The most common principle of optical imaging is that with a single lens, or similarly with a multiple-lens system, also called an objective. This is explained ...

Uv cutoffchemistry

$^\textit{a}$ $\mathrm{pH}$ range allowed with this buffer (conservative estimate). $^\textit{b}$ Absorbance $<0.5\mathrm{A};$ from Ref. 7. $^\textit{c}$ Requires addition of an acid (e.g., acetic or phosphoric). $^\textit{d}$ tris(hydroxymethyl)aminomethane. $^\textit{e}$ 1,3-bis[Tris(hydroxymethyl)methylamino]propane.

AcnUV cutoff

Zero Aperture Series Iris Diaphragms used for high quality movements and total light extinction are available at Edmund Optics.

Snyder cites an article by Li [1] as the primary source for UV cutoff values ("Ref 7" below) for various buffers [2, p. 299]:

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UV cutoffof water

When I want to check a $\mathrm{p}K_\mathrm{a}$ value I look it up in CRC Handbook. Is there such source for UV cut-off values of various buffers?

UV cutoffof methanol

$$\begin{array}{lrcr} \hline \text{Buffer} & \mathrm{p}K_\mathrm{a} & \text{Buffer Range}^\textit{a} & \text{UV Cutoff}^\textit{b} \\ \hline \text{Trifluoracetic acid} & \gg 2 & 1.5{–}2.5 & \pu{210 nm}~(0.1\,\%) \\ \text{Phosphoric acid/mono- or di-K} & 2.1 & <3.1 & <\pu{200 nm}~(0.1\,\%) \\ \quad\text{phosphate} & 7.2 & 6.2{–}8.2 & \\ & 12.3 & 11.3{–}13.3 & <\pu{200 nm}~(\pu{10 mM}) \\ \text{Citric acid/tri-K citrate} & 3.1 & & \\ & 4.7 & 2.1{–}6.4 & \pu{230 nm}~(\pu{10 mM}) \\ & 5.4 & & \\ \text{Formic acid/K-formate} & 3.8 & 2.8{–}4.8 & \pu{210 nm}~(\pu{10 mM}) \\ \text{Acetic acid/K-acetate} & 4.8 & 3.8{–}5.8 & \pu{210 nm}~(\pu{10 mM}) \\ \text{Mono-/di-K carbonate} & 6.4 & 5.4{–}7.4^\textit{c} & <\pu{200 nm}~(\pu{10 mM}) \\ & 10.3 & 9.3{–}11.3 & <\pu{200 nm}~(\pu{10 mM}) \\ \text{Bis-tris propane}^\textit{e}\cdot\ce{HCl}/\text{Bis-tris} & 6.8 & 5.8{–}7.8 & \pu{215 nm}~(\pu{10 mM}) \\ \quad\text{propane} & 9.0 & 8.0{–}10.0 & \pu{225 nm}~(\pu{10 mM}) \\ \text{Tris}^\textit{d}\cdot\ce{HCl}/\text{tris} & 8.3 & 7.3{–}9.3 & \pu{205 nm}~(\pu{10 mM}) \\ \text{Ammonium chloride/ammonia} & 9.2 & 8.2{–}10.2 & \pu{200 nm}~(\pu{10 mM}) \\ \text{1-Methylpiperidine}\cdot\ce{HCl}/\text{1-} & & & \\ \quad\text{Methylpiperidine} & 10.1 & 9.1{–}11.1 & \pu{215 nm}~(\pu{10 mM}) \\ \text{Triethylamine}\cdot\ce{HCl}/\text{triethylamine} & 11.0 & 10.0{–}12.0 & <\pu{200 nm}~(\pu{10 mM}) \\ \hline \end{array} $$

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UV cutoffof solvents

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Acetic acidUV cutoff

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