11. 5 min scarlet-fuchsin Scarlet-fuchsin: 90 ml of 1% of Biebrich scarlet (C.I. 26905), diluted in distilled H2O. 9 ml of 1 % of acid fuchsin (C.I. 42685), diluted distilled in H2O 1 ml of glacial acetic acid

Lenticular lens

Georges-Louis Leclerc de Buffon (1748) originated the idea of dividing a lens surface into concentric rings in order to reduce the weight significantly. In 1821 this idea was adopted by Augustin-Jean Fresnel in the construction of lighthouse lenses.

20181220 — This is the range of what a user can see. For example, your FOV in a video game is how much of a given level you can see on your screen at once.

Fresnelscreen

17. Quick dehydration. Several seconds in 80º, 96º y 100º ethanol The 96 º ethanol step has to be no longer than a few seconds because the light green staining progressively disappears. At the same time, scarlet-fuchsin staining changes a bit to a more light and pinky color. A very good staining depends a lot on properly adjusting the time in this step. If the staining is not good enough, the slides can go to previous steps of the protocol by re-hydrating the tissues.

A wide variety of thin Fresnel lenses are molded in plastic, the width of the rings being only a few thousandths of an inch, for use as field lenses with ground-glass screens in cameras and small projectors to increase the brightness of the outer parts of the screen.

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FresnelEffect

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Cylindrical lens

15. several seconds in distilled H2O The staining quality can be checked under the microscope. If it is not good enough, sections can go back and be stained for a longer time in the light green dye, but always with a previous step in phosphomolibdic acid. The dehydration steps with acidic water and 96 º ethanol can be extended if the staining is too strong.

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Samples are fixed in Bouin and paraffin embedded. 8 µm thick sections are obtained and attached to gelatin coated slides.

13. 15 min 5 % of phosphomolibdic acid 5% diluted in distilled H2O This step is necessary for a proper light green dye staining. The phosphomolibdic solution has to be fresh, and it should not be used many times. Fuchsin and scarlet is also removed in this step, and therefore it must be considered during previous staining processes checks. The sections can go back to previous steps in the color is lost.

9. 5 min tap water for differentition The staining process can be checked at the microscope. If the color is not strong enough, sections can go back to Weigert hematoxylin.

Fresnellens

Sections can go back to previous steps to improve the quality of the staining, but no more than 3 o 4 times because the tissue progressively loses the capacity to retain the color.

12. 2 min distilled H2O In this step, the process of staining can be checked. However, keep in mind that differentiation is done is a forward step. If the color is weak, sections can go back to scarlet-fuchsin solution. If the color is too strong, sections can stay longed in distilled H2O.

Fresnellight

The focal length of a microscope objective is typically between 2 mm and 40 mm. However, that parameter is often considered as less important, since ...

A one-piece molded-glass Fresnel lens is convenient for spotlights, floodlights, railroad and traffic signals, and decorative lights in buildings. Cylindrical Fresnel lenses are used in shipboard lanterns to increase visibility.

Fresnelzone plate

Mar 30, 2021 — In this case, the microscope resolution is said to be pixel-limited, and is given by, at least, twice the pixel size scaled by the magnification ...

Fresnel lens, succession of concentric rings, each consisting of an element of a simple lens, assembled in proper relationship on a flat surface to provide a short focal length. The Fresnel lens is used particularly in lighthouses and searchlights to concentrate the light into a relatively narrow beam. It would be almost impossible to make a large lighthouse lens of the usual solid glass-disk type because the thickness and weight would be prohibitive; the lighter Fresnel lens is constructed of elements that are separately ground and polished from suitable glass blanks and assembled to make up the complete lens.

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16. 3 min 1% glacial acetic acid in distilled H2O. Differentiation. 1 min is enough if the sections are not strongly stained. More time may fade out the color from the tissues. demasiado.

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Trichrome means the three dyes used in this protocol: hematoxylin, fuchsin and light green. Masson trichrome is a very useful staining for distinguish collagen fibers, and connective tissue in general, from muscle fibers and epithelium. It is used for tumoral processes diagnosis. There are many variations of this staining protocol, which have been adapted to particular needs.

6. 5 min distilled H2O If tissues are not fixed in Bouin, it is a good practice to incubate sections in Bouin fixative for 24 h at room temperature, or 1 h at 56 - 60 ºC. Bouin works as mordant.te, durante 24 h a temperatura ambiente o 1 h a 56 - 60 ºC.

14. 10 min 2 % light green (CI 42095) (it can be replaced by anline blue) 2 g Light green (CI 42095), 2 ml glacial acetic acid and 98 ml distilled H2O

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