Types ofobjectivelenses

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The objective lenses on most compound microscopes typically come in 4x, 10x, 40x, and 100x magnifications. Some microscopes may also have a 60x or 150x objective.

These lower magnification objectives provide a wide field of view which is useful for scanning slides and locating specimens. Resolution and fine detail are low, but the overview of the sample is clear.

Lower magnification gives a wide field of view to find and identify organisms. Medium magnifications like 400x are ideal for studying cell structure. 1000x is used for finer details like organelles.

High powerobjective lens

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Objective lensmicroscope function

You will notice that the lower power lenses (4X and 10X) are shorter than the high power lenses (40X and 100X), meaning that the clearance between the objective ...

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10xobjective lens

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4x objective lensname

The 4x scanning objective has a numerical aperture of 0.10 and a focal length of 16mm. It has a field of view of 5mm across.

It has a very high 1.25 numerical aperture and a short focal length of 0.2mm. At 100x the field of view is only 0.2mm wide.

Start with the lowest power 4x or 10x objective to locate the specimen. Then increase magnification to observe specific structures. Use the 100x oil immersion lens for the finest details.

Objective lensmicroscope

Some microscopes may also have specialized oil immersion objectives like 60x or 150x. Like the 100x lens, these objectives require immersion oil to reach their maximum NA and magnifying power. Immersion oils are specially formulated to have specific refractive indexes matched to the lens.

To achieve the full NA of 1.25, immersion oil is placed between the slide and the 100x objective. This prevents refraction and loss of resolution from the air-glass interface.

Objective lens 4xused

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The 10x objective lens typically has a numerical aperture of 0.25 and a focal length of 4mm. The field of view at 10x is around 2mm across.

The high NA paired with oil immersion allows the 100x objective to see fine subcellular details like organelles and chromosomes. It is used to study cellular structure and mitosis.

Prism Based on Shape of Bases · Triangular prism (has triangular bases). · Square prism (has square bases). · Rectangular prism (has rectangular bases).

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