Lightsheet microscopes - MPI for Brain Research - lightsheet microscope

 2024-10-12 01:41:25

A handheld high resolution shot with the OM-D EM1 mk3 Processed with OM Workspace. This results in a 50mp file. Zoom in beneath the clouds, then down to the grass.

Not as sharp and clear as most of my images - the egret was 300m away; the image is a crop of a 50MP handheld highres capture.

Saying goodbye to the sun and sliding into the night. Thanks for all the faves and comments, even for just taking the time to view my photo's, much appreciated.

Here's a new one for me. He was so far away I couldn't even see color till I looked through my viewfinder at 840mm. Then I cropped a bunch - nice to have 50mp to start with. My birdie book describes him as "common in any brushy or weedy habitat, especially along streams in arid regions." That pretty well describes it, except for the "common" part. A first for me at my local regional park, or anywhere for that matter.

What is confocal microscopyused for

Confocal microscopes, sometimes referred to as laser scanning confocal microscopes, take advantage of the properties of light to produce images at a single optical plane through thick specimens. To accomplish this, confocal microscopes rely on the property of certain molecules to produce fluorescence, and on the ability to collect this fluorescence from the plane of focus while rejecting all other fluorescence.

Confocal microscopydiagram

Een schaapskudde in Heerde doet zich tegoed aan de in bloei staande heide in natuurgebied Renderklippen || A sheep herd in Heerde feasts on the in blooming heather in nature reserve Renderklippen

on a cold but even so great winter morning where the rising sun melts the ice and give some color to this little village of mine.

Disadvantages ofconfocal microscopy

The typically 12-16MP images you get are missing a lot of real details (besides the fake sharpness impression). The 50MP these sensors can also produce look good at afirst glance but a closer look shows the same problems as with their smaller siblings (lot of noise reduction and ege "enhancement" after that). But when you scale down the 200MP to half the resolution resulting in also 50MP you get a quite pleasing look with much more real details.

Laser scanningconfocal microscopy

Over the dyke under a blue sky with white clouds and a shining sunUsed an old Canon EF 35-135mm f/4-5.6 USM lens, after more then 25 years still doing a good job. Thanks for all the faveds and comments, even for just taking the time to view my photo's, much appreciated.

on a cloudy summer evening. Does anyone knows why a 50 mp picture is coming out of lightroom at only 16 mb, please give me some insight, thanks.

My brother's camera gave exquisite photos of 50Mp. Wish I had the original. His photo files for one picture are huge. On his very large television screen, you could see every fibre of the feathers even when you zoomed in and made the bird as big as the screen, the detail was amazing. My photo shows more of a blur because the televison screen had shimmering lines all the time.

In addition to scanning the specimen in the X and Y dimensions, confocal microscopes can control the focal plane by raising and lowering the microscope stage. Using a stepper motor, the stage can be stepped in tiny increments (0.1 microns) through a sample. The software controlling the microscope can store the image information as it steps through a sample, allowing a true three-dimensional analysis of specimens.

Confocal microscopyppt

Founded in 1246 by the Earl of Cornwall, Hailes Abbey is set amid delightful Cotswold countryside. Once the centre of monastic life, the tranquil ruins are now the perfect place to relax and enjoy a picnic in a unique historic setting. Visit the new museum to discover the treasures of Hailes, uncovering stories of the monks who lived and worshipped at the abbey for nearly three centuries.

on a warm winter day. Thanks for all the faves and comments, even for just taking the time to view my photo's, much appreciated.

My brother was showing his photos on the television. I took a photo of his photo. I can't remember the name of this finch. My brother's camera gave exquisite photos of 50Mp. Wish I had the original. His photo files for one picture are huge. On his very large television screen, you could see every fibre of the feathers even when you zoomed in and made the bird as big as the screen, the detail was amazing. My photo shows more of a blur because the televison screen had shimmering lines all the time. If I get the original I will post it to show the image he actually captured.

Not as sharp and clear as most of my images - the egret was 300m away; the image is a crop of a 50MP handheld highres capture.

Samrtphone cameras can produce already 200MP images. Typically this output is way too high to directly post it. So why even shoot images in this resolution? The typically 12-16MP images you get are missing a lot of real details (besides the fake sharpness impression). The 50MP these sensors can also produce look good at afirst glance but a closer look shows the same problems as with their smaller siblings (lot of noise reduction and ege "enhancement" after that). But when you scale down the 200MP to half the resolution resulting in also 50MP you get a quite pleasing look with much more real details.

on a cold but even so great winter morning where the rising sun melts the ice and give some color to this little village of mine. Thanks for all the faves and comments, even for just taking the time to view my photo's, much appreciated.

Samrtphone cameras can produce already 200MP images. Typically this output is way too high to directly post it. So why even shoot images in this resolution?

This property of certain molecules to produce fluorescence at a very particular wavelength (called “emission”) after absorbing light of a specific wavelength (called “excitation”) has been exploited for decades by traditional fluorescence microscopy. There is a mind-boggling assortment of dyes that localize to specific cellular structures or organelles, can respond to changes in ion concentrations, or can be attached to almost any antibody. Since the mid-1990’s, it has become possible to cause a cell to manufacture its own fluorescent molecule. By introducing a jellyfish gene coding for a molecule called Green Fluorescent Protein (GFP), researchers produced a tool capable of “reporting” on the location and timing of expression of any gene of interest.

Een schaapskudde in Heerde doet zich tegoed aan de in bloei staande heide in natuurgebied Renderklippen || A sheep herd in Heerde feasts on the in blooming heather in nature reserve Renderklippen

Photo taken in Cornwall UK at the Tamar river by Cotehele Quay, a very quiet area with attractive photo spots and incredibly beautiful light. Just in the tree line is Lime Klins.

Confocal microscopyprinciple

Proof that I have a softer side, regretfully the proof doesn’t extend to me actually knowing the species of flower but I do know that I shot it handheld using the Olympus’ 50mp high res mode

Many molecules have the ability to absorb certain wavelengths of light. For example, plant leaves appear green because the chlorophyll molecules in the leaf absorb wavelengths of light in the blue and red region of the spectrum. After subtracting the blue and red light from sunlight, much of the visible light that remains is green. Once absorbed, the light energy can be converted into chemical energy (photosynthesis) or into heat. Some molecules have the ability to re-emit light energy, and this process is called fluorescence. The light that is emitted always contains less energy than the light that was absorbed (remember thermodynamics?), and is thus always at a longer wavelength.

Confocal microscopyPDF

Is confocal microscopylightmicroscopy

This was taken at my local country park the dog leapt off a fishing staging after a ball that had been thrown in by its owner. Its a rescue dog by the way :)

Photo taken in Cornwall UK at the Tamar river by Cotehele Quay, a very quiet area with attractive photo spots and incredibly beautiful light. Just in the tree line is Lime Klins.

Sited to the right of Derby cathedral entrance. This is a 50mp handheld hi-res from the Olympus EM1.3 and the Zuiko 17mm f1.2

This was taken at my local country park the dog leapt off a fishing staging after a ball that had been thrown in by its owner. Its a rescue dog by the way :)

Proof that I have a softer side, regretfully the proof doesn’t extend to me actually knowing the species of flower but I do know that I shot it handheld using the Olympus’ 50mp high res mode

While traditional fluorescence microscopy provides useful information about the localization of fluorescent compounds, the images produced can look blurry or out of focus. This is because the emitted light that is collected to form the image originate from any optical plane within the specimen. To overcome this limitation, confocal microscopy was developed. Confocal microscopy uses light from a laser through the objective of a standard light microscope to excite a specimen within a narrow plane of focus. Any emission of light from out-of-focus planes is rejected by the pinhole, or confocal aperture. A simplified lightpath for a confocal microscope is illustrated below. Only light that passes through the aperture contributes to the image formed by the photomultiplier tube. A photomultiplier tube is an extremely sensitive device for converting photons into an electrical signal.