Assuming that \(d_{ik}\) is the 16 bit disparity value at column \(i\) and row \(k\) of a disparity image with \(w\) columns and \(h\) rows, the 3D reconstruction in meters can be written with the GenICam parameters as

GenICamPython

Decker, E. E., Maitra, U.: Conversion of γ-hydroxyglutamate to glyoxylate and alanine; purification and properties of the enzyme system. J. biol. Chem. 237, 2218–2227 (1962).

Previously, it was deduced from inhibitor experiments that isocitrate lyase (EC 4.1.3.1.) is synthesized de novo in watermelon cotyledons during the first 3 days of germination, which explains the sharp increase of activity during this period. The following decrease of activity was interpreted as the result of a limited half life of the enzyme molecule (Hock and Beevers, 1966).

The error image contains 8 bit unsigned integer values. The error \(e_{ik}\) must be multiplied by the scale value given in the GenICam feature Scan3dCoordinateScale to get the disparity-error values \(d_{eps}\) in pixels. According to the description in Confidence and error images, the depth error \(z_{eps}\) in meters can be computed with GenICam parameters as

GenICamCamera

The disparity image contains 16 bit unsigned integer values. These values must be multiplied by the scale value given in the GenICam feature Scan3dCoordinateScale to get the disparity values \(d\) in pixels. To compute the 3D object coordinates from the disparity values, the focal length and the baseline are required. These parameters are transmitted as GenICam features FocalLengthFactor and Baseline. The FocalLengthFactor value has to be multiplied by the width of the disparity image to get the focal length \(f\) in pixels for the given disparity image resolution. Knowing these values, the pixel coordinates and the disparities can be transformed into 3D object coordinates in the sensor coordinate frame using the equations described in Computing depth images and point clouds.

Gientka-Rychter, A., Cherry, J. H.: De novo synthesis of isocitritase in peanut (Arachis hypogaea L.) cotyledons. Plant Physiol. 43, 653–659 (1968).

Genicamapp

Filner, P., Varner, J. E.: A test for de novo synthesis of enzymes: density labeling with H2 18O of barley α-amylase induced by gibberellic acid. Proc. nat. Acad. Sci. (Wash.) 58, 1520–1526 (1967).

GenICamdownload

This hypothesis has been confirmed now by density labeling experiments of isocitrate lyase with deuterium. Seedlings grown from day 0 on D2O (80 vol. %) contained a heavier enzyme at the time of maximum activity than control seedlings grown on H2O (Fig. 6). No incorporation of deuterium into isocitrate lyase, however, was detectable when the cotyledons were labeled only from day 3 1/2 on, i.e. after the stage of maximum activity had been passed (Fig. 10), in spite of the fact that D2O was taken up from the cotyledons in considerable quantities. —These results prove at the same time that density labeling of the isocitrate lyase during early stages of germination was a result of de novo synthesis rather than a mere artifact produced by isotopic exchange.

Breidenbach, R. W., Beevers, H.: Association of the glyoxylate cycle enzymes in a novel subcellular particle from Castor bean endosperm. Biochem. biophys. Res. Commun. 27, 462–469 (1967).

The following list gives an overview of the relevant GenICam features of the rc_visard that can be read and/or changed via the GenICam interface. In addition to the standard parameters, which are defined in the Standard Feature Naming Convention (SFNC, see http://www.emva.org/standards-technology/genicam/genicam-downloads/), rc_visard devices also offer custom parameters that account for special features of the Stereo camera and the Stereo matching component.

Each image comes with a buffer timestamp and the PixelFormat given in the above table. This PixelFormat should be used to distinguish between the different image types. Images belonging to the same acquisition timestamp can be found by comparing the GenICam buffer timestamps.

GenICamsoftware

GigE Vision® uses GenICam to describe the camera/device features. For more information about this Generic Interface for Cameras see http://www.genicam.org/.

GenICaminterface

GenICamC++

Roboception provides tools and a C++ API with examples for discovery, configuration, and image streaming via the GigE Vision/GenICam interface. See http://www.roboception.com/download.

The rc_visard supports jumbo frames of up to 9000 bytes. Setting an MTU of 9000 on your GigE Vision client side is recommended for best performance.

Davis, B. J.: Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–427 (1964).

Longo, C. P.: Evidence for de novo synthesis of isocitratase and malate synthetase in germinating peanut cotyledons. Plant Physiol. 43, 660–664 (1968).

Gigabit Ethernet for Machine Vision (“GigE Vision®” for short) is an industrial camera interface standard based on UDP/IP (see http://www.gigevision.com). The rc_visard is a GigE Vision® version 2.0 device and is hence compatible with all GigE Vision® 2.0 compliant frameworks and libraries.

Hock, B. Die zeitliche Dauer der Isocitrat-Lyase-Synthese in Kotyledonen von Wassermelonenkeimlingen. Planta 93, 26–38 (1970). https://doi.org/10.1007/BF00387649

GenICamBrowser

Hu, A. S. L., Bock, R. M., Halvorson, H. D.: Separation of labeled from unlabeled proteins by equilibrium density gradient sedimentation. Anal. Biochem. 4, 489–504 (1962).

Gerhardt, B.-P., Beevers, H.: Developmental studies on glyoxysomes in Ricinus endosperm. J. Cell Biol. 44, 94–102 (1970).

The confidence image contains 8 bit unsigned integer values. These values have to be divided by 255 to get the confidence as value between 0 an 1.

Wersuhn, G., Hübner, G.: Über den Einfluß von schwerem Wasser auf die Karyokinese in der Wurzelspitze von Vicia faba. Flora (Jena) 154, 393–399 (1964).

—, Beevers, H.: Development and decline of the glyoxylate cycle enzymes in watermelon seedlings. (Citrullus vulgaris Schrad.). Effects of dactinomycin and cycloheximide. Z. Pflanzenphysiol. 55, 405–414 (1966).

Henderson, R. F., Henderson, T. R.: Effects of D2O on the allosteric properties of bovine liver glutamic dehydrogenase. Arch. Biochem. 129, 86–93 (1969).