous generation BSI sensors and even greater sensitivity compared to front illuminated sensors. Because the RGB filters become transparent at NIR wavelengths, the camera captures RGB color images in visible light and monochrome images in NIR.

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