Multi-Photon Imaging - multiphoton excitation microscopy
The compound microscope is a useful tool for magnifying objects up to as much as 1000 times their normal size. Using the microscope takes lots of practice. Follow the procedures below both to get the best results and to avoid damaging the equipment.
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*** Don't hoard slides! You can only view one at a time, so that's all you should be holding. Return it before getting another, and if you break it, tell your instructor so that it can be properly cleaned up and replaced! ***
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The main difference between a simple microscope and a compound microscope lies in their optical systems and magnification capabilities. Optical System. Simple ...
Ultrafast fiber lasers are also increasingly being used in new and emerging applications such as biomedical microscopy and imaging, spectroscopy and for advanced scientific research.
Ultrashort pulse amplification is challenging because even low-energy pulses exhibit sufficient peak power to damage the amplifier. Stretching the pulse over time reduces its peak power and allows for optimal amplification without distortion—further stretching enables higher amplification. Recompressing the pulse following amplification returns it to its original ultrashort duration with significantly increased peak power—often in the gigawatt range. TeraXion’s line of ultrafast laser components maximizes both performance and cost effectiveness. These products include fixed and tunable pulse stretchers as well as stretcher+compressor matched pairs.
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When drawing what you see under the microscope, follow the format shown below. It is important to include a figure label and a subject title above the image. The species name (and common name if there is one) and the magnification at which you were viewing the object should be written below the image. All relevant parts of the drawing should be labelled on the right side of the image using straight lines. Lines should not cross. Drawings should be done in pencil, while labels should be in pen or typed. Remember that total magnification is determined by multiplying the ocular x objective.
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The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power. Therefore, it is best to find an object on low power, center it, and then switch to the next higher power and repeat.
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Multiphoton microscopy (MPM) is a powerful imaging technique widely used in biological and biomedical research. Unlike traditional fluorescence microscopy, which uses single-photon excitation, MPM employs multiple photons to excite a fluorescent molecule. One of the biggest challenges to the widespread adoption of multiphoton microscopy is finding a suitable light source for efficient fluorophore excitation. To reach the high peak power needed for nonlinear optical processes, femtosecond mode-locked lasers are used.
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Early on, TeraXion recognized the emerging importance of ultrafast lasers also known as ultrashort pulse lasers for industrial and medical applications. To address this need, TeraXion has developed a line of high-quality pulse stretchers and dispersion management reflectors that are robust, cost-effective, and versatile.
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Ultrafast lasers are crucial for precise material processing in electronics, automotive parts, and medical components, offering high peak power and precise cold ablation machining capabilities. Their design prioritizes quality components to ensure cost-effectiveness and robust performance in 24/7 manufacturing environments. Chirped-pulse amplification boosts pulse energies to meet advanced manufacturing needs beyond what mode-locked oscillators alone can provide.
Most industrial pico- and femtosecond laser systems utilize mode-locking to generate ultrashort pulses. The challenge with mode-locking is that the process requires a broad frequency band, but all the different wavelengths must maintain a defined phase relation as the pulse propagates within the cavity. TeraXion’s all-fiber dispersion-management reflectors provide precise control over the chromatic dispersion within the laser cavity, ensuring stable and short pulses.
Terahertz radiation possesses unique properties, such as the ability to penetrate various non-conductive materials like plastics, fabrics, and biological tissues, without causing ionization. The success of deploying THz technologies heavily depends on the availability of compact, efficient, and affordable THz sources. Femtosecond lasers are crucial tools for generating terahertz (THz) radiation due to their ability to produce extremely short, high-intensity pulses.
Due to the attenuation and distortion characteristics of POF, it is much lower in performance than glass fiber. Until now, POF is rarely used.
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The depth of focus is greatest on the lowest power objective. Each time you switch to a higher power, the depth of focus is reduced. Therefore a smaller part of the specimen is in focus at higher power. Again, this makes it easier to find an object on low power, and then switch to higher power after it is in focus. A common exercise to demonstrate depth of focus involves laying three different colored threads one on top of the other. As the observer focuses down, first the top thread comes into focus, then the middle one, and finally the bottom one. On higer power objectives one may go out of focus as another comes into focus.