Because of the complex surface profiles, the fabrication of aspherical lenses are more difficult as compared with conventional sphere lenses. Several methods can be used to produce aspheric lenses. Each of these methods has its own unique advantages and limitations.

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The depth of focus is greatest on the lowest power objective. Each time you switch to a higher power, the depth of focus is reduced. Therefore a smaller part of the specimen is in focus at higher power. Again, this makes it easier to find an object on low power, and then switch to higher power after it is in focus. A common exercise to demonstrate depth of focus involves laying three different colored threads one on top of the other. As the observer focuses down, first the top thread comes into focus, then the middle one, and finally the bottom one. On higer power objectives one may go out of focus as another comes into focus.

Microscopeparts

Microinjection molding or polymer molding method has been used to produce hybrid precision glass-polymer lenses by adding a fairly thin layer of aspherized optical polymer to a conventional glass lens such as achromatic lens. The molding happens at room temperature instead of at a high temperature, there is less stress induced in the mold. The tooling costs are low because of the easy fabrication of the molds. Glass-polymer hybrids are widely used in optical systems because of the light weight and low cost for the production part. The polymer is also not as durable as glass, making this is an unideal solution for surfaces that will be exposed to harsh environments.

In addition to molded glass lenses, molded plastic lenses are also available. Plastic injection molding is injecting the molten plastic into the aspheric mode for producing plastic aspheric lenses. Compared with glass, plastic does not have good thermal stability and scratch resistance. While this method cannot produce high precision aspheric lenses, it does allow for mass production at extremely low cost following relatively high up-front tooling charges. Plastic lenses are describes as low cost, low weight and ease of adding mounting features. Plastic aspheres are widely used in optical systems that require moderate quality and low thermal stability.

Types ofobjective lenses

For optical engineers, it is important to understand the manufacturing techniques and choose the best manufacturing method with considering the lens application, performance requirements, development cost, sample cost, production part cost, and project timeline.

High powerobjective microscopefunction

The compound microscope is a useful tool for magnifying objects up to as much as 1000 times their normal size. Using the microscope takes lots of practice. Follow the procedures below both to get the best results and to avoid damaging the equipment.

The most notable advantage of using an aspherical lens is the reduction in spherical aberration commonly seen in spherical lenses. Spherical aberration occurs when a lens is not capable of focusing all the incident light rays on the exact same point. Any spherical optical surface, even a perfectly designed and manufactured one, will still exhibit spherical aberration. This inherent defect of a spherical surface causes incident light rays to focus at different points and create a blur.  By adjusting the surface constants and aspheric coefficients, the surface of an asphere can be designed to eliminate spherical aberration. Aspheres provide true diffraction-limited spot sizes and the lowest wavefront error. Figure 2 shows a spherical lens in a focusing system with significant spherical aberration when compared to an aspheric lens.

Whatis the magnification of the ocular lens

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*** Don't hoard slides! You can only view one at a time, so that's all you should be holding. Return it before getting another, and if you break it, tell your instructor so that it can be properly cleaned up and replaced! ***

Many factors such as wavelength requirements of the application and manufacturing cost will determined the material selection.  Below is a summary of materials that can be use with each manufacturing process.

Objectivelens function

Whatare the 3objective lenses on a microscope

Figure 1 shows the surface profile comparison of an aspherical lens a spherical lens. Unlike the spherical lens with a constant radius of curvature, the biggest feature of aspheric lenses is that the radius of curvature continuously changes with the radial distance from its optical axis. An aspheric lens can be designed to minimize aberrations or improve performance of optical systems by adjusting the conic constant and aspheric coefficients of the curved surface of the lens.

A spherical surface is defined by a single radius of curvature. Aspherical lenses have at least one surface that is not spherical. Optical Engineers typically consider the aspheres to be non-spherical, rotational symmetric surfaces. The radius of curvature of an asphere gradually changes from the center of the lens to the edge.

Whatisobjectivelens inmicroscope

Similar to grinding and polishing, single point diamond turning (SPDT) can be used to manufacture single lenses one at a time. Diamond turning offers a high level of precision in the quality of the optical surfaces but is somewhat limited in choice of materials. However, the tool size used in SPDT is significantly smaller than in precision polishing, producing surfaces with improved surface finishes and form accuracies. Standard optical glasses cannot be shaped through diamond turning, whereas plastics, metal, and crystals can.

Due to the advantages in improving the optical performance and/or reducing the number of lens elements of optical systems, aspheres are increasingly being used in optical systems for all kinds of applications including laser focusing/collimating, LED illumination, optical metrology, optical imaging, digital cameras, CD players, high-end microscopy instruments, etc.

Whatdoes the stagedo on a microscope

Comparing with molded plastics lenses, molded glass lenses have greater consistency in performance from lens to lens due to the thermal stability, high optical quality and greater durability of glass.

For decades, the method of grinding and polishing one aspheric lens at a time has been the standard manufacturing process. Most recently, computer numerically controlled (CNC) precision polishing can automatically adjust tool dwell parameters to form the aspheric shape accurately. When higher quality polishing is required, magneto-rheological finishing (MRF) can be adopted because of the high removal rate to correct the surface profile. While other manufacturing techniques require high-cost molds, precision polishing method utilizes standard tooling, which make it be the first choice for prototyping and low volume production.

Precision glass molding is a manufacturing technique of heating the glass material in a high temperature until it becomes malleable enough to be pressed uniformly into an aspheric mold. After the glass and molds gradually cools down to the room temperature, the resulting lenses maintain the shape of the mold. The initial cost of creating precision glass molds is high. However, once the mold is finished the incremental cost for each lens is lower than that of standard manufacturing techniques. This manufacturing technique is especially suitable for high volume production of high quality aspherical lenses.

When drawing what you see under the microscope, follow the format shown below. It is important to include a figure label and a subject title above the image. The species name (and common name if there is one) and the magnification at which you were viewing the object should be written below the image. All relevant parts of the drawing should be labelled on the right side of the image using straight lines. Lines should not cross. Drawings should be done in pencil, while labels should be in pen or typed. Remember that total magnification is determined by multiplying the ocular x objective.

Aspherical lenses with a complex surface profile can be designed to reduce or minimize spherical aberration and also other aberrations such as astigmatism, field curvature, and distortion. In optical systems, multiple spherical elements can be replaced by a single asphere without a loss in performance. In many cases, using aspherical lenses can significantly improve the system performance and also reduce the system weight, size, and manufacture cost.

The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power. Therefore, it is best to find an object on low power, center it, and then switch to the next higher power and repeat.