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Fluorophoresin fluorescence microscopy

Correction 12 June 2024: This Technology feature misstated the nature of the protein tag used in SNAP-tag and HaloTag. It also misnamed the process used to differentiate fluorophores.

Fluorescence microscopy is one of the most powerful tools in the life-sciences toolkit. The unique spectral properties of fluorescent dyes and proteins, collectively called fluorophores, have allowed scientists to capture snapshots — and even movies — of the microscopic universe that makes biology tick. Yet scientists are still perplexed by the fundamental chemistry that keeps the lights on.

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Fluorophoreslist

Fluorophoresexamples

An illustration of green fluorescent protein with its characteristic β-barrel. Credit: Sci-Comm Studios/Science Photo Library

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