A common question when viewing a microscopy image on a large screen is: What is the total magnification I am viewing on my monitor?" In order to correctly calculate the answer to this question, we must know the answer to the following four questions.

Total magnification of40x

The next number we need to determine is the diagonal measurement of the monitor in mm (3). Typically a monitor's measurement is provided in inches. We will just simply convert the inches measurement to mm. For example, if you are using a 19" monitor, multiply this number by 25.4 to convert it to mm. 19" x 25.4 = 482.6mm diagonal monitor measurement.

365nM UV generate less unwanted “Interference Light” and they may naturally appear weaker or more white compared to the visible purple 395nM version. This can be misleading and should not be misinterpreted. General rule of thumb is that the more visible UV light you see the less effective it will be for detailed, faint detection work.

How to determine the total magnification of a microscopelab

365nm wavelength LED produce a near pure UV output which is the optimum frequency for UV fluorescence. You will see far more detail and in a greater range of colours in gemstones than any other wavelength.

The microscope c-mount adapter (2) is the piece used to connect the microscope and the microscope camera. The c-mount adapter will have a number printed on the side of it. In the image at right, the c-mount adapter has a 0.5x lens built into it.

Magnification ofcompoundmicroscopeformula

If you have questions regarding calculating on-screen microscope magnification contact Microscope World and we will be happy to help.

Eye damage guaranteed from careless use. Our 365 nM UV light torch LEDs are manufactured to produce light be almost invisible to the naked eye. It is made for a specific purpose and should not be used by children or people unauthorised by you the tradesman. Invisible UV is particularly dangerous to the human eye because it cannot detect that damage is being done.

• Need a 5w 365 nM UV light torch then click here • Need a 3w 395 nM UV light torch then click here • Need a 15w 365 nM UV light torch then click here

Total magnification of microscope

The final number we will need in order to determine the on-screen magnification is the size of the camera sensor in mm (4). The size of the sensor differs slightly from the size of the chip, so the best way to find this number is to use the chart located below. The microscope camera sensor sizes are listed at the top of the chart, and the corresponding diagonal sensor size in mm is shown below.

This 3w 365 nM UV light torch (Blacklight) is powered by 4x AAA batteries. (not included  – but we recommend Alkaline for the slightly higher consistent voltage)

Total magnification of4x

Total magnificationformula

Total magnification of10x

If we are using the 19" monitor we mentioned earlier, to convert this to mm we multiple 19 x 25.4 = 482.6mm screen size.  For this example, let's say we are using a microscope camera with a 1/2" camera sensor in it. Based on the chart above we would calculate digital magnification by using 482.6mm / 8.00 = 60.325.

Now we can find the total on-screen magnification by multiplying optical magnification x digital magnification. In our example 2 x 60.325 = 120.65x on-screen magnification.

If you want maximum effect from “black light” reactive items, 365nm is the proper wavelength for this. 365nm UV light torch will activate faint traces in fluorescent objects (without the visible purple seen in a 395 nM UV light torch – click here for that version). Therefore our 365 nM UV light is more suited to close-up work and faint detection.

Dog and Cat pee/urine detection at this 365nm wavelength. Dry urine will show easily on dark surfaces, fresh urine has a bluish look.

The microscope objective lens magnification is printed on the side of the lens as shown in the image at left (1). If you are using a stereo microscope the objective lens value would be printed on the zoom knob or on the objective turret that is turned to change the microscope magnification.

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